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Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off.
Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.
A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.
The anticancer activity of titanium complexes has been known since the groundbreaking studies of Köpf and Köpf-Maier on titanocen dichloride. Unfortunately, possibly due to their fast hydrolysis, derivatives of titanocen dichloride failed in clinical studies. Recently, the new family of titanium salan complexes containing tetradentate ONNO ligands with anti-cancer properties has been discovered. These salan complexes are much more stabile in aqueous media. In this study we describe the biological activity of two titanium salan complexes in a mouse model of cervical cancer. High efficiency of this promising complex family was demonstrated for the first time in vivo. From these data we conclude that titanium salan complexes display very strong antitumor properties exhibiting only minor side effects. Our results may influence the chemotherapy with metallo therapeutics in the future.
Bioconjugates containing the GnRH-III hormone decapeptide as a targeting moiety are able to deliver chemotherapeutic agents specifically to cancer cells expressing GnRH receptors, thereby increasing their local efficacy while limiting the peripheral toxicity. However, the number of GnRH receptors on cancer cells is limited and they desensitize under continuous hormone treatment. A possible approach to increase the receptor mediated tumor targeting and consequently the cytostatic effect of the bioconjugates would be the attachment of more than one chemotherapeutic agent to one GnRH-III molecule. Here we report on the design, synthesis and biochemical characterization of multifunctional bioconjugates containing GnRH-III as a targeting moiety and daunorubicin as a chemotherapeutic agent. Two different drug design approaches were pursued. The first one was based on the bifunctional [4Lys]-GnRH-III (Glp-His-Trp-Lys-His-Asp-Trp-Lys-Pro-Gly-NH2) containing two lysine residues in positions 4 and 8, whose ϵ-amino groups were used for the coupling of daunorubicin. In the second drug design, the native GnRH-III (Glp-His-Trp-Ser-His-Asp-Trp-Lys-Pro-Gly-NH2) was used as a scaffold; an additional lysine residue was coupled to the ϵ-amino group of 8Lys in order to generate two free amino groups available for conjugation of daunorubicin. The in vitro stability/degradation of all synthesized compounds was investigated in human serum, as well as in the presence of rat liver lysosomal homogenate. Their cellular uptake was determined on human breast cancer cells and the cytostatic effect was evaluated on human breast, colon and prostate cancer cell lines. Compared with a monofunctional compound, both drug design approaches resulted in multifunctional bioconjugates with increased cytostatic effect.
Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
(2011)
Suburethral slings as well as different meshes are widely used treating stress urinary incontinence and prolaps in women. With the development of MiniSlings and special meshes using less alloplastic material anchorage systems become more important to keep devices in place and to put some tension especially on the MiniSlings. To date, there are many different systems of MiniSlings of different companies on the market which differ in the structure of the used meshes and anchors. A new objective measurement method to compare different properties of MiniSling systems (mesh and anchor) is presented in this article. Ballistic gelatine acts as soft tissue surrogate. Significant differences in parameters like pull-out strength of anchors or shrinkage of meshes under loading conditions have been determined. The form and size of the anchors as well as the structural stability of the meshes are decisive for a proper integration. The tested anchorings sytems showed markedly different mechanical function at their respective load bearing capacity. As the stable fixation of the device in tissue is a prerequisite for a permanet reinforcement, the proposed test system permits further optimisation of anchor and mesh devices to improve the success of the surgical treatment
Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions.
