Refine
Year of publication
Document Type
- Article (479)
- Conference Proceeding (45)
- Part of a Book (9)
- Book (2)
- Other (2)
Language
- English (537) (remove)
Keywords
- Biosensor (7)
- LAPS (4)
- hydrogen peroxide (4)
- Field-effect sensor (3)
- Label-free detection (3)
- Light-addressable potentiometric sensor (3)
- biosensors (3)
- Bacillus atrophaeus (2)
- Capacitive field-effect sensor (2)
- Raman spectroscopy (2)
- Tobacco mosaic virus (TMV) (2)
- acetoin (2)
- capacitive field-effect sensors (2)
- field-effect sensor (2)
- sterilisation (2)
- tobacco mosaic virus (TMV) (2)
- (Bio)degradation (1)
- Bacillus atrophaeus spores (1)
- Bio-Sensors (1)
- Bioabsorbable (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (537) (remove)
The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.
Penicillin detection by means of field-effect based sensors: EnFET, capacitive EIS sensor or LAPS?
(2001)