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AgTcO4 reacts with R3ECl compounds (E = C, Si, Ge, Sn, Pb; R = Me, iPr, tBu, Ph), tBu2SnCl2, or PhMgCl under formation of novel trioxotechnetium(VII) derivatives. The carbon and silicon derivatives readily undergo decomposition, which was proven by 99Tc NMR spectroscopy and the isolation of decomposition products such as [TcOCl3(THF)(OH2)]. Compounds [Ph3GeOTcO3], [(THF)Ph3SnOTcO3], [(O3TcO)SntBu2(OH)]2, and [(THF)4Mg(OTcO3)2] are more stable and were isolated in crystalline form and characterized by X-ray diffraction.
This Research Briefing, issued in July 2010, concluded that:
- Small and medium-sized enterprises (SMEs) in Europe have long called for a matching legal form valid across the EU (similar to that of the European company (SE) for large firms)
- The main benefits would be the availability of uniform Europe-wide company structures, significant cost reductions for businesses and further integration of the internal market
- Given the differing national views regarding the concrete features of the new legal form there is currently no sign of an agreement being reached at the European level in the short term; however, it is possible that progress will be made in negotiations during the year
- The key issues being discussed in depth are company formation, transnationality and employee participation rights in the new European private company (SPE).
The potential of electronic markets in enabling innovative product bundles through flexible and sustainable partnerships is not yet fully exploited in the telecommunication industry. One reason is that bundling requires seamless de-assembling and re-assembling of business processes, whilst processes in telecommunication companies are often product-dependent and hard to virtualize. We propose a framework for the planning of the virtualization of processes, intended to assist the decision maker in prioritizing the processes to be virtualized: (a) we transfer the virtualization pre-requisites stated by the Process Virtualization Theory in the context of customer-oriented processes in the telecommunication industry and assess their importance in this context, (b) we derive IT-oriented requirements for the removal of virtualization barriers and highlight their demand on changes at different levels of the organization. We present a first evaluation of our approach in a case study and report on lessons learned and further steps to be performed.
Kurzarbeit war und ist eines der wesentlichen Elemente der Unternehmen, um dem durch die Finanz- und Wirtschaftskrise begründeten Arbeitskräfteüberhang begegnen zu können. Laut statistischer Mitteilung der Bundesagentur für Arbeit waren im Mai 2009 ca. 1,52 Mio. Arbeitnehmer in Kurzarbeit, im März 2010 waren es immer noch 830.000 Arbeitnehmer, die Kurzarbeitergeld bezogen haben. Bis Ende 2010 können Arbeitgeber Kurzarbeit noch für die verlängerte Höchstbezugsdauer von bis zu 18 Monaten beantragen. Zudem hat der Bundestag am 8.7.2010 eine nochmalige Verlängerung der Erstattung der Sozialversicherungsbeiträge bis März 2012 verabschiedet. Trotzdem müssen zahlreiche Arbeitgeber inzwischen feststellen, dass sie allein mit dem Mittel der Kurzarbeit nicht um einen Personalabbau herumkommen. Dies gilt insbesondere für diejenigen Unternehmen, die gleich zu Beginn der Krise im Herbst 2008 Kurzarbeit eingeführt haben und bei denen deshalb die damals noch geltende 24-monatige Höchstbezugsdauer im Herbst 2010 auslaufen wird
Betriebsratswahlen 2010
(2010)
Die nächsten regelmäßigen Wahlen zum Betriebsrat (BR) stehen vor der Tür. Sie finden in der Zeit vom 1.3.2010 bis 31.5.2010 statt. Die Wahlen sind zwar vornehmlich Sache der Arbeitnehmer, allerdings sollten auch die Arbeitgeber darauf achten, dass die Wahlen korrekt ablaufen, schließlich haben die Arbeitgeber die Kosten der Wahlen zu tragen. Kommt es hierbei zu Fehlern, die schlimmstenfalls eine Wiederholung der Wahlen erfordern, hat auch für die hierdurch zusätzlich entstehenden Kosten der Arbeitgeber aufzukommen. Zudem bietet die aktive und unterstützende Begleitung der Wahlen für die Arbeitgeber die Chance, die Grundlagen für eine konstruktive Zusammenarbeit mit dem künftigen Betriebsrat zu legen. Der vorliegende Beitrag erläutert die wesentlichen Abläufe des Wahlverfahrens sowie die hierbei zwingend zu beachtenden Fristen.
Orzada, Stephan ; Maderwald, Stefan ; Poser, Benedikt Andreas ; Bitz, Andreas ; Quick, Harald H. ; Ladd, Mark E.
As the field strength and, therefore, the operational frequency in MRI is increased, the wavelength approaches the size of the human head/body, resulting in wave effects, which cause signal decreases and dropouts. Several multichannel approaches have been proposed to try to tackle these problems, including RF shimming, where each element in an array is driven by its own amplifier and modulated with a certain (constant) amplitude and phase relative to the other elements, and Transmit SENSE, where spatially tailored RF pulses are used. In this article, a relatively inexpensive and easy to use imaging scheme for 7 Tesla imaging is proposed to mitigate signal voids due to B1 field inhomogeneity. Two time-interleaved images are acquired using a different excitation mode for each. By forming virtual receive elements, both images are reconstructed together using GRAPPA to achieve a more homogeneous image, with only small SNR and SAR penalty in head and body imaging at 7 Tesla.
Kraff, Oliver ; Bitz, Andreas ; Dammann, Philipp ; Ladd, Susanne C. ; Ladd, Mark E. ; Quick, Harald H.
Purpose:
MRI plays a leading diagnostic role in assessing the musculoskeletal (MSK) system and is well established for most questions at clinically used field strengths (up to 3 T). However, there are still limitations in imaging early stages of cartilage degeneration, very fine tendons and ligaments, or in locating nerve lesions, for example. 7 T MRI of the knee has already received increasing attention in the current published literature, but there is a strong need to develop new radiofrequency (RF) coils to assess more regions of the MSK system. In this work, an eight-channel transmit/receive RF array was built as a multipurpose coil for imaging some of the thus far neglected regions. An extensive coil characterization protocol and first in vivo results of the human wrist, shoulder, elbow, knee, and ankle imaged at 7 T will be presented.
Methods:
Eight surface loop coils with a dimension ofurn:x-wiley:00942405:media:mp7176:mp7176-math-0001 were machined from FR4 circuit board material. To facilitate easy positioning, two coil clusters, each with four loop elements, were combined to one RF transmit/receive array. An overlapped and shifted arrangement of the coil elements was chosen to reduce the mutual inductance between neighboring coils. A phantom made of body-simulating liquid was used for tuning and matching on the bench. Afterward, the S-parameters were verified on a human wrist, elbow, and shoulder. For safety validation, a detailed compliance test was performed including full wave simulations of the RF field distribution and the corresponding specific absorption rate (SAR) for all joints. In vivo images of four volunteers were assessed with gradient echo and spin echo sequences modified to obtain optimal image contrast, full anatomic coverage, and the highest spatial resolution within a reasonable acquisition time. The performance of the RF coil was additionally evaluated by in vivo B1 mapping.
Results:
A comparison of B1 per unit power, flip angle distribution, and anatomic images showed a fairly homogeneous excitation for the smaller joints (elbow, wrist, and ankle), while for the larger joints, the shoulder and especially the knee, B1 inhomogeneities and limited penetration depth were more pronounced. However, the greater part of the shoulder joint could be imaged.In vivo images rendered very fine anatomic details such as fascicles of the median nerve and the branching of the nerve bundles. High-resolution images of cartilage, labrum, and tendons could be acquired. Additionally, turbo spin echo (TSE) and inversion recovery sequences performed very well.
Conclusions:
This study demonstrates that the concept of two four-channel transmit/receive RF arrays can be used as a multipurpose coil for high-resolutionin vivo MR imaging of the musculoskeletal system at 7 T. Not only gradient echo but also typical clinical and SAR-intensive sequences such as STIR and TSE performed well. Imaging of small structures and peripheral nerves could in particular benefit from this technique.
Schlamann, Marc ; Voigt, Melanie A. ; Maderwald, Stefan ; Bitz, Andreas ; Kraff, Oliver ; Ladd, Susanne C. ; Ladd, Mark E. ; Forsting, Michael ; Wilhelm, Hans
Purpose
To assess potential cognitive deficits under the influence of static magnetic fields at various field strengths some studies already exist. These studies were not focused on attention as the most vulnerable cognitive function. Additionally, mostly no magnetic resonance imaging (MRI) sequences were performed.
Materials and Methods
In all, 25 right-handed men were enrolled in this study. All subjects underwent one MRI examination of 63 minutes at 1.5 T and one at 7 T within an interval of 10 to 30 days. The order of the examinations was randomized. Subjects were referred to six standardized neuropsychological tests strictly focused on attention immediately before and after each MRI examination. Differences in neuropsychological variables between the timepoints before and after each MRI examination were assessed and P-values were calculated
Results
Only six subtests revealed significant differences between pre- and post-MRI. In these tests the subjects achieved better results in post-MRI testing than in pre-MRI testing (P = 0.013–0.032). The other tests revealed no significant results.