Testing of a 10 kW diffusive micro-mix combustor for hydrogen-fuelled micro-scale gas turbines
(2007)
With the final objective of optimizing the "Micromix" hydrogen combustion principle, a round jet in a laminar cross-flow prior to its combustion is investigated experimentally using Stereoscopic Particle Image Velocimetry. Measurements are performed at a jet to cross-stream momentum ratio of 1 and a Reynolds number, based on the jet diameter and jet velocity, of 1600. The suitability to combine side, top and end views is analyzed statistically. The statistical theory of testing hypotheses, pertaining to the joint distribution of the averaged velocity along intersecting observation planes, is employed. Overall, the averaged velocity fields of the varying observation planes feature homogeneity at a 0.05 significance level. Minor discrepancies are related to the given experimental conditions. By use of image maps, averaged and instantaneous velocity fields, an attempt is made to elucidate the flow physics and a kinematically consistent vortex model is proposed. In the time-averaged flow field, the principal vortical systems were identified and the associated mixing visualized. The jet trajectory and physical dimensions scale with the momentum ratio times the jet diameter. The jet/cross-flow mixture converging upon the span-wise centre-line, the lifting action of the Counter Rotating Vortex Pair and the reversed flow region contribute to the high entrainment and mixedness. It is shown that the jet width is larger on the downstream side as compared to the upstream side of the centre-streamline. The deepest penetration of the particles on the outer boundary occurs in the centre-plane. Meanwhile, with increasing off-centre position, the boundaries all lay further from the centre-line position than does the boundary in the centre-plane, corresponding to a kidney-like shape of the flow cross-section. The generation of the Counter Rotating Vortex Pair and the instability mechanism is documented by instantaneous image maps and vector fields. The necessary circulation for the Counter Rotating Vortex Pair originates from a combined effect of steady in-hole, hanging and wake vortices. The strong cross-flow and jet interaction induces a three-dimensional waving, the stream-wise Counter Rotating Vortex Pair pair, leading to the formation of Ring Like Vortices. A secondary Counter Rotating Vortex Pair forms on top of the primary Counter Rotating Vortex Pair, resulting in mixing by "puffs". Overall, Stereoscopic Particle Image Velocimetry proofed capable of elucidating the Jet in Cross-Flow complex flow field. The gained insight in the mixing process will definitely contribute to the "Micromix" hydrogen combustion optimization.
For more than a decade up to now there is an ongoing interest in small gas turbines downsized to micro-scale. With their high energy density they offer a great potential as a substitute for today’s unwieldy accumulators, found in a variety of applications like laptops, small tools etc. But micro-scale gas turbines could not only be used for generating electricity, they could also produce thrust for powering small unmanned aerial vehicles (UAVs) or similar devices. Beneath all the great design challenges with the rotating parts of the turbomachinery at this small scale, another crucial item is in fact the combustion chamber needed for a safe and reliable operation. With the so called regular micromix burning principle for hydrogen successfully downscaled in an initial combustion chamber prototype of 10 kW energy output, this paper describes a new design attempt aimed at the integration possibilities in a μ-scale gas turbine. For manufacturing the combustion chamber completely out of stainless steel components, a recuperative wall cooling was introduced to keep the temperatures in an acceptable range. Also a new way of an integrated ignition was developed. The detailed description of the prototype’s design is followed by an in depth report about the test results. The experimental investigations comprise a set of mass flow variations, coupled with a variation of the equivalence ratio for each mass flow at different inlet temperatures and pressures. With the data obtained by an exhaust gas analysis, a full characterisation concerning combustion efficiency and stability of the prototype chamber is possible. Furthermore the data show a full compliance with the expected operating requirements of the designated μ-scale gas turbine.
One-chip integrated dual amperometric/field-effect sensor for the detection of dissolved hydrogen
(2011)
Media Delivery Based on Service Aware Transport Overlay Networks / Kampmann, Markus ; Hartung, Frank
(2007)
Among many approaches to address the high-level decision making problem for autonomous robots and agents, the robot program¬ming and plan language Golog follows a logic-based deliberative approach, and its successors were successfully deployed in a number of robotics applications over the past ten years. Usually, Golog interpreter are implemented in Prolog, which is not available for our target plat¬form, the bi-ped robot platform Nao. In this paper we sketch our first approach towards a prototype implementation of a Golog interpreter in the scripting language Lua. With the example of the elevator domain we discuss how the basic action theory is specified and how we implemented fluent regression in Lua. One possible advantage of the availability of a Non-Prolog implementation of Golog could be that Golog becomes avail¬able on a larger number of platforms, and also becomes more attractive for roboticists outside the Cognitive Robotics community.