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A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants
(2013)
Bouwman, Peter ; Gulden, Hanneke van der ; Heijden, Ingrid van der ; Drost, Rinske ; Klijn, Christiaan N. ; Prasetyanti, Pramudita ; Pieterse, Mark ; Wientjens, Ellen ; Seibler, Jost ; Hogervorst, Frank B. L. ; Jonkers, Jos
Michalak, Ewa Malgorzata ; Nacerddine, Karim ; Pietersen, Alexandra ; Beuger, Vincent ; Pawlitzky, Inka ; Cornelissen-Steijger, Paulien ; Wientjens, Ellen ; Tanger, Ellen ; Seibler, Jost ; Lohuizen, Maarten van ; Jonkers, Jos
Gebeshuber, Christoph A. ; Kornauth, Christoph ; Dong, Lihua ; Sierig, Ralph ; Seibler, Jost ; Reiss, Martina ; Tauber, Stefanie ; Bilban, Martin ; Wang, Shijun ; Kain, Renate ; Böhmig, Georg A. ; Moeller, Marcus J. ; Gröne, Hermann-Josef ; Englert, Christoph ; Martinez, Javier ; Kerjaschki, Dontscho
Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b
(2013)
Kornfeld, Jan-Wilhelm ; Baitzel, Catherina ; Könner, A. Christine ; Nicholls, Hayley T. ; Vogt, Merly C. ; Herrmanns, Karolin ; Scheja, Ludger ; Haumaitre, Cécile ; Wolf, Anna M. ; Knippschild, Uwe ; Seibler, Jost ; Cereghini, Silvia ; Heeren, Joerg ; Stoffel, Markus ; Brüning, Jens C.
Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis
(2013)
Rachinger, Michael ; Bauch, Melanie ; Strittmatter, Axel ; Bongaerts, Johannes ; Evers, Stefan ; Maurer, Karl-Heinz ; Daniel, Rolf ; Liebl, Wolfgang ; Liesegang, Heiko ; Ehrenreich, Armin
The concept of an injective affine embedding of the quantum states into a set of classical states, i.e., into the set of the probability measures on some measurable space, as well as its relation to statistically complete observables is revisited, and its limitation in view of a classical reformulation of the statistical scheme of quantum mechanics is discussed. In particular, on the basis of a theorem concerning a non-denseness property of a set of coexistent effects, it is shown that an injective classical embedding of the quantum states cannot be supplemented by an at least approximate classical description of the quantum mechanical effects. As an alternative approach, the concept of quasi-probability representations of quantum mechanics is considered.
Niemüller, Tim ; Lakemeyer, Gerhard ; Ferrein, Alexander ; Reuter, S. ; Ewert, D. ; Jeschke, S. ; Pensky, D. ; Karras, Ulrich
In modernen Fahrzeugkarosserien der Großserie kommen zunehmend Materialmischbauweisen
zur Anwendung. In Zusammenarbeit der Daimler AG, der Tower Automotive Holding
GmbH, der Imperia GmbH sowie der Partnerunternehmen KSM Castings GmbH und Schaufler
Tooling GmbH & Co. KG wird das Leichtbaupotenzial von Stahlblech-AluminiumverbundgussHybriden
am Beispiel des vorderen Dachquerträgers des Mercedes-Benz Viano/Vito ausführlich
untersucht.
In modernen Fahrzeugkarosserien der Großserie kommen zunehmend Materialmischbauweisen zur Anwendung. In Zusammenarbeit der Daimler AG, der Tower Automotive Holding GmbH, der Imperia GmbH sowie der Partnerunternehmen KSM Castings GmbH und Schaufler Tooling GmbH & Co. KG wird das Leichtbaupotenzial von Aluminiumverbundguss-Stahlblech-Hybriden am Beispiel des vorderen Dachquerträgers des Mercedes-Benz Viano/Vito ausführlich untersucht.
