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Year of publication
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Keywords
- LAPS (4)
- Natural language processing (4)
- CellDrum (3)
- Field-effect sensor (3)
- Light-addressable potentiometric sensor (3)
- Paired sample (3)
- hydrogen peroxide (3)
- impedance spectroscopy (3)
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Λ, Image production in e+e- annihilation at 33 GeV centre of mass energy. TASSO Collaboration
(1981)
π0 production by e+e− annihilation at 14 and 34 GeV c.m. energy . TASSO Collaboration
(1982)
Buniatyan, V. V. ; Matirosyan, N. W. ; Abouzar, Maryam H. ; Schöning, Michael Josef ; Poghossian, Arshak ; Schubert, J. ; Khachatryan, V. R. ; Soukiassyan, G. R.
Yoshinobu, Tatsuo ; Krause, Steffi ; Miyamoto, Ko-ichiro ; Werner, Frederik ; Poghossian, Arshak ; Wagner, Torsten ; Schöning, Michael Josef
The light-addressable potentiometric sensor (LAPS) and scanning photo-induced impedance microscopy (SPIM) are two closely related methods to visualise the distributions of chemical species and impedance, respectively, at the interface between the sensing surface and the sample solution. They both have the same field-effect structure based on a semiconductor, which allows spatially resolved and label-free measurement of chemical species and impedance in the form of a photocurrent signal generated by a scanning light beam. In this article, the principles and various operation modes of LAPS and SPIM, functionalisation of the sensing surface for measuring various species, LAPS-based chemical imaging and high-resolution sensors based on silicon-on-sapphire substrates are described and discussed, focusing on their technical details and prospective applications.
Muschallik, Lukas ; Molinnus, Denise ; Bongaerts, Johannes ; Pohl, Martina ; Wagner, Torsten ; Schöning, Michael Josef ; Siegert, Petra ; Selmer, Thorsten
The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development.
Langen, Karl J. ; Ziemons, Karl ; Kiwit, Jürgen C. W. ; Herzog, Hans ; Kuwert, Torsten ; Bock, Wolfgang ; Stöcklin, Gerhard ; Feinendegen, Ludwig E. ; Müller-Gärtner, Hans-W.
Kaminsky, Radoslav ; Kallweit, Stephan ; Weber, Hans-Joachim ; Simons, Antoine ; Kramm, K. ; Jazwik, K. ; Moll, J. ; Verdonck, P.
Achtsnicht, Stefan ; Tödter, Julia ; Niehues, Julia ; Telöken, Matthias ; Offenhäusser, Andreas ; Krause, Hans-Joachim ; Schröper, Florian
For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.
Jansen, Sebastian ; Behbahani, Mehdi ; Laumen, Marco ; Kaufmann, Tim ; Hormes, Marcus ; Schmitz-Rode, Thomas ; Behr, Marek ; Steinseifer, Ulrich
468 Scatter dose determination at the eye lens during a mask based whole brain radiotherapy (WBRT)
(2005)
Schöning, Michael Josef ; Simonis, Anette ; Ruge, Christian ; Ecken, Holger ; Müller-Veggian, Mattea ; Lüth, Hans
Macroporous silicon has been etched from n-type Si, using a vertical etching cell where no rear side contact on the silicon wafer is necessary. The resulting macropores have been characterised by means of Scanning Electron Microscopy (SEM). After etching, SiO₂ was thermally grown on the top of the porous silicon as an insulating layer and Si₃N₄ was deposited by means of Low Pressure Chemical Vapour Deposition (LPCVD) as transducer material to fabricate a capacitive pH sensor. In order to prepare porous biosensors, the enzyme penicillinase has been additionally immobilised inside the porous structure. Electrochemical measurements of the pH sensor and the biosensor with an Electrolyte/Insulator/Semiconductor (EIS) structure have been performed in the Capacitance/Voltage (C/V) and Constant capacitance (ConCap) mode.
Schöning, Michael Josef ; Schütz, S. ; Schroth, P. ; Weißbecker, B. ; Steffen, A. ; Kordos, P. ; Hummel, Hans E.
Schöning, Michael Josef ; Schütz, S. ; Riemer, A. ; Weißbecker, B. ; Steffen, A. ; Kordos, P. ; Lüth, H. ; Hummel, Hans E.
Schöning, Michael Josef ; Crott, M. ; Ronkel, F. ; Thust, M. ; Schultze, J. W. ; Kordos, P. ; Lüth, H.
A Classical Reformulation of Finite-Dimensional Quantum Mechanics. Hellwig, K.-E.; Stulpe, W.
