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Meitner-Auger-electron emitters have a promising potential for targeted radionuclide therapy of cancer because of their short range and the high linear energy transfer of Meitner-Auger-electrons (MAE). One promising MAE candidate is 197m/gHg with its half-life of 23.8 h and 64.1 h, respectively, and high MAE yield. Gold nanoparticles (AuNPs) that are labelled with 197m/gHg could be a helpful tool for radiation treatment of glioblastoma multiforme when infused into the surgical cavity after resection to prevent recurrence. To produce such AuNPs, 197m/gHg was embedded into pristine AuNPs. Two different syntheses were tested starting from irradiated gold containing trace amounts of 197m/gHg. When sodium citrate was used as reducing agent, no 197m/gHg labelled AuNPs were formed, but with tannic acid, 197m/gHg labeled AuNPs were produced. The method was optimized by neutralizing the pH (pH = 7) of the Au/197m/gHg solution, which led to labelled AuNPs with a size of 12.3 ± 2.0 nm as measured by transmission electron microscopy. The labelled AuNPs had a concentration of 50 μg (gold)/mL with an activity of 151 ± 93 kBq/mL (197gHg, time corrected to the end of bombardment).
In dem vorliegenden Beitrag setzt sich der Verfasser mit dem Urteil des EuGH vom 4.5.2023 (Az.: C-60/22, DSB 2023, 178) zu den Auswirkungen eines formellen Verstoßes des Verantwortlichen gegen die Pflichten aus Artt. 26, 30 DSGVO (juris: EUV 2016/679) auf die Rechtmäßigkeit der Datenverarbeitung auseinander. Nachdem zunächst der zugrunde liegende Sachverhalt und der Hintergrund des Vorlageverfahrens skizziert wurden, gibt der Verfasser einen Überblick über die wesentlichen Entscheidungsgründe des EuGH. Insbesondere stelle der EuGH hier fest, dass die Rechtmäßigkeit der Verarbeitung in Art. 6 DSGVO geregelt sei und sich eine rechtswidrige Verarbeitung daher nur aus einem Verstoß gegen die Artt. 6 ff. DSGVO ergeben könne; die Pflichten aus Art. 26 und Art. 30 DSGVO würden nicht zu den Gründen für die Rechtmäßigkeit der Verarbeitung zählen. Mit Blick auf die Praxis lasse sich, so der Verfasser abschließend, festhalten, dass die Entscheidung insofern nicht überraschend sei; jedoch sei die Feststellung, dass sich aus Verstößen gegen Art. 26 und Art. 30 DSGVO kein Verstoß gegen das Grundrecht auf den Schutz personenbezogener Daten nachweisen lasse überraschend und bedenklich. Auch überrasche es, dass der EuGH eher in einem Nebensatz feststelle, dass der Verantwortliche im Prozess aufgrund seiner Rechenschaftspflicht gegenüber Betroffenen beweisbelastet ist; ob sich die Kammer hier der möglichen Auswirkungen ihrer Ausführungen bewusst gewesen sei, bleibe fraglich.
In Deutschland verfügen mehr als 95 % aller Haushalte über einen Internetzugang und das Interesse an Smart Home Anwendungen steigt. Je vernetzter das Zuhause wird, desto mehr Devices müssen dort ihren Platz finden. WLAN Router wirken durch ihre technische Designsprache meist wie Fremdkörper im Raum, sodass oft versucht wird, sie zu verstecken. Das soll sich mit „Cobo“, dem smarten WLAN Router, ändern. Neben der Bereitstellung einer Internetanbindung wird er zum Hub für alle Smart Home Anwendungen. Als Herzstück des Zuhauses sorgt er für die Vernetzung aller Devices und schafft zudem eine Verbindung zwischen Technologie und Interior. Mit seinem minimalistischen Design und der gezielten Auswahl an Materialien und Farben fügt sich der intelligente Router in die eigenen vier Wände ein. „Cobo“ ist kein WLAN Router, der im Schuhregal versteckt werden muss, denn er wird zum Teil der Einrichtung.
Density reduction effects on the production of [11C]CO2 in Nb-body targets on a medical cyclotron
(2023)
Medical isotope production of 11C is commonly performed in gaseous targets. The power deposition of the proton beam during the irradiation decreases the target density due to thermodynamic mixing and can cause an increase of penetration depth and divergence of the proton beam. In order to investigate the difference how the target-body length influences the operation conditions and the production yield, a 12 cm and a 22 cm Nb-target body containing N2/O2 gas were irradiated using a 13 MeV proton cyclotron. It was found that the density reduction has a large influence on the pressure rise during irradiation and the achievable radioactive yield. The saturation activity of [11C]CO2 for the long target (0.083 Ci/μA) is about 10% higher than in the short target geometry (0.075 Ci/μA).