Pellegrini, Paul A. ; Howell, Nicholas R. ; Shepherd, Rachael K. ; Lengkeek, Nigel A. ; Paulßen, Elisabeth ; Katsifis, Andrew G. ; Greguric, Ivan
Paulßen, Elisabeth ; Le, Van So ; Lengkeek, Nigel ; Pellegrini, Paul ; Jackson, Tim ; Greguric, Ivan ; Weiner, Ron
Der Telekommunikationsmarkt erfährt substanzielle Veränderungen. Neue Geschäftsmodelle, innovative Dienstleistungen und Technologien erfordern Reengineering, Transformation und Prozessstandardisierung. Mit der Enhanced Telecom Operation Map (eTOM) bietet das TM Forum ein international anerkanntes de facto Referenz-Prozess-Framework basierend auf spezifischen Anforderungen und Ausprägungen der Telekommunikationsindustrie an. Allerdings enthält dieses Referenz-Framework nur eine hierarchische Sammlung von Prozessen auf unterschiedlichen Abstraktionsebenen. Eine Kontrollsicht verstanden als sequenzielle Anordnung von Aktivitäten und daraus resultierend ein realer Prozessablauf fehlt ebenso wie eine Ende-zu-Ende-Sicht auf den Kunden. In diesem Artikel erweitern wir das eTOM-Referenzmodell durch Referenzprozessabläufe, in welchen wir das Wissen über Prozesse in Telekommunikationsunternehmen abstrahieren und generalisieren. Durch die Referenzprozessabläufe werden Unternehmen bei dem strukturierten und transparenten (Re-)Design ihrer Prozesse unterstützt. Wir demonstrieren die Anwendbarkeit und Nützlichkeit unserer Referenzprozessabläufe in zwei Fallstudien und evaluieren diese anhand von Kriterien für die Bewertung von Referenzmodellen. Die Referenzprozessabläufe wurden vom TM Forum in den Standard aufgenommen und als Teil von eTOM Version 9 veröffentlicht. Darüber hinaus diskutieren wir die Komponenten unseres Ansatzes, die auch außerhalb der Telekommunikationsindustrie angewandt werden können.
Umutlu, Lale ; Bitz, Andreas ; Maderwald, Stefan ; Orzada, Stephan ; Kinner, Sonja ; Kraff, Oliver ; Brote, Irina ; Ladd, Susanne C. ; Schroeder, Tobias ; Forsting, Michael
Umutlu, Lale ; Kraff, Oliver ; Fischer, Anja ; Kinner, Sonja ; Maderwald, Stefan ; Nassenstein, Kai ; Nensa, Felix ; Grüneisen, Johannes ; Orzada, Stephan ; Bitz, Andreas ; Forsting, Michael ; Ladd, Mark E. ; Lauenstein, Thomas C.
Kraff, Oliver ; Wrede, Karsten H. ; Schoemberg, Tobias ; Dammann, Philipp ; Noureddine, Yacine ; Orzada, Stephan ; Ladd, Mark E. ; Bitz, Andreas
Umutlu, L. ; Maderwald, S. ; Kinner, S. ; Kraff, O. ; Bitz, Andreas ; Orzada, S. ; Johst, S. ; Wrede, K. ; Forsting, M. ; Ladd, M. E. ; Lauenstein, T. C. ; Quick, H. H.
Wilming, Anja ; Begemann, Jens ; Kuhne, Stefan ; Regestein, Lars ; Bongaerts, Johannes ; Evers, Stefan ; Maurer, Karl-Heinz ; Büchs, Jochen
Digel, Ilya ; Akimbekov, Nuraly S. ; Turalieva, M. ; Mansurov, Z. ; Temiz Artmann, Aysegül ; Eshibaev, A. ; Zhubanova, A.
Scheele, Sandra ; Oertel, Dan ; Bongaerts, Johannes ; Evers, Stefan ; Hellmuth, Hendrik ; Maurer, Karl-Heinz ; Bott, Michael ; Freudl, Roland
Akimbekov, Nuraly S. ; Mansurov, Zulkhair ; Jandosov, J. ; Digel, Ilya ; Gossmann, Matthias ; Artmann, Gerhard ; Temiz Artmann, Aysegül ; Zhubanova, Azhar A.
