Refine
Year of publication
Document Type
- Article (585)
- Patent (117)
- Book (65)
- Conference Proceeding (33)
- Part of a Book (21)
- Report (4)
- Doctoral Thesis (3)
- Preprint (1)
Language
- English (522)
- German (304)
- Multiple languages (2)
- Spanish (1)
Has Fulltext
- no (829) (remove)
Keywords
- Heparin (3)
- Chemometrics (2)
- IR spectroscopy (2)
- NMR spectroscopy (2)
- Principal component analysis (2)
- Standardization (2)
- (R)- or (S)- gamma-valerolactone (1)
- 4-hydroxy valeric acid (1)
- Alginate beads (1)
- Analytics (1)
- Authenticity (1)
- Bioeconomy (1)
- Bioethanol (1)
- Biomass (1)
- Biorefinery (1)
- Biorefinery definitions (1)
- Bladder (1)
- Bragg peak (1)
- CRISPR/Cas9 (1)
- Chimeric liver-humanized mice (1)
- Chiralidon-R (1)
- Chiralidon-S (1)
- Crude heparin (1)
- Cyclotron production (1)
- Decentral (1)
- Dehydrogenase (1)
- Detergent protease (1)
- Deuterated solvents (1)
- Deuterium NMR (1)
- Diaphorase (1)
- Drug distribution (1)
- Drug metabolism (1)
- Enzymatic biosensor (1)
- Extracellular enzymes (1)
- Ga-68 (1)
- Growth modelling (1)
- Hypersecretion (1)
- IR (1)
- Inorganic ions (1)
- Introduction (1)
- Ions (1)
- Knockout mice (1)
- Levulinic acid (1)
- Lignocellulose feedstook (1)
- Linear discriminant analysis (1)
- Manufacturer (1)
- Marker-free mutagenesis (1)
- Mechanical (1)
- Mechanical simulation (1)
- Medical radionuclide production (1)
- Metal contaminants (1)
- Microfluidic solvent extraction (1)
- Minor chemistry (1)
- Molecular modelling (1)
- Molecular weight determination (1)
- NMR (1)
- On-site (1)
- P2G (1)
- PLS-regression (1)
- Physical chemistry (1)
- Physical chemistry basics (1)
- Physical chemistry starters (1)
- Physikalische Chemie (1)
- Pre-treatment (1)
- Process schemes (1)
- Quality control (1)
- Quantum chemistry (1)
- Reconstruction (1)
- Renewable resources (1)
- Simultaneous determination (1)
- Soft independent modeling of class analogy (1)
- Stenotrophomonas maltophilia (1)
- Thermodynamics as minor (1)
- Toxicology (1)
- USP (1)
- Uracil-phosphoribosyltransferase (1)
- Ureter (1)
- actuator-sensor system (1)
- aspergillus (1)
- bacterial cellulose (1)
- bi-enzyme biosensor (1)
- bioavailability (1)
- biodegradable polymers (1)
- biological dosimeter (1)
- biomethane (1)
- borehole disposal (1)
- bubble column (1)
- capacitive field-effect sensor (1)
- coculture (1)
- deficit irrigation (1)
- disposal facility (1)
- drug metabolising enzymes (1)
- drug–drug interactions (1)
- elastomers (1)
- enzyme kinetics (1)
- enzyme-logic gate (1)
- exopolysaccharides (1)
- filamentous fungi (1)
- genome engineering (1)
- geological disposal (1)
- glycine (1)
- human metabolites (1)
- hydrogel (1)
- hydrogels (1)
- light-addressable electrode (1)
- light-addressable potentiometric sensor (1)
- mechanical properties (1)
- methanation (1)
- microfluidics (1)
- micronutrients (1)
- neutrons (1)
- nuclear waste (1)
- onion (1)
- optical fibers (1)
- penicillinase (1)
- plug flow reactor (1)
- polyaspartic acid (1)
- prebiotic (1)
- proton therapy (1)
- protons (1)
- pullulan (1)
- qNMR (1)
- relative dosimetry (1)
- retention time (1)
- rubber (1)
- superabsorbent polymers (1)
- supramolecular structures (1)
- swelling properties (1)
- theory and modeling (1)
- tobacco mosaic virus (TMV) (1)
- transporters (1)
- urease (1)
- water economy (1)
- yield (1)
Institute
- Fachbereich Chemie und Biotechnologie (829) (remove)
Grassilage stellt einen nachwachsenden Rohstoff mit großem Potenzial dar. Neben Cellulose und Hemicellulose enthält sie auch organische Säuren, insbesondere Milchsäure. In einem Bioraffinerie-Projekt wird die Milchsäure aus der Silage isoliert und mit gentechnisch optimierten Stämmen zu L-Lysin weiterverarbeitet. Die Lignocellulose wird hydrolysiert und zu Ethanol fermentiert. Ein besonderes Augenmerk liegt auf der Integration der unterschiedlichen Prozesse sowie der einzelnen Prozessschritte zu einem Gesamtprozess, der sämtliche Inhaltsstoffe der Silage verwertet.
Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.
Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7
(2011)