(1993)
The readout of gamma detectors is considerably simplified when the event intensity is encoded as a pulse width (Pulse Width Modulation, PWM). Time-to-Digital-Converters (TDC) replace the conventional ADCs and multiple TDCs can be realized easily in one PLD chip (Programmable Logic Device). The output of a PWM stage is only one digital signal per channel which is well suited for transport so that further processing can be performed apart from the detector. This is particularly interesting for large systems with high channel density (e.g. high resolution scanners). In this work we present a circuit with a linear transfer function that requires a minimum of components by performing the PWM already in the preamp stage. This allows a very compact and also cost-efficient implementation of the front-end electronics.
A nonparametric goodness-of-fit test for random variables with values in a separable Hilbert space is investigated. To verify the null hypothesis that the data come from a specific distribution, an integral type test based on a Cramér-von-Mises statistic is suggested. The convergence in distribution of the test statistic under the null hypothesis is proved and the test's consistency is concluded. Moreover, properties under local alternatives are discussed. Applications are given for data of huge but finite dimension and for functional data in infinite dimensional spaces. A general approach enables the treatment of incomplete data. In simulation studies the test competes with alternative proposals.
Bohrn, Ulrich ; Mucha, Andreas ; Werner, Frederik ; Trattner, Barbara ; Bäcker, Matthias ; Krumbe, Christoph ; Schienle, Meinrad ; Stütz, Evamaria ; Schmitt-Landsiedel, Doris ; Fleischer, Maximilian ; Wagner, Patrick ; Schöning, Michael Josef
A Formulation of Quantum Stochastic Processes and Some of its Properties. Hellwig, K.-E.; Stulpe, W.
(1983)
A generalized shear-lag theory for fibres with variable radius is developed to analyse elastic fibre/matrix stress transfer. The theory accounts for the reinforcement of biological composites, such as soft tissue and bone tissue, as well as for the reinforcement of technical composite materials, such as fibre-reinforced polymers (FRP). The original shear-lag theory proposed by Cox in 1952 is generalized for fibres with variable radius and with symmetric and asymmetric ends. Analytical solutions are derived for the distribution of axial and interfacial shear stress in cylindrical and elliptical fibres, as well as conical and paraboloidal fibres with asymmetric ends. Additionally, the distribution of axial and interfacial shear stress for conical and paraboloidal fibres with symmetric ends are numerically predicted. The results are compared with solutions from axisymmetric finite element models. A parameter study is performed, to investigate the suitability of alternative fibre geometries for use in FRP.
On the basis of bivariate data, assumed to be observations of independent copies of a random vector (S,N), we consider testing the hypothesis that the distribution of (S,N) belongs to the parametric class of distributions that arise with the compound Poisson exponential model. Typically, this model is used in stochastic hydrology, with N as the number of raindays, and S as total rainfall amount during a certain time period, or in actuarial science, with N as the number of losses, and S as total loss expenditure during a certain time period. The compound Poisson exponential model is characterized in the way that a specific transform associated with the distribution of (S,N) satisfies a certain differential equation. Mimicking the function part of this equation by substituting the empirical counterparts of the transform we obtain an expression the weighted integral of the square of which is used as test statistic. We deal with two variants of the latter, one of which being invariant under scale transformations of the S-part by fixed positive constants. Critical values are obtained by using a parametric bootstrap procedure. The asymptotic behavior of the tests is discussed. A simulation study demonstrates the performance of the tests in the finite sample case. The procedure is applied to rainfall data and to an actuarial dataset. A multivariate extension is also discussed.
Wagner, Torsten ; Werner, Frederik ; Miyamoto, Ko-Ichiro ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
A high-Q resonance-mode measurement of EIS capacitive sensor by elimination of series resistance
(2017)
Miyamoto, Ko-ichiro ; Hayashi, Kosuke ; Sakamoto, Azuma ; Werner, Frederik ; Wagner, Torsten ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
An EIS capacitive sensor is a semiconductor-based potentiometric sensor, which is sensitive to the ion concentration or pH value of the solution in contact with the sensing surface. To detect a small change in the ion concentration or pH, a small capacitance change must be detected. Recently, a resonance-mode measurement was proposed, in which an inductor was connected to the EIS capacitive sensor and the resonant frequency was correlated with the pH value. In this study, the Q factor of the resonant circuit was enhanced by canceling the internal resistance of the reference electrode and the internal resistance of the inductor coil with the help of a bypass capacitor and a negative impedance converter, respectively. 1% variation of the signal in the developed system corresponded to a pH change of 3.93 mpH, which was about 1/12 of the conventional method, suggesting a better performance in detection of a small pH change.
Gossmann, Matthias ; Thomas, Ulrich ; Horváth, András ; Dragicevic, Elena ; Stoelzle-Feix, Sonja ; Jung, Alexander ; Raman, Aravind Hariharan ; Staat, Manfred ; Linder, Peter
Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.