Conclusion
The improvement in post-MRI testing is only explicable as a result of learning effects. MRI examinations, even in ultrahigh-field scanners, do not seem to have any persisting influence on the attention networks of human cognition immediately after exposure.
Short term effects of magnetic resonance imaging on excitability of the motor cortex at 1.5T and 7T
(2010)
Schlamann, Marc ; Yoon, Min-Suk ; Maderwald, Stefan ; Pietrzyk, Thomas ; Bitz, Andreas ; Gerwig, Marcus ; Forsting, Michael ; Ladd, Susanne C. ; Ladd, Mark E. ; Kastrup, Oliver
Rationale and Objectives
The increasing spread of high-field and ultra-high-field magnetic resonance imaging (MRI) scanners has encouraged new discussion of the safety aspects of MRI. Few studies have been published on possible cognitive effects of MRI examinations. The aim of this study was to examine whether changes are measurable after MRI examinations at 1.5 and 7 T by means of transcranial magnetic stimulation (TMS).
Materials and Methods
TMS was performed in 12 healthy, right-handed male volunteers. First the individual motor threshold was specified, and then the cortical silent period (SP) was measured. Subsequently, the volunteers were exposed to the 1.5-T MRI scanner for 63 minutes using standard sequences. The MRI examination was immediately followed by another TMS session. Fifteen minutes later, TMS was repeated. Four weeks later, the complete setting was repeated using a 7-T scanner. Control conditions included lying in the 1.5-T scanner for 63 minutes without scanning and lying in a separate room for 63 minutes. TMS was performed in the same way in each case. For statistical analysis, Wilcoxon's rank test was performed.
Results
Immediately after MRI exposure, the SP was highly significantly prolonged in all 12 subjects at 1.5 and 7 T. The motor threshold was significantly increased. Fifteen minutes after the examination, the measured value tended toward normal again. Control conditions revealed no significant differences.
Conclusion
MRI examinations lead to a transient and highly significant alteration in cortical excitability. This effect does not seem to depend on the strength of the static magnetic field.
Das verbraucherschützende Widerrufsrecht ist in die Jahre gekommen. Als Ergebnis der zunehmenden Rechtszersplitterung im europäischen Richtlinienrecht hat sich eine unüberschaubare Bandbreite an Ausgestaltungsformen in den mitgliedstaatlichen Rechtssystemen herausgebildet. Effektivitätseinbußen, Wettbewerbsverzerrungen und ein hohes Maß an Rechtsunsicherheit sind die Folgen. Zurückführen lässt sich die kostenintensive Fragmentierung des Verbrauchervertragsrechts auf die bisherige Regelungspolitik der Mindestharmonisierung, die es den Mitgliedstaaten erlaubt, von dem durch die Richtlinien gesetzten Mindeststandard durch „überschießende Umsetzung” abzuweichen. Der Unionsgesetzgeber versucht dem vermehrt durch einen Prozess der Vollharmonisierung zu begegnen: Neben Reformen der Richtlinie über Verbraucherkredite (VerbrKrRL) sowie der Timesharing-Richtlinie (TimesharingRL), liegt seit dem 8. Oktober 2008 nunmehr auch der Entwurf einer Rahmenrichtlinie über die Rechte der Verbraucher (VRRL-E) vor. Danach sollen die bisherigen Richtlinien über Haustürgeschäfte (HaustürgeschäfteRL), Fernabsatzverträge (FARL), missbräuchliche Klauseln sowie Verbrauchsgüterkäufe vereinigt werden. Für das verbraucherschützende Widerrufsrecht ist damit zum einen die Herausbildung einheitlicher Kernelemente verbunden, zum anderen tritt es in Konkurrenz zu umfassenden Informationspflichten, die ebenfalls den Schutz des Verbrauchers vor dem unüberlegten Abschluss riskanter bzw. nachteiliger Verträge zum Ziel haben.
Angesichts dieser Entwicklung stellt sich im Folgenden die Frage nach der Zukunft des unionsrechtlichen Widerrufsrechts. Von besonderer Bedeutung ist dabei die Klärung des Verhältnisses zu den verbraucherschützenden Informationspflichten. Dazu bedarf es zunächst der Herausarbeitung einheitlicher Kernelemente des Widerrufsrechts im vollharmonisierten Richtlinienrecht (II.) sowie einer Systematisierung der sekundärrechtlichen Informationspflichten im Anschluss eine kritische Gegenüberstellung der Vor- und Nachteile beider Schutzinstrumente erfolgen kann (IV.).
Degering, Christian ; Eggert, Thorsten ; Puls, Michael ; Bongaerts, Johannes ; Evers, Stefan ; Maurer, Karl-Heinz ; Jaeger, Karl-Erich
Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.
Zhubanova, Azhar A. ; Aknazarov, S. K. ; Mansurov, Zulkhair ; Digel, Ilya ; Kozhalakova, A. A. ; Akimbekov, Nuraly S. ; O'Heras, Carlos ; Tazhibayeva, S. ; Artmann, Gerhard ; Temiz Artmann, Aysegül
Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.
Die Erdbebensicherheit von Gebäuden aus Kalksandsteinmauerwerk ist aktuell nach DIN 4149 mit linearen Verfahren nachzuweisen. Dies führt in der praktischen Anwendung zu großen Problemen, da selbst traditionell übliche Grundrisse teilweise nicht mehr ohne zusätzliche Maßnahmen nachweisbar sind. Zur Lösung dieser Problematik wurden von der deutschen Mauerwerksindustrie auf nationaler und europäischer Ebene Forschungsprojekte initiiert, deren Ergebnisse in Form von statisch nichtlinearen Verfahren Eingang in den Nationalen Anhang zur DIN EN 1998-1 [12] gefunden haben. Mit den Verfahren wird die Nachweissituation zukünftig grundlegend verbessert, da mit diesen die Tragwerksreserven wesentlich besser ausgenutzt werden können. Im Folgenden wird die Anwendung der Verfahren am Beispiel einer Reihenhauszeile aus Kalksandsteinmauerwerk demonstriert. Der Nachweis der Reihenhäuser wurde im Rahmen einer Zustimmung im Einzelfall, die vom Bundesverband Kalksandsteinindustrie eV in Hannover koordiniert wurde, durch unabhängige Gutachter und die Bauaufsicht eingehend geprüft und für richtig befunden. Die Durchführung des Nachweises erfolgte auf Grundlage eines an der RWTH Aachen entwickelten neuen Nachweiskonzeptes. Die baupraktische Anwendbarkeit und einfache Nachvollziehbarkeit dieses Konzepts ist durch eine softwaretechnische Umsetzung sichergestellt.
Butenweg, Christoph ; Fäcke, Andreas ; Fehling, Ekkehard ; Gellert, Christoph ; Gierga, Michael ; Meyer, Udo ; Rütschlin, Andreas H. ; Stürz, Jochen
Moderne Bauwerke müssen heute eine hohe energetische Leistungsfähigkeit aufweisen und gleichzeitig alle einwirkenden Lasten sicher abtragen. Dies stellt insbesondere in Erdbebengebieten hohe Anforderungen an die verwendeten Baustoffe. Am baupraktischen Beispiel einer Doppelhaushälfte wird demonstriert, dass die Symbiose aus energieeffizientem und gleichzeitig erdbebensicherem Bauen in der höchsten deutschen Erdbebenzone mit monolithischem Ziegelmauerwerk gut realisierbar ist. Als Ziegelmauerwerk werden für die Außenwände wärmetechnisch optimierte Hochlochziegel verwendet, die sowohl die Anforderungen der Energieeinsparverordnung 2009 als auch die Anforderungen an Mauerwerkbaustoffe nach den aktuellen Erdbebennormen erfüllen. Der Erdbebennachweis der Doppelhaushälfte erfolgt mit einem nichtlinearen Nachweisverfahren, das für eine einfache praktische Anwendung programmtechnisch umgesetzt wurde. Für den Nachweis wurden aus zyklischen Schubwandversuchen ermittelte Last-Verformungskurven verwendet. Das gesamte in Deutschland noch nicht normativ geregelte Nachweiskonzept wurde im Rahmen einer Zustimmung im Einzelfall geprüft und genehmigt.
Akimbekov, Nuraly S. ; Zhubanova, A. A. ; Mansurov, Z. A. ; Digel, Ilya ; Artmann, Gerhard ; Temiz Artmann, Aysegül
On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis.