The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls.
Edip, Kemal ; Garevski, Mihail ; Butenweg, Christoph ; Sesov, Vlatko ; Cvetanovska, Julijana ; Gjorgiev, Igor
The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon
(2013)
Voigt, Birgit ; Schroeter, Rebecca ; Jürgen, Britta ; Albrecht, Dirk ; Evers, Stefan ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Schweder, Thomas ; Hecker, Michael
Wiegand, Sandra ; Dietrich, Sascha ; Hertel, Robert ; Bongaerts, Johannes ; Evers, Stefan ; Volland, Sonja ; Daniel, Rolf ; Liesegang, Heiko
Krämer, Stefan ; Daab, Dominique Jonas ; Müller, Brigitte ; Wagner, Tobias ; Baader, Fabian ; Hessel, Joana ; Gdalewitsch, Georg ; Plescher, Engelbert ; Dachwald, Bernd ; Wahle, Michael ; Gierse, Andreas ; Vetter, Rudolf ; Pfützenreuter, Lysan
Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential.
Anding, Ralf ; Tabaza, Ruth ; Staat, Manfred ; Trenz, Eva ; Lohmann, Philipp ; Klinge, Uwe ; Kirschner-Hermanns, Ruth
Huck, Christina ; Schiffels, Johannes ; Herrera, Cony N. ; Schelden, Maximilian ; Selmer, Thorsten ; Poghossian, Arshak ; Baumann, Marcus ; Wagner, Patrick ; Schöning, Michael Josef
Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
Thiel, Alexander ; Tippkötter, Nils ; Suck, Kirstin ; Sohling, Ulrich ; Ruf, Friedrich ; Ulber, Roland
Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources.
Schusser, Sebastian ; Menzel, S. ; Bäcker, Matthias ; Leinhos, Marcel ; Poghossian, Arshak ; Wagner, P. ; Schöning, Michael Josef
Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes
(2013)
Micili, Serap C. ; Valter, Markus ; Oflaz, Hakan ; Ozogul, Candan ; Linder, Peter ; Föckler, Nicole ; Artmann, Gerhard ; Digel, Ilya ; Temiz Artmann, Aysegül
Einschränkung von Taluskippung und -vorschub durch Sprunggelenkorthesen nach fibularer Bandruptur
(2013)
Die fibulare Bandruptur zählt zu einer der am häufigsten auftretenden Verletzungen des
Bewegungsapparats. In den meisten Fällen wird heute die konservativ frühfunktionelle Therapie mit Sprunggelenkorthesen allgemein bevorzugt. Im Rahmen der vorliegenden Studie wurden 14 verschiedene Sprunggelenkorthesen im Hinblick auf ihre Einschränkung von Taluskippung und Talusvorschub
untersucht. Zur Simulation einer fibularen Bandruptur wurde ein Unterschenkelmodell aus Holz mit Fußteil, mit angelegten Orthesen in einen Scheuba-Halteapparat eingespannt und mit 150 N seitlich sowie anterior-posterior belastet. Anhand der erstellten "gehaltenen" Röntgenaufnahmen konnten Taluskippung und Talusvorschub jeder einzelnen Orthese eindeutig bestimmt werden. Die meisten Orthesen erreichten zufriedenstellende Ergebnisse. Es stellte sich heraus, dass vor allem eine eng anliegende, im Gelenkbereich anatomisch angepasste Form vorteilhaft zu sein scheint.