Wagner, Torsten ; Molina, R. ; Biselli, Manfred ; Canzoneri, Michele ; Schnitzler, Thomas ; Yoshinobu, Tatsuo ; Schöning, Michael Josef
Results are presented on the ratios of the nucleon structure function in copper to deuterium from two separate experiments. The data confirm that the nucleon structure function,F 2, is different for bound nucleons than for the quasi-free ones in the deuteron. The redistribution in the fraction of the nucleon's momentum carried by quarks is investigated and it is found that the data are compatible with no integral loss of quark momenta due to nuclear effects.
The spin asymmetry in deep inelastic scattering of longitudinally polarised muons by longitudinally polarised protons has been measured over a large x range (0.01<x<0.7). The spin-dependent structure function g1(x) for the proton has been determined and its integral over x found to be 0.114±0.012±0.026, in disagreement with the Ellis-Jaffe sum rule. Assuming the validity of the Bjorken sum rule, this result implies a significant negative value for the integral of g1 for the neutron. These values for the integrals of g1 lead to the conclusion that the total quark spin constitutes a rather small fraction of the spin of the nucleon.
Moreno i Codinachs, L. ; Birkenstock, C. ; Garma, T. ; Zierold, R. ; Bachmann, J. ; Nielsch, K. ; Schöning, Michael Josef ; Fontcuberta i Morral, A.
A microscopic photometric method for measuring erythrocyte deformability. Artmann, Gerhard Michael
(1986)
Leschinger, Tim ; Birgel, Stefan ; Hackl, Michael ; Staat, Manfred ; Müller, Lars Peter ; Wegmann, Kilian
Purpose
Two semi-empirical models were recently published, both making use of existing literature data, but each taking into account different physical phenomena that trigger hemolysis. In the first model, hemoglobin (Hb) release is described as a permeation procedure across the membrane, assuming a shear stress-dependent process (sublethal model). The second model only accounts for hemoglobin release that is caused by cell membrane breakdown, which occurs when red blood cells (RBC) undergo mechanically induced shearing for a period longer than the threshold time (nonuniform threshold model). In this paper, we introduce a model that considers the hemolysis generated by both these possible phenomena.
Methods
Since hemolysis can possibly be caused by permeation of hemoglobin through the RBC functional membrane as well as by release of hemoglobin from RBC membrane breakdown, our proposed model combines both these models. An experimental setup consisting of a Couette device was utilized for validation of our proposed model.
Results
A comparison is presented between the damage index (DI) predicted by the proposed model vs. the sublethal model vs. the nonthreshold model and experimental datasets. This comparison covers a wide range of shear stress for both human and porcine blood. An appropriate agreement between the measured DI and the DI predicted by the present model was obtained.
Conclusions
The semiempirical hemolysis model introduced in this paper aims for significantly enhanced conformity with experimental data. Two phenomenological outcomes become possible with the proposed approach: an estimation of the average time after which cell membrane breakdown occurs under the applied conditions, and a prediction of the ratio between the phenomena involved in hemolysis.
A New Class of Biosensors Based on Tobacco Mosaic Virus and Coat Proteins as Enzyme Nanocarrier
(2016)
Bäcker, Matthias ; Koch, C. ; Geiger, F. ; Eber, F. ; Gliemann, H. ; Poghossian, Arshak ; Schöning, Michael Josef
A new in vitro tool to investigate cardiac contractility under physiological mechanical conditions
(2019)
Linder, Peter ; Beckler, Matthias ; Doerr, Leo ; Stoelzle-Feix, Sonja ; Fertig, Niels ; Jung, Alexander ; Staat, Manfred ; Gossmann, Matthias
Grottke, O. ; Braunschweig, T. ; Philippen, B. ; Gatzweiler, Karl-Heinz ; Gronloh, N. ; Staat, Manfred ; Rossaint, R. ; Tolba, R.
Mourzina, Y.G. ; Schöning, Michael Josef ; Schubert, J. ; Zander, W. ; Legin, A. V. ; Vlasov, Y. G. ; Kordos, P. ; Lüth, H.
Näther, Niko ; Emmerich, Rüdiger ; Berger, Jörg ; Friedrich, Peter ; Henkel, Hartmut ; Schneider, Andreas ; Schöning, Michael Josef
Uysal, Karya ; Firat, Ipek Serat ; Creutz, Till ; Aydin, Inci Cansu ; Artmann, Gerhard ; Teusch, Nicole ; Temiz Artmann, Aysegül
Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here.