Die Entwicklungen der Rechtsinformatik und des Informationsrechts zeigen, dass diese Disziplinen aktuell vor der Herausforderung stehen, eine interdisziplinäre Zusammenarbeit zwischen ihnen und anderen Disziplinen zu etablieren. Unterschiedliche Publikationskulturen erschweren die Erreichung dieses Ziels. Forschungsportale stellen themenspezifische, internetbasierte Verzeichnisse dar, die bereits vorhandene Informationen strukturiert zugänglich machen. Sie können die Beziehungen zwischen den Disziplinen fördern, indem sie bereits erzielte Arbeitsergebnisse disziplinenübergreifend bekannt machen und dadurch dazu beitragen, Synergiepotenziale und mögliche Kooperationspartner zu identifizieren.
Horstmann, Marie-Therese ; Bialonski, Stephan ; Noenning, Nina ; Mai, Heinke ; Prusseit, Jens ; Wellmer, Jörg ; Hinrichs, Hermann ; Lehnertz, Klaus
Objective
To investigate whether functional brain networks of epilepsy patients treated with antiepileptic medication differ from networks of healthy controls even during the seizure-free interval.
Methods
We applied different rules to construct binary and weighted networks from EEG and MEG data recorded under a resting-state eyes-open and eyes-closed condition from 21 epilepsy patients and 23 healthy controls. The average shortest path length and the clustering coefficient served as global statistical network characteristics.
Results
Independent on the behavioral condition, epileptic brains exhibited a more regular functional network structure. Similarly, the eyes-closed condition was characterized by a more regular functional network structure in both groups. The amount of network reorganization due to behavioral state changes was similar in both groups. Consistent findings could be achieved for networks derived from EEG but hardly from MEG recordings, and network construction rules had a rather strong impact on our findings.
Conclusions
Despite the locality of the investigated processes epileptic brain networks differ in their global characteristics from non-epileptic brain networks. Further methodological developments are necessary to improve the characterization of disturbed and normal functional networks.
Significance
An increased regularity and a diminished modulation capability appear characteristic of epileptic brain networks.
We consider recent reports on small-world topologies of interaction networks derived from the dynamics of spatially extended systems that are investigated in diverse scientific fields such as neurosciences, geophysics, or meteorology. With numerical simulations that mimic typical experimental situations, we have identified an important constraint when characterizing such networks: indications of a small-world topology can be expected solely due to the spatial sampling of the system along with the commonly used time series analysis based approaches to network characterization.
von Knobelsdorf-Brenkenhoff, Florian ; Frauenrath, Tobias ; Prothmann, Marcel ; Dieringer, Matthias A. ; Hezel, Fabian ; Renz, Wolfgang ; Kretschel, Kerstin ; Niendorf, Thoralf ; Schulz-Menger, Jeanette
Objectives
Interest in cardiovascular magnetic resonance (CMR) at 7 T is motivated by the expected increase in spatial and temporal resolution, but the method is technically challenging. We examined the feasibility of cardiac chamber quantification at 7 T.
Methods
A stack of short axes covering the left ventricle was obtained in nine healthy male volunteers. At 1.5 T, steady-state free precession (SSFP) and fast gradient echo (FGRE) cine imaging with 7 mm slice thickness (STH) were used. At 7 T, FGRE with 7 mm and 4 mm STH were applied. End-diastolic volume, end-systolic volume, ejection fraction and mass were calculated.
Results
All 7 T examinations provided excellent blood/myocardium contrast for all slice directions. No significant difference was found regarding ejection fraction and cardiac volumes between SSFP at 1.5 T and FGRE at 7 T, while volumes obtained from FGRE at 1.5 T were underestimated. Cardiac mass derived from FGRE at 1.5 and 7 T was larger than obtained from SSFP at 1.5 T. Agreement of volumes and mass between SSFP at 1.5 T and FGRE improved for FGRE at 7 T when combined with an STH reduction to 4 mm.
Conclusions
This pilot study demonstrates that cardiac chamber quantification at 7 T using FGRE is feasible and agrees closely with SSFP at 1.5 T.
Dobbertin, Matthias ; Eilmann, Britta ; Bleuler, Peter ; Giuggiola, Arnaud ; Graf Pannatier, Elisabeth ; Landolt, Werner ; Schleppi, Patrick ; Rigling, Andreas
In Valais, Switzerland, Scots pines (Pinus sylvestris L.) are declining, mainly following drought. To assess the impact of drought on tree growth and survival, an irrigation experiment was initiated in 2003 in a mature pine forest, approximately doubling the annual precipitation. Tree crown transparency (lack of foliage) and leaf area index (LAI) were annually assessed. Seven irrigated and six control trees were felled in 2006, and needles, stem discs and branches were taken for growth analysis. Irrigation in 2004 and 2005, both with below-average precipitation, increased needle size, area and mass, stem growth and, with a 1-year delay, shoot length. This led to a relative decrease in tree crown transparency (−14%) and to an increase in stand LAI (+20%). Irrigation increased needle length by 70%, shoot length by 100% and ring width by 120%, regardless of crown transparency. Crown transparency correlated positively with mean needle size, shoot length and ring width and negatively with specific leaf area. Trees with high crown transparency (low growth, short needles) experienced similar increases in needle mass and growth with irrigation than trees with low transparency (high growth, long needles), indicating that seemingly declining trees were able to ‘recover’ when water supply became sufficient. A simple drought index before and during the irrigation explained most of the variation found in the parameters for both irrigated and control trees.
Sisyphus wird Steiff
(2010)
• Most of the edible forest mushrooms are mycorrhizal and depend on carbohydrates produced by the associated trees. Fruiting patterns of these fungi are not yet fully understood since climatic factors alone do not completely explain mushroom occurrence.
• The objective of this study was to retrospectively find out if changing tree growth following an increment thinning has influenced the diversity patterns and productivity of associated forest mushrooms in the fungus reserve La Chanéaz, Switzerland.
• The results reveal a clear temporal relationship between the thinning, the growth reaction of trees and the reaction of the fungal community, especially for the ectomycorrhizal species. The tree-ring width of the formerly suppressed beech trees and the fruit body number increased after thinning, leading to a significantly positive correlation between fruit body numbers and tree-ring width.
• Fruit body production was influenced by previous annual tree growth, the best accordance was found between fruit body production and the tree-ring width two years previously.
• The results support the hypothesis that ectomycorrhizal fruit body production must be linked with the growth of the associated host trees. Moreover, the findings indicate the importance of including mycorrhizal fungi as important players when discussing a tree as a carbon source or sink.
Low emission zones and truck bans, the rising price of diesel and increases in road tolls: all of these factors are putting serious pressure on the transport industry. Commercial vehicle manufacturers and their suppliers are in the process of identifying new solutions to these challenges as part of their efforts to meet the EEV (enhanced environmentally friendly vehicle) limits, which are currently the most robust European exhaust and emissions standards for trucks and buses.
Der folgende Bericht fasst Erfahrungen zusammen, die in großen Entwicklungsprojekten der Firma Ericsson über mehrere Jahre gesammelt wurden. Ziel war dabei nicht, agile Methoden und Techniken einzusetzen - Agilität war zu der Zeit noch kein Hype-Thema. Vielmehr wurden Schwächen in den eigenen Projekten identifiziert und verbessert. Der Erfahrungsbericht vergleicht Verbesserungen in diesen Projekten mit den Ansätzen der agilen Entwicklung. Als Ergebnis werden folgende Punkte festgehalten: Erstens, einige Praktiken und Werte der agilen Entwicklung lassen sich auch in Großprojekten einsetzen. Zweitens, bei der Skalierung für Großprojekte werden diese Praktiken langsamer getaktet. Drittens, agile Entwicklung ist nicht nur eine Reihe von Praktiken und Werten, agile Entwicklung ist vielmehr auch eine Frage der Entwicklungs- und Projektkultur. Diese kulturelle Änderung lässt sich in Großprojekten deutlich langsamer umsetzen.
Recently, we introduced and mathematically analysed a new method for grid deformation (Grajewski et al., 2009) [15] we call basic deformation method (BDM) here. It generalises the method proposed by Liao et al. (Bochev et al., 1996; Cai et al., 2004; Liao and Anderson, 1992) [4], [6], [20]. In this article, we employ the BDM as core of a new multilevel deformation method (MDM) which leads to vast improvements regarding robustness, accuracy and speed. We achieve this by splitting up the deformation process in a sequence of easier subproblems and by exploiting grid hierarchy. Being of optimal asymptotic complexity, we experience speed-ups up to a factor of 15 in our test cases compared to the BDM. This gives our MDM the potential for tackling large grids and time-dependent problems, where possibly the grid must be dynamically deformed once per time step according to the user's needs. Moreover, we elaborate on implementational aspects, in particular efficient grid searching, which is a key ingredient of the BDM.
The continuously growing amount of renewable sources starts compromising the stability of electrical grids. Contradictory to fossil fuel power plants, energy production of wind and photovoltaic (PV) energy is fluctuating. Although predictions have significantly improved, an outage of multi-MW offshore wind farms poses a challenging problem. One solution could be the integration of storage systems in the grid. After a short overview, this paper focuses on two exemplary battery storage systems, including the required power electronics. The grid integration, as well as the optimal usage of volatile energy reserves, is presented for a 5- kW PV system for home application, as well as for a 100- MW medium-voltage system, intended for wind farm usage. The efficiency and cost of topologies are investigated as a key parameter for large-scale integration of renewable power at medium- and low-voltage.