Buniatyan, V. ; Huck, Christina ; Poghossian, Arshak ; Aroutiounian, V. M. ; Schöning, Michael Josef
Impedance spectroscopy: A tool for real-time in situ monitoring of the degradation of biopolymers
(2013)
Schusser, Sebastian ; Leinhos, Marcel ; Bäcker, Matthias ; Poghossian, Arshak ; Wagner, Patrick ; Schöning, Michael Josef
Investigation of the degradation kinetics of biodegradable polymers is essential for the development of implantable biomedical devices with predicted biodegradability. In this work, an impedimetric sensor has been applied for real-time and in situ monitoring of degradation processes of biopolymers. The sensor consists of two platinum thin-film electrodes covered by a polymer film to be studied. The benchmark biomedical polymer poly(D,L-lactic acid) (PDLLA) was used as a model system. PDLLA films were deposited on the sensor structure from a polymer solution by using the spin-coating method. The degradation kinetics of PDLLA films have been studied in alkaline solutions of pH 9 and 12 by means of an impedance spectroscopy (IS) method. Any changes in a polymer capacitance/resistance induced by water uptake and/or polymer degradation will modulate the global impedance of the polymer-covered sensor that can be used as an indicator of the polymer degradation. The degradation rate can be evaluated from the time-dependent impedance spectra. As expected, a faster degradation has been observed for PDLLA films exposed to pH 12 solution.
Abulnaga, El-Hussiny ; Pinkenburg, Olaf ; Schiffels, Johannes ; E-Refai, Ahmed ; Buckel, Wolfgang ; Selmer, Thorsten
Our world is well ordered in measurement and number : or why natural constants are as they are
(2013)
All the important natural constants can be logically explained with and derived from the first four ordinal numbers, 1, 2, 3 and 4, its addition to ten and finally the standard values for obviously maximal feasibility Ω and the optimum in our world, the Golden Section (GS), i.e. the number sequences 273 and 618. They both are the first three numbers of irrational results by an arithmetical transformation of simple geometrical relationships by creating multiplicity out of singularity. Both of them show that the infinite is inherent in finiteness and explain in a simple way the smallest deviations and fluctuations between the physical AS-IS state and the obvious spiritual ideal behind: Wherever we look in this world, and especially in important key-positions, we regularly find these sequences. All of the above mentioned numbers so seem to be key players in our world, what can be demonstrated by the derivation of natural constants.
Lévesque, Mathieu ; Saurer, Matthias ; Siegwolf, Rolf ; Eilmann, Britta ; Brang, Peter ; Bugmann, Harald ; Rigling, Andreas
Gräßl, Andreas ; Renz, Wolfgang ; Hezel, Fabian ; Dieringer, Matthias A. ; Winter, Lukas ; Özerdem, Celal ; Rieger, Jan ; Kellmann, Peter ; Santoro, Davide ; Lindel, Tomasz D. ; Frauenrath, Tobias ; Pfeiffer, Harald ; Niendorf, Thoralf
Purpose
To design and evaluate a modular transceiver coil array with 32 independent channels for cardiac MRI at 7.0T.
Methods
The modular coil array comprises eight independent building blocks, each containing four transceiver loop elements. Numerical simulations were used for B1+ field homogenization and radiofrequency (RF) safety validation. RF characteristics were examined in a phantom study. The array's suitability for accelerated high spatial resolution two-dimensional (2D) FLASH CINE imaging of the heart was examined in a volunteer study.
Results
Transmission field adjustments and RF characteristics were found to be suitable for the volunteer study. The signal-to-noise intrinsic to 7.0T together with the coil performance afforded a spatial resolution of 1.1 × 1.1 × 2.5 mm3 for 2D CINE FLASH MRI, which is by a factor of 6 superior to standardized CINE protocols used in clinical practice at 1.5T. The 32-channel transceiver array supports one-dimensional acceleration factors of up to R = 4 without impairing image quality significantly.
Conclusion
The modular 32-channel transceiver cardiac array supports accelerated and high spatial resolution cardiac MRI. The array is compatible with multichannel transmission and provides a technological basis for future clinical assessment of parallel transmission techniques at 7.0T.
Eilmann, Britta ; de Vries, Sven M. G. ; den Ouden, Jan ; Mohren, Godefridus M. J. ; Sauren, Pascal ; Sass-Klaassen, Ute G. W.