Yang, Peng-Fei ; Kriechbaumer, Andreas ; Albracht, Kirsten ; Sanno, Maximilian ; Ganse, Bergita ; Koy, Timmo ; Shang, Peng ; brüggemann, Gert-Peter ; Müller, Lars Peter ; Rittweger, Jörn
A novel scheme for precise diagnostics and effective stabilization of currents in a fuel cell stack
(2010)
Schöning, Michael Josef ; Beckers, L. ; Schaub, A. ; Zander, W. ; Schubert, J. ; Mesters, S. ; Kordos, P. ; Lüth, H.
Garibaldi, F. ; Beging, Stefan ; Canese, R. ; Carpinelli, G. ; Clinthorne, N. ; Colilli, S. ; Cosentino, L. ; Finocchiaro, P. ; Giuliani, F. ; Gricia, M. ; Lucentini, M. ; Majewski, S. ; Monno, E. ; Musico, P. ; Santavenere, F. ; Tödter, J. ; Wegener, Hans-Peter ; Ziemons, Karl
Within the developments for the Crystal Clear small animal PET project (CLEARPET) a dual head PET system has been established. The basic principle is the early digitization of the detector pulses by free running ADCs. The determination of the γ-energy and also the coincidence detection is performed by data processing of the sampled pulses on the host computer. Therefore a time mark is attached to each pulse identifying the current cycle of the 40 MHz sampling clock. In order to refine the time resolution the pulse starting time is interpolated from the samples of the pulse rise. The detector heads consist of multichannel PMTs with a single LSO scintillator crystal coupled to each channel. For each PMT only one ADC is required. The position of an event is obtained separately from trigger signals generated for each single channel. An FPGA is utilized for pulse buffering, generation of the time mark and for the data transfer to the host via a fast I/O-interface.
A small PET system has been built up with two multichannel photomultipliers, which are attached to a matrix of 64 single LSO crystals each. The signal from each multiplier is being sampled continuously by a 12 bit ADC at a sampling frequency of 40 MHz. In case of a scintillation pulse a subsequent FPGA sends the corresponding set of samples together with the channel information and a time mark to the host computer. The data transfer is performed with a rate of 20 MB/s. On the host all necessary information is extracted from the data. The pulse energy is determined, coincident events are detected and multiple hits within one matrix can be identified. In order to achieve a narrow time window the pulse starting time is refined further than the resolution of the time mark (=25 ns) would allow. This is possible by interpolating between the pulse samples. First data obtained from this system will be presented. The system is part of developments for a much larger system and has been created to study the feasibility and performance of the technique and the hardware architecture.
Behbahani, Mehdi ; Behr, M. ; Hormes, M. ; Steinseifer, U. ; Arora, D. ; Coronado, O. ; Pasquali, M.
A second-order L-stable exponential time-differencing (ETD) method is developed by combining an ETD scheme with approximating the matrix exponentials by rational functions having real distinct poles (RDP), together with a dimensional splitting integrating factor technique. A variety of non-linear reaction-diffusion equations in two and three dimensions with either Dirichlet, Neumann, or periodic boundary conditions are solved with this scheme and shown to outperform a variety of other second-order implicit-explicit schemes. An additional performance boost is gained through further use of basic parallelization techniques.
Schöning, Michael Josef ; Abouzar, Maryam H. ; Wagner, Torsten ; Näther, Niko ; Rolka, David ; Yoshinobu, Tatsuo ; Kloock, Joachim P. ; Turek, Monika ; Ingebrandt, Sven ; Poghossian, Arshak
Schöning, Michael Josef ; Buß, G. ; Faßbender, F. ; Glück, O. ; Schmitt, G. ; Schultze, J. W. ; Lüth, H.
Schöning, Michael Josef ; Buß, G. ; Faßbender, F. ; Glück, O. ; Schmitt, G. ; Schultze, J. W. ; Lüth, H.
Bäcker, Matthias ; Pouyeshman, S. ; Schnitzler, Thomas ; Poghossian, Arshak ; Wagner, Patrick ; Biselli, Manfred ; Schöning, Michael Josef
We consider the numerical approximation of second-order semi-linear parabolic stochastic partial differential equations interpreted in the mild sense which we solve on general two-dimensional domains with a C² boundary with homogeneous Dirichlet boundary conditions. The equations are driven by Gaussian additive noise, and several Lipschitz-like conditions are imposed on the nonlinear function. We discretize in space with a spectral Galerkin method and in time using an explicit Euler-like scheme. For irregular shapes, the necessary Dirichlet eigenvalues and eigenfunctions are obtained from a boundary integral equation method. This yields a nonlinear eigenvalue problem, which is discretized using a boundary element collocation method and is solved with the Beyn contour integral algorithm. We present an error analysis as well as numerical results on an exemplary asymmetric shape, and point out limitations of the approach.