Fast response of Scots pine to improved water availability reflected in tree-ring width and δ13C
(2010)
Eilmann, Britta ; Buchmann, Nina ; Siegwolf, Rolf ; Saurer, Matthias ; Cherubini, Paolo ; Rigling, Andreas
Drought-induced forest decline, like the Scots pine mortality in inner-Alpine valleys, will gain in importance as the frequency and severity of drought events are expected to increase. To understand how chronic drought affects tree growth and tree-ring δ13C values, we studied mature Scots pine in an irrigation experiment in an inner-Alpine valley. Tree growth and isotope analyses were carried out at the annual and seasonal scale. At the seasonal scale, maximum δ13C values were measured after the hottest and driest period of the year, and were associated with decreasing growth rates. Inter-annual δ13C values in early- and latewood showed a strong correlation with annual climatic conditions and an immediate decrease as a response to irrigation. This indicates a tight coupling between wood formation and the freshly produced assimilates for trees exposed to chronic drought. This rapid appearance of the isotopic signal is a strong indication for an immediate and direct transfer of newly synthesized assimilates for biomass production. The fast appearance and the distinct isotopic signal suggest a low availability of old stored carbohydrates. If this was a sign for C-storage depletion, an increasing mortality could be expected when stressors increase the need for carbohydrate for defence, repair or regeneration.
Studying global change through investigation of the plastic responses of xylem anatomy in tree rings
(2010)
Fonti, Patrick ; von Arx, Georg ; García-González, Ignacio ; Eilmann, Britta ; Sass-Klaassen, Ute G. W. ; Gärtner, Holger ; Eckstein, Dieter
Variability in xylem anatomy is of interest to plant scientists because of the role water transport plays in plant performance and survival. Insights into plant adjustments to changing environmental conditions have mainly been obtained through structural and functional comparative studies between taxa or within taxa on contrasting sites or along environmental gradients. Yet, a gap exists regarding the study of hydraulic adjustments in response to environmental changes over the lifetimes of plants. In trees, dated tree-ring series are often exploited to reconstruct dynamics in ecological conditions, and recent work in which wood-anatomical variables have been used in dendrochronology has produced promising results. Environmental signals identified in water-conducting cells carry novel information reflecting changes in regional conditions and are mostly related to short, sub-annual intervals. Although the idea of investigating environmental signals through wood anatomical time series goes back to the 1960s, it is only recently that low-cost computerized image-analysis systems have enabled increased scientific output in this field. We believe that the study of tree-ring anatomy is emerging as a promising approach in tree biology and climate change research, particularly if complemented by physiological and ecological studies. This contribution presents the rationale, the potential, and the methodological challenges of this innovative approach.
Self metathesis of oleochemicals offers a variety of bifunctional compounds, that can be used as monomer for polymer production. Many precursors are in huge scales available, like oleic acid ester (biodiesel), oleyl alcohol (tensides), oleyl amines (tensides, lubricants). We show several ways to produce and separate and purify C18-α,ω-bifunctional compounds, using Grubbs 2nd Generation catalysts, starting from technical grade educts.
Frauenrath, Tobias ; Hezel, Fabian ; Renz, Wolfgang ; de Geyer d'Orth, Thibaut ; Dieringer, Matthias ; von Knobelsdorf-Brenkenhoff, Florian ; Prothmann, Marcel ; Schulz-Menger, Jeanette ; Niendorf, Thoralf
Background
To demonstrate the applicability of acoustic cardiac triggering (ACT) for imaging of the heart at ultrahigh magnetic fields (7.0 T) by comparing phonocardiogram, conventional vector electrocardiogram (ECG) and traditional pulse oximetry (POX) triggered 2D CINE acquisitions together with (i) a qualitative image quality analysis, (ii) an assessment of the left ventricular function parameter and (iii) an examination of trigger reliability and trigger detection variance derived from the signal waveforms.
Results
ECG was susceptible to severe distortions at 7.0 T. POX and ACT provided waveforms free of interferences from electromagnetic fields or from magneto-hydrodynamic effects. Frequent R-wave mis-registration occurred in ECG-triggered acquisitions with a failure rate of up to 30% resulting in cardiac motion induced artifacts. ACT and POX triggering produced images free of cardiac motion artefacts. ECG showed a severe jitter in the R-wave detection. POX also showed a trigger jitter of approximately Δt = 72 ms which is equivalent to two cardiac phases. ACT showed a jitter of approximately Δt = 5 ms only. ECG waveforms revealed a standard deviation for the cardiac trigger offset larger than that observed for ACT or POX waveforms.
Image quality assessment showed that ACT substantially improved image quality as compared to ECG (image quality score at end-diastole: ECG = 1.7 ± 0.5, ACT = 2.4 ± 0.5, p = 0.04) while the comparison between ECG vs. POX gated acquisitions showed no significant differences in image quality (image quality score: ECG = 1.7 ± 0.5, POX = 2.0 ± 0.5, p = 0.34).
Conclusions
The applicability of acoustic triggering for cardiac CINE imaging at 7.0 T was demonstrated. ACT's trigger reliability and fidelity are superior to that of ECG and POX. ACT promises to be beneficial for cardiovascular magnetic resonance at ultra-high field strengths including 7.0 T.
Barbazán, Paula ; Hagenbach, Adelheid ; Paulßen, Elisabeth ; Abram, Ulrich ; Carballo, Rosa ; Rodriguez-Hermida, Sabina ; Vázquez-López, Ezequiel M.
Tricarbonylrhenium(I) and -technetium(I) halide (halide = Cl and Br) complexes of ligands derived from 4,5-diazafluoren-9-one (df) and 1,10-phenanthroline-5,6-dione (phen) derivatives of benzoic and 2-hydroxybenzoic acid hydrazides have been prepared. The complexes have been characterized by elemental analysis, MS, IR, 1H NMR and absorption and emission UV/Vis spectroscopic methods. The metal centres (ReI and TcI) are coordinated through the nitrogen imine atoms and establish five-membered chelate rings, whereas the hydrazone groups stand uncoordinated. The 1H NMR spectra suggest the same behaviour in solution on the basis of only marginal variations in the chemical shifts of the hydrazine protons.
Ein viel versprechender erneuerbarer Rohstoff für die Produktion von Chemikalien und Treibstoffen ist Lignocellulose aus pflanzlicher Biomasse. Die darin enthaltenen Zucker können mittels enzymatischer Hydrolyse freigesetzt und fermentativ zu Ethanol umgesetzt werden. Ein interessanter Ansatz ist dabei die simultane Verzuckerung und Fermentation. Hefen und Enzyme haben mit 30 °C bzw. 50 °C zwar unterschiedliche Temperaturoptima, es konnte aber gezeigt werden, dass auch bei den niedrigeren Temperaturen eine Umsetzung der Cellulose zu Glucose erfolgt, wenn auch langsamer als bei optimalen Bedingungen. Außerdem konnte in Vorversuchen gezeigt werden, dass Ethanol in den zu erwartenden Konzentrationen keinen Einfluss auf die enzymatische Umsetzung hat.
Sieker, Tim ; Neuner, Andreas ; Dimitrova, Darina ; Tippkötter, Nils ; Bart, Hans-Jörg ; Heinzle, Elmar ; Ulber, Roland
Grassilage stellt einen nachwachsenden Rohstoff mit großem Potenzial dar. Neben Cellulose und Hemicellulose enthält sie auch organische Säuren, insbesondere Milchsäure. In einem Bioraffinerie-Projekt wird die Milchsäure aus der Silage isoliert und mit gentechnisch optimierten Stämmen zu L-Lysin weiterverarbeitet. Die Lignocellulose wird hydrolysiert und zu Ethanol fermentiert. Ein besonderes Augenmerk liegt auf der Integration der unterschiedlichen Prozesse sowie der einzelnen Prozessschritte zu einem Gesamtprozess, der sämtliche Inhaltsstoffe der Silage verwertet.
In den letzten drei bis vier Jahren unterlag der Bereich der Bilanzierung und Offenlegung von unsicheren Steuerpositionen im IFRS- und US-GAAP-Abschluss einem stetigen Wandel. Sowohl nach US-GAAP als auch nach IFRS nehmen die Anforderungen an die Bilanzierung und Offenlegung beständig zu. Schon allein dies zeigt die Bedeutung, die dem Themenbereich der Steuerrisiken beigemessen wird. Aber nicht nur im Rahmen der Berichterstattung im Jahresabschluss stehen Steuerrisiken oben auf der Agenda, auch der amerikanische Internal Revenue Service (IRS) verfolgt das Thema sehr konsequent und plant, bestimmte Unternehmen zu Angaben von Steuerrisiken im Rahmen der Steuererklärungen zu verpflichten.