The semiconductor field-effect platform represents a powerful tool for detecting the adsorption and binding of charged macromolecules with direct electrical readout. In this work, a capacitive electrolyte–insulator–semiconductor (EIS) field-effect sensor consisting of an Al-p-Si-SiO2 structure has been applied for real-time in situ electrical monitoring of the layer-by-layer formation of polyelectrolyte (PE) multilayers (PEM). The PEMs were deposited directly onto the SiO2 surface without any precursor layer or drying procedures. Anionic poly(sodium 4-styrene sulfonate) and cationic weak polyelectrolyte poly(allylamine hydrochloride) have been chosen as a model system. The effect of the ionic strength of the solution, polyelectrolyte concentration, number and polarity of the PE layers on the characteristics of the PEM-modified EIS sensors have been studied by means of capacitance–voltage and constant-capacitance methods. In addition, the thickness, surface morphology, roughness and wettabilityof the PE mono- and multilayers have been characterised by ellipsometry, atomic force microscopy and water contact-angle methods, respectively. To explain potential oscillations on the gate surface and signal behaviour of the capacitive field-effect EIS sensor modified with a PEM, a simplified electrostatic model that takes into account the reduced electrostatic screening of PE charges by mobile ions within the PEM has been proposed and discussed.
Kramer, Friederike ; Halamkova, Lenka ; Poghossian, Arshak ; Schöning, Michael Josef ; Katz, Evgeny ; Halamek, Jan
Bäcker, Matthias ; Rakowski, D. ; Poghossian, Arshak ; Biselli, Manfred ; Wagner, Patrick ; Schöning, Michael Josef
A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
Wiegand, Sandra ; Voigt, Birgit ; Albrecht, Dirk ; Bongaerts, Johannes ; Evers, Stefan ; Hecker, Michael ; Daniel, Rolf ; Liesegang, Heiko
Buniatyan, V. ; Huck, Christina ; Poghossian, Arshak ; Aroutiounian, V. M. ; Schöning, Michael Josef
Aboulnaga, El-Hussiny ; Pinkenburg, Olaf ; Schiffels, Johannes ; El-Refai, Ahmed ; Buckel, Wolfgang ; Selmer, Thorsten
Iken, Heiko ; Ahlborn, Kristina ; Gerlach, Frank ; Vonau, Winfried ; Zander, Wilhelm ; Schubert, Jürgen P. ; Schöning, Michael Josef
Murib, M. S. ; Grinsven, B. van ; Grieten, L. ; Janssens, S. D. ; Vermeeren, V. ; Eersels, K. ; Broeders, J. ; Ameloot, Marcel ; Michiels, L. ; Ceuninck, W. De ; Haenen, K. ; Schöning, Michael Josef ; Wagner, Patrick
Kuhnert, Marie-Therese ; Bialonski, Stephan ; Noenning, Nina ; Mai, Heinke ; Hinrichs, Hermann ; Helmstaedter, Christoph ; Lehnertz, Klaus
Learning- and memory-related processes are thought to result from dynamic interactions in large-scale brain networks that include lateral and mesial structures of the temporal lobes. We investigate the impact of incidental and intentional learning of verbal episodic material on functional brain networks that we derive from scalp-EEG recorded continuously from 33 subjects during a neuropsychological test schedule. Analyzing the networks' global statistical properties we observe that intentional but not incidental learning leads to a significantly increased clustering coefficient, and the average shortest path length remains unaffected. Moreover, network modifications correlate with subsequent recall performance: the more pronounced the modifications of the clustering coefficient, the higher the recall performance. Our findings provide novel insights into the relationship between topological aspects of functional brain networks and higher cognitive functions.
Werner, Frederik ; Wagner, Torsten ; Miyamoto, Ko-ichiro ; Yoshinobu, Tatsuo ; Schöning, Michael Josef
Light-addressable potentiometric sensors (LAPS) are field-effect-based sensors. A modulated light source is used to define the particular measurement spot to perform spatially resolved measurements of chemical species and to generate chemical images. In this work, an organic-LED (OLED) display has been chosen as a light source. This allows high measurement resolution and miniaturisation of the system. A new developed driving method for the OLED display optimised for LAPS-based measurements is demonstrated. The new method enables to define modulation frequencies between 1 kHz and 16 kHz and hence, reduces the measurement time of a chemical image by a factor of 40 compared to the traditional addressing of an OLED display.