The present work aimed to study the mainstream feasibility of the deammonifying sludge of side stream of municipal wastewater treatment plant (MWWTP) in Kaster, Germany. For this purpose, the deammonifying sludge available at the side stream was investigated for nitrogen (N) removal with respect to the operational factors temperature (15–30°C), pH value (6.0–8.0) and chemical oxygen demand (COD)/N ratio (≤1.5–6.0). The highest and lowest N-removal rates of 0.13 and 0.045 kg/(m³ d) are achieved at 30 and 15°C, respectively. Different conditions of pH and COD/N ratios in the SBRs of Partial nitritation/anammox (PN/A) significantly influenced both the metabolic processes and associated N-removal rates. The scientific insights gained from the current work signifies the possibility of mainstream PN/A at WWTPs. The current study forms a solid basis of operational window for the upcoming semi-technical trails to be conducted prior to the full-scale mainstream PN/A at WWTP Kaster and WWTPs globally.
Companies often build their businesses based on product information and therefore try to automate the process of information extraction (IE). Since the information source is usually heterogeneous and non-standardized, classic extract, transform, load techniques reach their limits. Hence, companies must implement the newest findings from research to tackle the challenges of process automation. They require a flexible and robust system that is extendable and ensures the optimal processing of the different document types. This paper provides a distributed microservice architecture pattern that enables the automated generation of IE pipelines. Since their optimal design is individual for each input document, the system ensures the ad-hoc generation of pipelines depending on specific document characteristics at runtime. Furthermore, it introduces the automated quality determination of each available pipeline and controls the integration of new microservices based on their impact on the business value. The introduced system enables fast prototyping of the newest approaches from research and supports companies in automating their IE processes. Based on the automated quality determination, it ensures that the generated pipelines always meet defined business requirements when they come into productive use.
In comparison to single-analyte devices, multiplexed systems for a multianalyte detection offer a reduced assay time and sample volume, low cost, and high throughput. Herein, a multiplexing platform for an automated quasi-simultaneous characterization of multiple (up to 16) capacitive field-effect sensors by the capacitive–voltage (C–V) and the constant-capacitance (ConCap) mode is presented. The sensors are mounted in a newly designed multicell arrangement with one common reference electrode and are electrically connected to the impedance analyzer via the base station. A Python script for the automated characterization of the sensors executes the user-defined measurement protocol. The developed multiplexing system is tested for pH measurements and the label-free detection of ligand-stabilized, charged gold nanoparticles.
Die Kultur der Tana Toraja haben einen ganz besonderen Umgang mit dem Tod. Verstorbene werden bis zu der eigentlichen Bestattung als „krank“ angesehen und solange im Alltag mit eingebunden, ganz so als ob es sich um eine noch lebende Person handelt. Der Totenritus kann mehrere Tage andauern und Familie und Freund reisen aus dem ganzen Land herbei, um dem Fest beizuwohnen. Hier geht niemand einsam und das Sterben ist sogar ein wichtiger Bestandteil des Lebens. Studien aus Deutschland hingegen zeigen, dass zunehmend einsamer gestorben wird. Außerdem ist der Tod weiterhin ein Tabuthema. Genau dort setzt die Ausstellung und die entstandene Publikation über die Toraja-Kultur an. Einblicke in den Umgang mit dem Tod anderer Länder regen zu Reflexion und Diskurs an. Gestalterisch gibt es immer wieder Kontaktpunkte, die das Zusammenspiel der Lebenden und dem Jenseits widerspiegeln.
Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.
This work presents the Multi-Bees-Tracker (MBT3D) algorithm, a Python framework implementing a deep association tracker for Tracking-By-Detection, to address the challenging task of tracking flight paths of bumblebees in a social group. While tracking algorithms for bumblebees exist, they often come with intensive restrictions, such as the need for sufficient lighting, high contrast between the animal and background, absence of occlusion, significant user input, etc. Tracking flight paths of bumblebees in a social group is challenging. They suddenly adjust movements and change their appearance during different wing beat states while exhibiting significant similarities in their individual appearance. The MBT3D tracker, developed in this research, is an adaptation of an existing ant tracking algorithm for bumblebee tracking. It incorporates an offline trained appearance descriptor along with a Kalman Filter for appearance and motion matching. Different detector architectures for upstream detections (You Only Look Once (YOLOv5), Faster Region Proposal Convolutional Neural Network (Faster R-CNN), and RetinaNet) are investigated in a comparative study to optimize performance. The detection models were trained on a dataset containing 11359 labeled bumblebee images. YOLOv5 reaches an Average Precision of AP = 53, 8%, Faster R-CNN achieves AP = 45, 3% and RetinaNet AP = 38, 4% on the bumblebee validation dataset, which consists of 1323 labeled bumblebee images. The tracker’s appearance model is trained on 144 samples. The tracker (with Faster R-CNN detections) reaches a Multiple Object Tracking Accuracy MOTA = 93, 5% and a Multiple Object Tracking Precision MOTP = 75, 6% on a validation dataset containing 2000 images, competing with state-of-the-art computer vision methods. The framework allows reliable tracking of different bumblebees in the same video stream with rarely occurring identity switches (IDS). MBT3D has much lower IDS than other commonly used algorithms, with one of the lowest false positive rates, competing with state-of-the-art animal tracking algorithms. The developed framework reconstructs the 3-dimensional (3D) flight paths of the bumblebees by triangulation. It also handles and compares two alternative stereo camera pairs if desired.