Refine
Year of publication
- 2017 (154) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (61)
- IfB - Institut für Bioengineering (32)
- INB - Institut für Nano- und Biotechnologien (26)
- Fachbereich Elektrotechnik und Informationstechnik (25)
- Fachbereich Luft- und Raumfahrttechnik (22)
- Fachbereich Energietechnik (14)
- Fachbereich Chemie und Biotechnologie (12)
- Fachbereich Maschinenbau und Mechatronik (10)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (10)
- Fachbereich Bauingenieurwesen (7)
Language
- English (154) (remove)
Document Type
- Article (72)
- Conference Proceeding (66)
- Part of a Book (12)
- Other (2)
- Book (1)
- Patent (1)
Keywords
- MASCOT (2)
- 3D nonlinear finite element model (1)
- Acceptance tests (1)
- Automated Optimization (1)
- Autonomous mobile robots (1)
- Biomolecular logic gate (1)
- CNOT (1)
- Capacitive field-effect (1)
- Chemical images (1)
- Chemical sensor (1)
- Chimeric liver-humanized mice (1)
- Competence Developing Games (1)
- DNA (1)
- Dehydrogenase (1)
- Diaphorase (1)
- Diversity (1)
- Diversity Management (1)
- Drug distribution (1)
- Drug metabolism (1)
- Dry-low-NOx (DLN) combustion (1)
- EIS capacitive sensor (1)
- Effective modal mass (1)
- Electrolyte–insulator–semiconductor (1)
- Enterprise architecture (1)
- Enterprise transformation (1)
- Enzymatic biosensor (1)
- Enzyme biosensor (1)
- Enzyme logic gate (1)
- Field-effect sensor (1)
- GOSSAMER-1 (1)
- Gamification (1)
- Gold nanoparticle (1)
- Gossamer (1)
- High hydrogen combustion (1)
- Higher education (1)
- Hotelling’s T² test (1)
- Hydrogen combustion (1)
- Hydrogen gas turbine (1)
- Image Database (1)
- Image Forensics (1)
- Industry 4.0 (1)
- Innovation (1)
- Innovation Management (1)
- Knockout mice (1)
- LAPS (1)
- Leadership (1)
- Light-addressable potentiometric sensor (1)
- Map (eTOM) Process reference model Process design Telecommunications industry (1)
- Micromix combustion (1)
- Mixed-Methods Research (1)
- Mobile Phones (1)
- Multi-robot systems (1)
- Multiphase (1)
- Natural frequency (1)
- Negative impedance convertor (1)
- O2 plasma (1)
- Optimal Topology (1)
- PHILAE (1)
- Performance measurement (1)
- Piecewise Linearization (1)
- Plant efficiency (1)
- Process design (1)
- Process reference model (1)
- Quantitative research (1)
- Reference modelling (1)
- Resonance-mode measurement (1)
- RoboCup (1)
- Sequence-Search (1)
- Serious Games (1)
- Similarity Theory (1)
- Simultaneous determination (1)
- Small Solar System Body Lander (1)
- Small Spacecraft (1)
- Smart factory (1)
- Solar Power Sail (1)
- Students (1)
- Suspension bridge (1)
- TM Forum (1)
- Telecommunication (1)
- Thin shell finite elements (1)
- Toxicology (1)
- Trajectory Optimization (1)
- Transition (1)
- Ventilation System (1)
- XOR (1)
- annealing (1)
- asteroid sample return (1)
- change management (1)
- churches (1)
- complete block symmetry (1)
- diversity management (1)
- earthquake engineering (1)
- elastomeric bearing (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- engineering (1)
- enhanced Telecom Operations Map (eTOM) (1)
- equivalent stiffness (1)
- friction pendulum bearing (1)
- granular silo (1)
- hydroxylation (1)
- hypoplasticity (1)
- innovation management (1)
- learning theories (1)
- likelihood ratio test (1)
- liquid storage tank (1)
- macro-element (1)
- motivation theories (1)
- multiple NEA rendezvous (1)
- nonlinear transient analyses (1)
- research association (1)
- seismic isolation (1)
- simplified approach (1)
- solar sail (1)
- surface functionalization (1)
- truss (1)
- uniformly most powerful invariant test (1)
- vault (1)
Is part of the Bibliography
- no (154)
The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided.
Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM−1 (L-malate biosensor) and 0.4 μA mM−1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0–10.0 mM with a sensitivity of 0.09 μA mM−1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.
An enzyme-based reversible Controlled NOT (CNOT) logic gate operating on a semiconductor transducer
(2017)
An enzyme-based biocatalytic system mimicking operation of a logically reversible Controlled NOT (CNOT) gate has been interfaced with semiconductor electronic transducers. Electrolyte–insulator–semiconductor (EIS) structures have been used to transduce chemical changes produced by the enzyme system to an electronically readable capacitive output signal using field-effect features of the EIS device. Two enzymes, urease and esterase, were immobilized on the insulating interface of EIS structure producing local pH changes performing XOR logic operation controlled by various combinations of the input signals represented by urea and ethyl butyrate. Another EIS transducer was functionalized with esterase only, thus performing Identity (ID) logic operation for the ethyl butyrate input. Both semiconductor devices assembled in parallel operated as a logically reversible CNOT gate. The present system, despite its simplicity, demonstrated for the first time logically reversible function of the enzyme system transduced electronically with the semiconductor devices. The biomolecular realization of a CNOT gate interfaced with semiconductors is promising for integration into complex biomolecular networks and future biosensor/biomedical applications.
In this paper we propose a stochastic programming method to analyse limit and shakedown of structures under uncertainty condition of strength. Based on the duality theory, the shakedown load multiplier formulated by the kinematic theorem is proved actually to be the dual form of the shakedown load multiplier formulated by static theorem. In this investigation a dual chance constrained programming algorithm is developed to calculate simultaneously both the upper and lower bounds of the plastic collapse limit and the shakedown limit. The edge-based smoothed finite element method (ES-FEM) with three-node linear triangular elements is used for structural analysis.