Refine
Year of publication
- 2021 (12) (remove)
Institute
- Fachbereich Chemie und Biotechnologie (12) (remove)
Keywords
- Principal component analysis (2)
- Authenticity (1)
- Deuterated solvents (1)
- Deuterium NMR (1)
- Heparin (1)
- IR (1)
- IR spectroscopy (1)
- Linear discriminant analysis (1)
- Manufacturer (1)
- Soft independent modeling of class analogy (1)
An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.