Der Aufsatz befasst sich mit den neueren Entwicklungen im Bereich der Bilanzierung und Offenlegung von unsicheren Steuerpositionen in der Rechnungslegung nach IFRS, US-GAAP und HGB und der Offenlegung von Steuerrisiken im Rahmen der Steuererklärung.
We analyze the influence of dipole-dipole interactions in an electromagnetically induced transparency set up for a density at the onset of cooperative effects. To this end, we include mean-field models for the influence of local-field corrections and radiation trapping into our calculation. We show both analytically and numerically that the polarization contribution to the local field strongly modulates the phase of a weak pulse. We give an intuitive explanation for this local-field-induced phase modulation and demonstrate that it distinctively differs from the nonlinear self-phase-modulation that a strong pulse experiences in a Kerr medium.
Kozhalakova, A. A. ; Zhubanova, Azhar A. ; Mansurov, Z. A. ; Digel, Ilya ; Tazhibayeva, S. M. ; Artmann, Gerhard ; Temiz Artmann, Aysegül
In this extended abstract we describe the robot programming and planning language READYLOG, a GOLOG dialect which was developed to support the decision making of robots acting in dynamic real-time domains like robotic soccer. The formal framework of READYLOG, which is based on the situation calculus, features imperative control structures like loops and procedures, allows for decision-theoretic planning, and accounts for a continuously changing world. We developed high-level controllers in READYLOG for our soccer robots in RoboCup’s Middle-size league, but also for service robots and for autonomous agents in interactive computer games.
Leimena, W. ; Artmann, Gerhard ; Dachwald, Bernd ; Temiz Artmann, Aysegül ; Gossmann, Matthias ; Digel, Ilya
Autonomous robotic systems for penetrating thick ice shells with simultaneous collecting of scientific data are very promising devices in both terrestrial (glacier, climate research) and extra-terrestrial applications. Technical challenges in development of such systems are numerous and include 3D-navigation, an appropriate energy source, motion control, etc. Not less important is the problem of forward contamination of the pristine glacial environments with microorganisms and biomolecules from the surface of the probe. This study was devoted to establishing a laboratory model for microbial contamination of a newly constructed ice-melting probe called IceMole and to analyse the viability and amount of the contaminating microorganisms as a function of distance. The used bacterial strains were Bacillus subtilis (ATCC 6051) and Escherichia coli (ATCC 11775). The main objective was development of an efficient and reliable in-situ decontamination method of the melting probe. Therefore, several chemical substances were tested in respect of their efficacy to eliminate bacteria on the surface of the melting probe at low temperature (0 - 5 °C) and at continuous dilution by melted water. Our study has shown that at least 99.9% decontamination of the IceMole can be successfully achieved by the injection of 30% (v/v) hydrogen peroxide and 3% (v/v) sodium hypochlorite into the drilling site. We were able to reproduce this result in both time-dependent and depth-dependent experiments. The sufficient amount of 30% (v/v) H₂O₂ or 3% (v/v) NaClO has been found to be approximately 18 L per cm² of the probe’s surface.
Hybrid control for autonomous systems — Integrating learning, deliberation and reactive control
(2010)
The cost of solar tower power plants is dominated by the heliostat field making up roughly 50% of investment costs. Classical heliostat design is dominated by mirrors brought into position by steel structures and drives that guarantee high accuracies under wind loads and thermal stress situations. A large fraction of costs is caused by the stiffness requirements of the steel structure, typically resulting in ~ 20 kg/m² steel per mirror area. The typical cost figure of heliostats (figure mentioned by Solucar at Solar Paces Conference, Seville, 2006) is currently in the area of 150 €/m² caused by the increasing price of the necessary raw materials. An interesting option to reduce costs lies in a heliostat design where all moving parts are protected from wind loads. In this way, drives and mechanical layout may be kept less robust, thereby reducing material input and costs. In order to keep the heliostat at an appropriate size, small mirrors (around 10x10 cm²) have to be used, which are placed in a box with a transparent cover. Innovative drive systems are developed in order to obtain a cost-effective design. A 0,5x0,5 m² demonstration unit will be constructed. Tests of the unit are carried out with a high-precision artificial sun unit that imitates the sun’s path with an accuracy of less than 0.5 mrad and creates a beam of parallel light with a divergence of less than 4 mrad.
Chalcogenide glass materials as membranes for potentiometric sensors for chemical analysis in solutions have been studied since more than 20 years. In this work, an electrolyte–insulator–semiconductor structure was combined with chalcogenide glass membranes prepared by means of the pulsed laser deposition technique. Depending on the membrane composition a selectivity to different ions (Cd2+ and Pb2+) is achieved. The different sensor membranes have been physically characterised using microscopy, ellipsometry, profilometry, atomic force microscopy (AFM), scanning electron microscopy (SEM) and Rutherford backscattering spectrometry (RBS). The electrochemical behaviour has been investigated via capacitance/voltage (C/V) and constant capacitance (ConCap) measurements and results in a Cd2+ sensitivity of 23.1 ± 0.6 mV per decade in a linear range from 7 × 10−6 to 10−2 mol/l and 24.4 ± 0.5 mV per decade in a linear range from 5 × 10−6 to 10−2 mol/l for Pb2+, respectively.
Helle Fensterprofilmaterialien : Alterungsverhalten auf Basis von peroxidisch vernetztem EPDM
(2010)
Leon Battista Alberti, probably the most innovative architect of early Renaissance Italy, has always fascinated scholars by virtue of the striking interpenetration of theory and practice manifest in his work. As an architect, Alberti was an autodidact. Without the benefit of the formative influence of a master or design education, the roots of his conception of architecture lie in his intellectual formation through humanistic rhetoric. The present study demonstrates with reference to a specific project — the Tempietto of the Holy Sepulchre in Florence — how Alberti’s humanist approach conditioned his method of architectural design.
Umweltzonen und Fahrverbote, kletternde Dieselpreise und steigende Straßenbenutzungsgebühren setzen dem Transportgewerbe aktuell schwer zu. Nutzfahrzeughersteller und Partner finden auf diese Herausforderungen neue Antworten mit der Erfüllung der EEV-Grenzwerte (Enhanced Environmentally Friendly Vehicle) als den gegenwärtig anspruchsvollsten europäischen Abgasstandard für Lkw und Busse.
The Newtonian regime of a recent nonlocal extension of general relativity is investigated. Nonlocality is introduced via a scalar “constitutive” kernel in a special case of the translational gauge theory of gravitation, namely, the teleparallel equivalent of general relativity. In this theory, the nonlocal aspect of gravity simulates dark matter. A nonlocal and nonlinear generalization of Poisson’s equation of Newtonian gravitation is presented. The implications of nonlocality for the gravitational physics in the solar system are briefly studied.
Der BGH hat entschieden, dass ein Vermieter einen Wohnraummietvertrag nicht deshalb kündigen kann, weil der Mieter die Prozesskosten eines früheren, auf Zahlungsverzug gestützten Räumungsprozesses nicht begleicht. Der folgende Beitrag beleuchtet die Konsequenzen dieser Entscheidung für die mietvertragliche Praxis
Wagner, Torsten ; Werner, Frederik ; Miyamoto, Ko-ichiro ; Ackermann, Hans-Josef ; Yoshinobu, Tatsuo ; Schöning, Michael Josef
The development of new interfaces for (bio-)chemical sensors requires comprehensive analyses and testing. The light-addressable potentiometric sensor (LAPS) can be used as a platform to investigate the sensitivity of a newly developed interface towards (bio-)chemical agents. LAPS measurements are spatially resolved by utilisation of focused light beams to define individual measurement spots. In this work, a new digitally modulated LAPS set-up based on an FPGA design will be introduced to increase the number of measurement spots, to shorten the measurement time and to improve the measurement accuracy.
This paper develops an investment/pricing model for the deployment of basic broadband networks which, along with other applications, is applicable to public–private partnership projects. In particular, a new investment model is suggested to be used for finance deployment over a longer term by enabling both private and public investors to participate in the roll-out of next generation access (NGA) infrastructure. This so-called “long-term risk sharing concept” has several notable benefits compared with the traditional regulatory approach. Above all, the model enables both private operators and public authorities to share the risk of investing in NGA infrastructure. Thus the model offers a way for public authorities to achieve a timely and countrywide roll-out of NGA networks, including in areas where NGA investment would otherwise not occur.
Glasfaserbasierte Breitbandnetze werden in Deutschland auch aufgrund der Wirtschaftskrise nur sehr zögerlich ausgebaut. Markus Fredebeul-Krein hält eine staatliche Subventionierung der Breitbandnetze nur bedingt für gerechtfertigt. Private Investoren benötigen jedoch dringend Planungssicherheit durch verlässliche Regulierungsvorschriften.