Wagner, Torsten ; Werner, Frederik ; Miyamoto, Ko-Ichiro ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach to read out light-addressable potentiometric sensors (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device, which allows fast and flexible generation of light patterns. With the help of these light patterns, the sensor surface of the LAPS device can be addressed. The DLP approach has several advantages compared to conventional LAPS set-ups, e.g., the spot size and the shape of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of the measurement. In addition, the modulation frequency and intensity of the light beam are important parameters of the LAPS set-up. Within this work, the authors will discuss two different ways of light modulation by the DLP set-up, investigate the influence of different modulation frequencies and different light intensities as well as demonstrate the scanning capabilities of the new set-up by pH mapping on the sensor surface.
Miyamoto, Ko-ichiro ; Kaneko, Kazumi ; Matsuo, Akira ; Wagner, Torsten ; Kanoh, Shiníchiro ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the two-dimensional distribution of specific ions or molecules in the solution. In this study, we developed a miniaturized chemical imaging sensor system with an OLED display panel as a light source that scans the sensor plate. In the proposed configuration, the display panel is placed directly below the sensor plate and illuminates the back surface. The measured area defined by illumination can be arbitrarily customized to fit the size and the shape of the sample to be measured. The waveform of the generated photocurrent, the current–voltage characteristics and the pH sensitivity were investigated and pH imaging with this miniaturized system was demonstrated.
Realisation of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry
(2012)
Kirchner, Patrick ; Oberländer, Jan ; Friedrich, Peter ; Berger, Jörg ; Rysstad, Gunnar ; Schöning, Michael Josef ; Keusgen, Michael
A calorimetric gas sensor is presented for the monitoring of vapour-phase H2O2 at elevated temperature during sterilisation processes in aseptic food industry. The sensor was built up on a flexible polyimide foil (thickness: 25 μm) that has been chosen due to its thermal stability and low thermal conductivity. The sensor set-up consists of two temperature-sensitive platinum thin-film resistances passivated by a layer of SU-8 photo resist and catalytically activated by manganese(IV) oxide. Instead of an active heating structure, the calorimetric sensor utilises the elevated temperature of the evaporated H2O2 aerosol. In an experimental test rig, the sensor has shown a sensitivity of 4.78 °C/(%, v/v) in a H2O2 concentration range of 0%, v/v to 8%, v/v. Furthermore, the sensor possesses the same, unchanged sensor signal even at varied medium temperatures between 210 °C and 270 °C of the gas stream. At flow rates of the gas stream from 8 m3/h to 12 m3/h, the sensor has shown only a slightly reduced sensitivity at a low flow rate of 8 m3/h. The sensor characterisation demonstrates the suitability of the calorimetric gas sensor for monitoring the efficiency of industrial sterilisation processes.
The esophageal Doppler monitor (EDM) is a minimally-invasive hemodynamic device which evaluates both cardiac output (CO), and fluid status, by estimating stroke volume (SV) and calculating heart rate (HR). The measurement of these parameters is based upon a continuous and accurate approximation of distal thoracic aortic blood flow. Furthermore, the peak velocity (PV) and mean acceleration (MA), of aortic blood flow at this anatomic location, are also determined by the EDM. The purpose of this preliminary report is to examine additional clinical hemodynamic calculations of: compliance (C), kinetic energy (KE), force (F), and afterload (TSVRi). These data were derived using both velocity-based measurements, provided by the EDM, as well as other contemporaneous physiologic parameters. Data were obtained from anesthetized patients undergoing surgery or who were in a critical care unit. A graphical inspection of these measurements is presented and discussed with respect to each patient’s clinical situation. When normalized to each of their initial values, F and KE both consistently demonstrated more discriminative power than either PV or MA. The EDM offers additional applications for hemodynamic monitoring. Further research regarding the accuracy, utility, and limitations of these parameters is therefore indicated.
