Refine
Year of publication
Institute
- Fachbereich Medizintechnik und Technomathematik (1689)
- Fachbereich Elektrotechnik und Informationstechnik (718)
- IfB - Institut für Bioengineering (624)
- Fachbereich Energietechnik (588)
- INB - Institut für Nano- und Biotechnologien (557)
- Fachbereich Chemie und Biotechnologie (551)
- Fachbereich Luft- und Raumfahrttechnik (497)
- Fachbereich Maschinenbau und Mechatronik (279)
- Fachbereich Wirtschaftswissenschaften (217)
- Solar-Institut Jülich (165)
Language
- English (4909) (remove)
Document Type
- Article (3277)
- Conference Proceeding (1162)
- Part of a Book (191)
- Book (144)
- Doctoral Thesis (30)
- Conference: Meeting Abstract (28)
- Patent (25)
- Other (10)
- Report (9)
- Conference Poster (6)
Keywords
- Biosensor (25)
- Finite-Elemente-Methode (12)
- Einspielen <Werkstoff> (10)
- CAD (8)
- civil engineering (8)
- Bauingenieurwesen (7)
- Blitzschutz (6)
- FEM (6)
- Gamification (6)
- Limit analysis (6)
ε-Fe3N has been investigated by time-of-flight neutron diffraction (temperature range 4.2–618 K) and SQUID magnetometry (2–700 K, B≤5 T). A ferromagnetic spin structure is observed with magnetic moments oriented perpendicular to the c-axis of the hexagonal nuclear structure. The magnetic saturation moment of iron is 2.2 μB at 4.2 K from neutron diffraction and 2.0 μB from magnetic measurements and decreases in a Brillouin-like manner on heating to TC=575 K. Above 450 K an increasing but reversible disorder of the nitrogen partial structure is observed.
Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas®), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security.
All cells generate contractile tension. This strain is crucial for mechanically controlling the cell shape, function and survival. In this study, the CellDrum technology quantifying cell's (the cellular) mechanical tension on a pico-scale was used to investigate the effect of lipopolysaccharide (LPS) on human aortic endothelial cell (HAoEC) tension. The LPS effect during gram-negative sepsis on endothelial cells is cell contraction causing endothelium permeability increase. The aim was to finding out whether recombinant activated protein C (rhAPC) would reverse the endothelial cell response in an in-vitro sepsis model. In this study, the established in-vitro sepsis model was confirmed by interleukin 6 (IL-6) levels at the proteomic and genomic levels by ELISA, real time-PCR and reactive oxygen species (ROS) activation by florescence staining. The thrombin cellular contraction effect on endothelial cells was used as a positive control when the CellDrum technology was applied. Additionally, the Ras homolog gene family, member A (RhoA) mRNA expression level was checked by real time-PCR to support contractile tension results. According to contractile tension results, the mechanical predominance of actin stress fibers was a reason of the increased endothelial contractile tension leading to enhanced endothelium contractility and thus permeability enhancement. The originality of this data supports firstly the basic measurement principles of the CellDrum technology and secondly that rhAPC has a beneficial effect on sepsis influenced cellular tension. The technology presented here is promising for future high-throughput cellular tension analysis that will help identify pathological contractile tension responses of cells and prove further cell in-vitro models.