A novel scheme for precise diagnostics and effective stabilization of currents in a fuel cell stack
(2010)
A novel scheme for detecting inhomogeneous internal currents in a fuel cell stack is presented. In this paper the scheme is investigated for the case that the flow field plates consist of graphite. Then plates of high conductivity, e.g. aluminium between the flow field plates together with small slits in these plates have three effects: (a) Whenever a local inhomogeneity of the electric current occurs at a particular cell in the stack, this will induce a surface current close to that cell perpendicular to the averaged current. This current can be detected. (b) The plates of high conductivity completely prevent the inhomogeneities from spreading to neighbouring cells. (c) Even at the particular cell the inhomogeneity is suppressed as far as possible. Thus this scheme leads to much better diagnostic possibilities and at the same time reduces electric instabilities to an extent, where they probably become harmless. This scheme will first be explained for a simple model to clarify the idea. However, very precise three dimensional computations using realistic parameters are presented, corroborating the results of the simple model.
The mission of the COMPASS-1 picosatellite is to take pictures of the earth, to validate a space-borne GPS receiver developed by the German Aerospace Center, and to verify the proper operation of the magnetic attitude control system in orbit. The spacecraft was launched on April 28, 2008 from the Indian space port Sriharikota, as part of the PSLV-C9 world record launch that simultaneously brought ten satellites into orbit. The mission operations were carried out from the ground stations in Aachen and Tainan. Arising difficulties in the communication link were overcome with the support of individuals from the amateur radio community. After several months of mission operation, abundant housekeeping and mission data has been commanded, received and analyzed and is presented in this paper.
A novel tomographic scheme for analysing the state of any single membrane electrode assembly (MEA) in a stack is suggested. Plates of very high conductivity placed between every fuel cell and slitted in an appropriate manner cause surface currents at well-defined locations of the stack. We show that knowing these surface currents, information about anomalies of the currents in a MEA can be obtained using the methods of tomography. The results are mathematically not unique. However, when assuming plausible defect structures, one can exclude improbable deficiencies by applying a special form of simulated annealing. We present numerical calculations of typical examples demonstrating that the essential defects of the MEA in any single cell of the stack can be detected and their extent can be determined.
Durch rapide gesellschaftliche Veränderungen und neue Anforderungen auf dem Arbeitsmarkt gewinnt die akademische berufliche Weiterbildung zunehmend an Bedeutung. Die Nachfrage nach entsprechenden Angeboten ist sowohl seitens der Unternehmen als auch der Arbeitnehmer/-innen hoch. Die Hochschulen sind aufgefordert, darauf zu reagieren und Wege für lebenslanges Lernen aufzuzeigen, indem sie außerhalb der Hochschule erworbene Kenntnisse auf Hochschulabschlüsse anerkennen und Möglichkeiten schaffen, diese Kenntnisse mit dem Ziel weiterzuentwickeln, einen akademischen Abschluss zu erreichen. Dieser Beitrag stellt einen Studiengang vor, der es Berufstätigen ermöglicht, durch die Anrechnung beruflich erworbener Kompetenzen bis zu 50 Prozent der zu erbringenden Studienleistung abzugelten.
Comparison of intravenous immunoglobulins for naturally occurring autoantibodies against amyloid-β
(2010)
Balakrishnan, Karthikeyan ; Andrei-Selmer, Luminita-Cornelia ; Selmer, Thorsten ; Bacher, Michael ; Dodel, Richard
Intravenous immunoglobulins (IVIG) are currently used for therapeutic purposes in autoimmune disorders. Recently, we demonstrated the presence of naturally occurring antibodies against amyloid- β (nAbs-Aβ) within the pool of IVIG. In this study, we compared different brands of IVIG for nAbs-Aβ and have found differences in the specificity of the nAbs-Aβ towards Aβ1–40 and Aβ1–42 . We analyzed the influence of a pH-shift over the course of antibody storage using ELISA and investigated antibody dimerization at acidic and neutral pH as well as differences in the IgG subclass distributions among the IVIG using both HPLC and a nephelometric assay. Furthermore, we investigated the epitope region of purified nAbs-Aβ. The differences found in Aβ specificity are not directly proportionate to the binding nature of these antibodies when administered in vivo. This information, however, may serve as a guide when choosing the commercial source of IVIG for therapeutic applications in Alzheimer's disease
Since the 80s power production with solar thermal power plants has been a way to substitute fossil fuels. By concentrating direct solar radiation from heliostats very high temperatures of a thermal fluid can be reached. The resulting heat is converted to mechanical energy in a steam cycle which generates electricity.
High efficiencies and fast start-up are reached by using air as a heat medium, as well as using porous ceramic materials as solar receiver of the concentrated sunlight.
In Germany the construction of a 1.5 MWe solar tower power plant began in 2008. It is operational since December 2008 and started production of electricity in the spring of 2009.
In Greece and Cyprus, countries with high solar potential, the development of this competitive solar thermal technology is imperative, since it has already been implemented in other Mediterranean countries.
Bäcker, Matthias ; Poghossian, Arshak ; Abouzar, Maryam H. ; Wenmackers, Sylvia ; Janssens, Stoffel D. ; Haenen, Ken ; Wagner, Patrick ; Schöning, Michael Josef
Capacitive field-effect electrolyte-diamond-insulator-semiconductor (EDIS) structures with O-terminated nanocrystalline diamond (NCD) as sensitive gate material have been realized and investigated for the detection of pH, penicillin concentration, and layer-by-layer adsorption of polyelectrolytes. The surface oxidizing procedure of NCD thin films as well as the seeding and NCD growth process on a Si-SiO2 substrate have been improved to provide high pH-sensitive, non-porous thin films without damage of the underlying SiO2 layer and with a high coverage of O-terminated sites. The NCD surface topography, roughness, and coverage of the surface groups have been characterized by SEM, AFM and XPS methods. The EDIS sensors with O-terminated NCD film treated in oxidizing boiling mixture for 45 min show a pH sensitivity of about 50 mV/pH. The pH-sensitive properties of the NCD have been used to develop an EDIS-based penicillin biosensor with high sensitivity (65-70 mV/decade in the concentration range of 0.25-2.5 mM penicillin G) and low detection limit (5 μM). The results of label-free electrical detection of layer-by-layer adsorption of charged polyelectrolytes are presented, too.
Beging, Stefan ; Mlynek, Daniela ; Hataihimakul, Sudkanung ; Poghossian, Arshak ; Baldsiefen, Gerhard ; Busch, Heinz ; Laube, Norbert ; Kleinen, Lisa ; Schöning, Michael Josef
Urinary stone formation has been evolved to a widespread disease during the last years. The reason for the formation of urinary stones are little crystals, mostly composed of calcium oxalate, which are formed in human kidneys. The early diagnosis of the risk for urinary stone formation of patients can be determined by the “Bonn-Risk-Index” method based on the potentiometric detection of the Ca2+-ion concentration and an optical determination of the triggered crystallisation of calcium oxalate in unprocessed urine. In this work, miniaturised capacitive field-effect EMIS (electrolyte-membrane-insulator-semiconductor) sensors have been developed for the determination of the Ca2+-ion concentration in human native urine. The Ca2+-sensitive EMIS sensors have been systematically characterised by impedance spectroscopy, capacitance–voltage and constant–capacitance method in terms of sensitivity, signal stability and response time in both CaCl2 solutions and in native urine. The obtained results demonstrate the suitability of EMIS sensors for the measurement of the Ca2+-ion concentration in native urine of patients.
Reisert, Steffen ; Henkel, Hartmut ; Schneider, Andreas ; Schäfer, Daniel ; Friedrich, Peter ; Berger, Jörg ; Schöning, Michael Josef
A handheld sensor system for the online measurement of hydrogen peroxide (H2O2) in aseptic sterilisation processes has been developed. It is based on a calorimetric-type gas sensor that consists of a differential set-up of two temperature sensors, of which one is catalytically activated and the second one is passivated and used as reference. The sensor principle relies in detecting a rise in temperature on the active sensor due to the exothermic reaction of H2O2 on the catalytic surface. To characterise the sensor system towards H2O2 sensitivity and other influencing factors, measurements have been carried out both at an experimental set-up and a manufacturer's sterilisation machine. Physical sensor characterisation was done by means of the optical microscopy.
Miyamoto, Ko-ichiro ; Sugawara, Yuri ; Kanoh, Shin´ichiro ; Yoshinobu, Tatsuo ; Wagner, Torsten ; Schöning, Michael Josef
The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the spatial distribution of chemical species. For the practical application of this sensor, artifacts in the chemical images due to defects of the semiconductor substrate and contamination of the sensing surface etc. have been a major problem. An image correction method was developed to eliminate the influence of nonuniformity of individual sensor plate.