Mit Gesetz vom 29.7.2009 (BGBl 2009 I S. 2355) hat der deutsche Gesetzgeber die EU-Verbraucherkreditrichtlinie 2008/48/EG vom 23.4.2008 NWB PAAAD-28762 in nationales Recht umgesetzt. In diesem Gesetz ist auch der Immobiliardarlehensvertrag neu geregelt worden. Auf diese besondere Kreditform sind grds. die Vorschriften zum Verbraucherkredit anzuwenden, so dass der Darlehensgeber auch im Fall einer Immobilienfinanzierung zu vorvertraglicher Information des Verbrauchers verpflichtet ist. Beim finanzierten Immobilienerwerb werden Veräußerer und kreditgebende Bank häufig in Abstimmung und Zusammenarbeit tätig, so dass für die Bank besondere Aufklärungspflichten gegenüber dem Verbraucher entstehen. Im Fall der Verletzung dieser Pflichten drohen ihr Schadensersatzansprüche oder ein Widerrufsrecht des Verbrauchers.
Mit einer aktuellen Entscheidung hat der BGH, Urt. v. 11.5.2012 - V ZR 196/11, MDR 2012, 958 seine Rechtsprechung v. 5.6.2008 - V ZB 85/07 zur Entstehungsphase einer Wohnungseigentümergemeinschaft fortgeführt. In dieser Entscheidung sieht der BGH die Erwerber von Wohnungseigentum als Kostenschuldner gem. § 16 Abs. 2 WEG an, obwohl diese noch nicht im Grundbuch eingetragen sind. Eine andere Rechtslage besteht beim sog. Zweiterwerb. Die Abgrenzung von Erstund Zweierwerb ist aufgrund der völlig unterschiedlichen Rechtsfolgen für die Praxis von großer Bedeutung.
A variety of transition metals, e.g., copper, zinc, cadmium, lead, etc. are widely used in industry as components for wires, coatings, alloys, batteries, paints and so on. The inevitable presence of transition metals in industrial processes implies the ambition of developing a proper analytical technique for their adequate monitoring. Most of these elements, especially lead and cadmium, are acutely toxic for biological organisms. Quantitative determination of these metals at low activity levels in different environmental and industrial samples is therefore a vital task. A promising approach to achieve an at-side or on-line monitoring on a miniaturized and cost efficient way is the combination of a common potentiometric sensor array with heavy metal-sensitive thin-film materials, like chalcogenide glasses and polymeric materials, respectively.
The chemical imaging sensor is a chemical sensor which is capable of visualizing the spatial distribution of chemical species in sample solution. In this study, a novel measurement system based on the chemical imaging sensor was developed to observe the inside of a Y-shaped microfluidic channel while injecting two sample solutions from two branches. From the collected chemical images, it was clearly observed that the injected solutions formed laminar flows in the microfluidic channel. In addition, ion diffusion across the laminar flows was observed. This label-free method can acquire quantitative data of ion distribution and diffusion in microfluidic devices, which can be used to determine the diffusion coefficients, and therefore, the molecular weights of chemical species in the sample solution.
Wagner, Torsten ; Shigiahara, N. ; Miyamoto, K. ; Suzurikawa, J. ; Finger, F. ; Schöning, Michael Josef ; Yoshinobu, T.
This work describes the novel combination of the light-addressable electrode (LAE) and the light-addressable potentiometric sensor (LAPS) into a microsystem set-up. Both the LAE as well as the LAPS shares the principle of addressing the active spot by means of a light beam. This enables both systems to manipulate resp. to detect an analyte with a high spatial resolution. Hence, combining both principles into a single set-up enables the active stimulation e.g., by means of electrolysis and a simultaneous observation e.g., the response of an entrapped biological cell by detection of extracellular pH changes. The work will describe the principles of both technologies and the necessary steps to integrate them into a single set-up. Furthermore, examples of application and operation of such systems will be presented.