Abouzar, Maryam H. ; Poghossian, Arshak ; Siqueira, José R. Jr. ; Oliveira, Osvaldo N. Jr. ; Moritz, Werner ; Schöning, Michael Josef
A novel strategy for enhanced field-effect biosensing using capacitive electrolyte–insulator–semiconductor (EIS) structures functionalised with pH-responsive weak polyelectrolyte/enzyme or dendrimer/enzyme multilayers is presented. The feasibility of the proposed approach is exemplarily demonstrated by realising a penicillin biosensor based on a capacitive p-Si–SiO2 EIS structure functionalised with a poly(allylamine hydrochloride) (PAH)/penicillinase and a poly(amidoamine) dendrimer/penicillinase multilayer. The developed sensors response to changes in both the local pH value near the gate surface and the charge of macromolecules induced via enzymatic reaction, resulting in a higher sensitivity. For comparison, an EIS penicillin biosensor with adsorptively immobilised penicillinase has been also studied. The highest penicillin sensitivity of 100 mV/dec has been observed for the EIS sensor functionalised with the PAH/penicillinase multilayer. The lower and upper detection limit was around 20 µM and 10 mM, respectively. In addition, an incorporation of enzymes in a multilayer prepared by layer-by-layer technique provides a larger amount of immobilised enzymes per sensor area, reduces enzyme leaching effects and thus, enhances the biosensor lifetime (the loss of penicillin sensitivity after 2 months was 10–12%).
Gas sensor investigation based on a catalytically activated thin-film thermopile for H2O2 detection
(2010)
Schöning, Michael Josef ; Kirchner, Patrick ; Ng, Yue Ann ; Spelthahn, Heiko ; Schneider, Andreas ; Henkel, Hartmut ; Friedrich, Peter ; Kolstad, Jens ; Berger, Jörg ; Keusgen, Michael
In aseptic filling systems, hydrogen peroxide vapour is commonly used for the reduction of microbial contaminations in carton packages. In this process, the germicidal efficiency of the vapour depends especially on the H₂O₂ concentration. To monitor the H₂O₂ concentration, a calorimetric H₂O₂ gas sensor based on a catalytically activated thin-film thermopile is investigated. Two different sensor layouts, namely a circular and a linear form, as well as two various material pairs such as tungsten/nickel and gold/nickel, have been examined for the realization of a thin-film thermopile. Additionally, manganese oxide and palladium particles have been compared as responsive catalysts towards H₂O₂. The thin-film sensors have been investigated at various H₂O₂ concentrations, gas temperatures and flow rates.
Buniatyan, Vahe V. ; Abouzar, Maryam H. ; Martirosyan, Norayr W. ; Schubert, Jürgen ; Gevorgian, Spartak ; Schöning, Michael Josef ; Poghossian, Arshak
pH-sensitive properties of barium strontium titanate (BST) high-k thin films as alternative gate material for field-effect capacitive (bio-)chemical sensors based on an electrolyte-insulator-semiconductor system have been investigated. The BST films of different compositions (Ba0.31Sr0.69TiO3, Ba0.25Sr0.75TiO3 and Mg-doped Ba0.8Sr0.2Mg0.1Ti0.9O3) were deposited by pulsed laser deposition technique from targets fabricated by self-propagating high-temperature synthesis. The realised sensors have been electrochemically characterised by means of impedance-spectroscopy, capacitance–voltage and constant-capacitance method. The sensors possess a Nernstian-like pH sensitivity in the concentration range between pH 3 and 11 with a response time of 5–10 s. An equivalent circuit model for the BST-based capacitive field-effect sensor is discussed.
Simultaneous detection of cyanide and heavy metals for environmental analysis by means of µISEs
(2010)
Turek, Monik ; Heiden, Wolfgang ; Guo, Sharon ; Riesen, Alfred ; Schubert, Jürgen ; Zander, Willi ; Krüger, Peter ; Keusgen, Michael ; Schöning, Michael Josef
In environmental analysis, cyanide and heavy metals play an important role, because these substances are highly toxic for biological systems. They can lead to chronic and acute diseases. Due to the chemical properties of cyanide it is frequently used for industrial processes such as extraction of silver and gold. Heavy metals can be found as trace elements in nature and are often applied in industries e.g., galvanization processes. Up to now, cyanide and heavy metals can be detected by several sensors separately and their detection is often limited to laboratory investigations. In this publication, with regard to an in situ analysis, a new miniaturized silicon-based sensor system for the simultaneous detection of cyanide and heavy metals in aqueous solutions is presented that is based on chalcogenide glass-based micro ion-selective electrodes (µISEs). The µISEs are incorporated into a specially designed measuring system for the simultaneous detection of heavy metals and cyanide in solutions and validated by simultaneous measurements of Cu2+- and CN−-ions, Cd2+- and CN−- ions and Pb2+- and CN−-ions. The particular sensor system has shown good sensor properties in the µ-molar ion-concentration range. For simultaneous measurements in complex heavy metal and cyanide solutions an intelligent software using fuzzy logic is discussed.
Jansen, S. V. ; Behbahani, Mehdi ; Laumen, M. ; Kaufmann, T. ; Hormes, M. ; Behr, Marek ; Schmitz-Rode, T. ; Steinseifer, U.
Behbahani, Mehdi ; Probst, M. ; Mai, A. ; Tran, L. ; Vonderstein, K. ; Keschenau, P. ; Linde, T. ; Steinseifer, U. ; Behr, Marek ; Mottaghy, K.
Siqueira, José R. Jr. ; Bäcker, Matthias ; Poghossian, Arshak ; Zucolotto, Valtencir ; Oliveira, Osvaldo N. Jr. ; Schöning, Michael Josef
The control of molecular architecture provided by the layer-by-layer (LbL) technique has led to enhanced biosensors, in which advantageous features of distinct materials can be combined. Full optimization of biosensing performance, however, is only reached if the film morphology is suitable for the principle of detection of a specific biosensor. In this paper, we report a detailed morphology analysis of LbL films made with alternating layers of single-walled carbon nanotubes (SWNTs) and polyamidoamine (PAMAM) dendrimers, which were then covered with a layer of penicillinase (PEN). An optimized performance to detect penicillin G was obtained with 6-bilayer SWNT/PAMAM LbL films deposited on p-Si-SiO2-Ta2O5 chips, used in biosensors based on a capacitive electrolyte-insulator-semiconductor (EIS) and a light-addressable potentiometric sensor (LAPS) structure, respectively. Field-emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM) images indicated that the LbL films were porous, with a large surface area due to interconnection of SWNT into PAMAM layers. This morphology was instrumental for the adsorption of a larger quantity of PEN, with the resulting LbL film being highly stable. The experiments to detect penicillin were performed with constant-capacitance (ConCap) and constant-current (CC) measurements for EIS and LAPS sensors, respectively, which revealed an enhanced detection signal and sensitivity of ca. 100 mV/decade for the field-effect sensors modified with the PAMAM/SWNT LbL film. It is concluded that controlling film morphology is essential for an enhanced performance of biosensors, not only in terms of sensitivity but also stability and response time.
Die Spritzbetonbauweise hat sich im Tunnelbau seit Jahren bewährt. Spritzbeton wird als unbewehrter Beton, in Verbindung mit Bewehrung sowie als Stahlfaserspritzbeton eingesetzt. Bei den meisten Tunnelschalen aus Stahlfaserspritzbeton werden Stahlfasern derzeit nur als konstruktive Bewehrung verwendet. Die Faserzugabe führt zu einer nennenswerten Erhöhung der Bruchenergie und somit zu einer höheren Zähigkeit des sonst spröden Materials und damit zu einer höheren Sicherheit.
Stahlfaserspritzbeton kann auch anstelle von Spritzbeton mit statisch erforderlicher Mattenbewehrung eingesetzt werden. Die Bemessung erfolgt dann unter Berücksichtigung der Nachrisszugfestigkeit. Diese kann wie bei Stahlfaserbeton an Biegebalken ermittelt werden, die aus größeren Proben herausgesägt werden. Anhand von verschiedenen Beispielen werden die unterschiedlichen Anwendungen aufgezeigt und die dafür erforderlichen Prüfungen vorgestellt.
Ullrich, Sebastian ; Grottke, Oliver ; Rossaint, Rolf ; Staat, Manfred ; Deserno, Thomas M. ; Kuhlen, Torsten
C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli
(2010)
Ribitsch, Doris ; Karl, Wolfgang ; Birner-Gruenberger, R. ; Gruber, K. ; Eiteljoerg, I. ; Remler, Peter ; Wieland, S. ; Siegert, Petra ; Maurer, Karl-Heinz ; Schwab, H.
Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477.
This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7–11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.
Behbahani, Mehdi ; Mai, A. ; Bergmann, B. ; Waluga, C. ; Behr, Marek ; Tran, L. ; Vonderstein, K. ; Mottaghy, K.
Siqueira, Jose R. ; Maki, Rafael M. ; Paulovich, Fernando V. ; Werner, Frederik ; Poghossian, Arshak ; Oliveira, Maria C. F. de ; Zucolotto, Valtencir ; Oliveira, Osvaldo N. Jr. ; Schöning, Michael Josef
The integration of nanostructured films containing biomolecules and silicon-based technologies is a promising direction for reaching miniaturized biosensors that exhibit high sensitivity and selectivity. A challenge, however, is to avoid cross talk among sensing units in an array with multiple sensors located on a small area. In this letter, we describe an array of 16 sensing units of a light-addressable potentiometric sensor (LAPS), which was made with layer-by-layer (LbL) films of a poly(amidomine) dendrimer (PAMAM) and single-walled carbon nanotubes (SWNTs), coated with a layer of the enzyme penicillinase. A visual inspection of the data from constant-current measurements with liquid samples containing distinct concentrations of penicillin, glucose, or a buffer indicated a possible cross talk between units that contained penicillinase and those that did not. With the use of multidimensional data projection techniques, normally employed in information visualization methods, we managed to distinguish the results from the modified LAPS, even in cases where the units were adjacent to each other. Furthermore, the plots generated with the interactive document map (IDMAP) projection technique enabled the distinction of the different concentrations of penicillin, from 5 mmol L−1 down to 0.5 mmol L−1. Data visualization also confirmed the enhanced performance of the sensing units containing carbon nanotubes, consistent with the analysis of results for LAPS sensors. The use of visual analytics, as with projection methods, may be essential to handle a large amount of data generated in multiple sensor arrays to achieve high performance in miniaturized systems
Grottke, O. ; Braunschweig, T. ; Philippen, B. ; Gatzweiler, Karl-Heinz ; Gronloh, N. ; Staat, Manfred ; Rossaint, R. ; Tolba, R.
Background: To elaborate the impact of new haemostatic agents we developed an instrument for the pressure-controlled induction of blunt liver injuries in a porcine animal model. Materials and Methods: A dilutional coagulopathy of 80% of animal blood volume was induced in 9 anaesthetized pigs. Animals were randomly assigned to be injured with a force of 112 Newton (N) (n = 1), 224 ± 19 N (n = 4) or 355 ± 35 N (n = 4). The impact of injury was measured by blood loss, survival time and coagulation parameters. Liver histology was obtained to evaluate the degree of liver injury. Results: The profound haemodilution resulted in a significant alteration of all coagulation parameters. After inflicting the injury with 355 ± 35 N, both the survival time (30 ± 9 min; p = 0.006) and blood loss (68 ± 16 ml min–1, p = 0.002) were significantly different as compared to injuries with 224 ± 19 N (survival time: 76 ± 20 min, blood loss: 23 ± 4 ml min–1). In contrast, an injury with 112 N led to an insignificant blood loss of only 239 ml. Conclusion: We developed a pressure-controlled clamp that allows for the induction of blunt liver traumas with highly reproducible injuries with a positive correlation with blood loss and survival.
Numerical avalanche dynamics models have become an essential part of snow engineering. Coupled with field observations and historical records, they are especially helpful in understanding avalanche flow in complex terrain. However, their application poses several new challenges to avalanche engineers. A detailed understanding of the avalanche phenomena is required to construct hazard scenarios which involve the careful specification of initial conditions (release zone location and dimensions) and definition of appropriate friction parameters. The interpretation of simulation results requires an understanding of the numerical solution schemes and easy to use visualization tools. We discuss these problems by presenting the computer model RAMMS, which was specially designed by the SLF as a practical tool for avalanche engineers. RAMMS solves the depth-averaged equations governing avalanche flow with accurate second-order numerical solution schemes. The model allows the specification of multiple release zones in three-dimensional terrain. Snow cover entrainment is considered. Furthermore, two different flow rheologies can be applied: the standard Voellmy–Salm (VS) approach or a random kinetic energy (RKE) model, which accounts for the random motion and inelastic interaction between snow granules. We present the governing differential equations, highlight some of the input and output features of RAMMS and then apply the models with entrainment to simulate two well-documented avalanche events recorded at the Vallée de la Sionne test site.
Following the success of PET/CT during the last decade and the recent increasing proliferation of SPECT/CT, another hybrid imaging instrument has been gaining more and more interest: MR-PET. First combined, simultaneous PET and MR studies carried out in small animals demonstrated the feasibility of the new approach. Concurrently, some prototypes of an MR-PET scanner for simultaneous human brain studies have been built, their performance is being tested and preliminary applications have already been shown. Through this pioneering work, it has become clear that advances in the detector design are necessary for further optimization.
Recently, the different issues related to the present state and future prospects of MR-PET were presented and discussed during an international 2-day workshop at the Forschungszentrum Jülich, Germany, held after, and in conjunction with, the 2008 IEEE Nuclear Science Symposium and Medical Imaging Conference in Dresden, Germany on October 27–28, 2008. The topics ranged from small animal MR-PET imaging to human MR-BrainPET imaging, new detector developments, challenges/opportunities for ultra-high field MR-PET imaging and considerations of possible future research and clinical applications. This report presents a critical summary of the contributions made to the workshop.
Die SPAC-SE ist börsenfähig und damit eine für eine SPAC grundsätzlich geeignete Rechtsform. Die Tatsache, dass es sich hierbei (zunächst) um eine leere, arbeitnehmerlose Hülle handelt, ändert hieran nichts. Die Gründung einer solchen Vorrats-SE ist trotz fehlender Arbeitnehmerbeteiligung unter teleologischer Reduktion von Art. 12 II SE-VO zulässig. Im Gegenzug muss die Arbeitnehmerbeteiligung gemaß § 18 III SEBG analog nachgeholt werden, wenn das später erworbene Zielunternehmen auf die SPAC-SE verschmolzen werden soll. Aus gesellschaftsrechtlicher Sicht ist zu beachten, dass die SPAC-SE mit Sitz in Deutschland, welche auch den deutschen, aktienrechtlichen Bestimmungen unterliegt, nur bedingt für eine SPAC geeignet erscheint. Das deutsche Aktienrecht enthält strenge Regelungen, die der für eine SPAC-SE erforderlichen Flexibilität entgegenstehen können. Dies gilt insbesondere für das Erfordernis der Zustimmung der Hauptversammlung zur Akquisition des Zielunternehmens, die Rückzahlung des Treuhandvermögens an Aktionäre, die der Akquisition nicht zugestimmt haben und die Liquidation der SPAC-SE im Falle des Scheiterns des Erwerbs des Zielobjektes.
Increasing Scots pine (Pinus sylvestris L.) mortality has been recently observed in the dry inner valleys of the European Alps. Besides drought, infection with pine mistletoe (Viscum album ssp. austriacum) seems to play an important role in the mortality dynamics of Scots pines, but how mistletoes promote pine decline remains unclear. To verify whether pine mistletoe infection weakens the host via crown degradation, as observed for dwarf mistletoes, we studied the negative effects of pine mistletoe infestation on the photosynthetic tissues and branch growth of pairs of infested and non-infested branches. Pine mistletoe infection leads to crown degradation in its host by reducing the length, the radial increment, the ramification, the needle length and the number of needle years of the infested branches. This massive loss in photosynthetic tissue results in a reduction in primary production and a subsequent decrease in carbohydrate availability. The significant reduction in needle length due to mistletoe infection is an indication for a lower water and nutrient availability in infested branches. Thus, mistletoe infection might lead to a decrease in the availability of water and carbohydrates, the two most important growth factors, which are already shortened due to the chronic drought situation in the area. Therefore, pine mistletoe increases the risk of drought-induced mortality of its host when growing in a xeric environment.
Realization of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry
(2010)
Kirchner, Patrick ; Oberländer, Jan ; Friedrich, Peter ; Rysstad, Gunnar ; Berger, Jörg ; Keusgen, Michael ; Schöning, Michael Josef
A calorimetric gas sensor is presented for the monitoring of gas-phase H2O2 at elevated temperature during sterilization processes in aseptic food industry. The sensor consists of two temperature-sensitive thin-film resistances built up on a polyimide foil with a thickness of 25 μm, which are passivated with a layer of SU-8 photo resist and catalytically activated with manganese(IV) oxide. Instead of an active heating structure, the calorimetric sensor utilizes the elevated temperature of an evaporated H2O2 aerosol. In an experimental set-up, the sensor has shown a sensitivity of 4.78 °C/(%v/v) in a H2O2 concentration range of 0 to 10% v/v at an evaporation temperature of 240 ∘C. Furthermore, the sensor possesses the same, unchanged sensor signal even at varied evaporation temperatures of the gas stream. The sensor characterization demonstrates the suitability of the calorimetric gas sensor for monitoring the efficiency of sterilization processes.
Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach of read out in a light-addressable potentiometric sensor (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device which allows fast and flexible generation of light patterns. With the help of these light patterns the sensor surface of the LAPS device can be read out sequentially in a raster like scheme (scanning LAPS). The DLP approach has several advantages compared to conventional scanning LAPS set-ups, e.g., the spot size, the shape and the intensity of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of measurement.