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Keywords
Jiminez German, Salvador ; Behbahani, Mehdi ; Miettinen, Susanna ; Grijpma, Dirk W. ; Haimi, Suvi P.
Jakob, Felix ; Martinez, Ronny ; Mandawe, John ; Hellmuth, Hendrik ; Siegert, Petra ; Maurer, Karl-Heinz ; Schwaneberg, Ulrich
The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the spatial distribution of specific ions on the sensing surface. The conventional chemical imaging system based on the light-addressable potentiometric sensor (LAPS), however, required a long time to obtain a chemical image, due to the slow mechanical scan of a single light beam. For high-speed imaging, a plurality of light beams modulated at different frequencies can be employed to measure the ion concentrations simultaneously at different locations on the sensor plate by frequency division multiplex (FDM). However, the conventional measurement geometry of back-side illumination limited the bandwidth of the modulation frequency required for FDM measurement, because of the low-pass filtering characteristics of carrier diffusion in the Si substrate. In this study, a high-speed chemical imaging system based on front-side-illuminated LAPS was developed, which achieved high-speed spatiotemporal recording of pH change at a rate of 70 frames per second.
Iken, Heiko ; Ahlborn, Kristina ; Gerlach, Frank ; Vonau, Winfried ; Zander, Wilhelm ; Schubert, Jürgen P. ; Schöning, Michael Josef
Huck, Christina ; Schiffels, Johannes ; Herrera, Cony N. ; Schelden, Maximilian ; Selmer, Thorsten ; Poghossian, Arshak ; Baumann, Marcus ; Wagner, Patrick ; Schöning, Michael Josef
Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
Chemische Sensoren mit Bariumstrontiumtitanat als funktionelle Schicht zur Multiparameterdetektion
(2013)
Huck, Christina ; Poghossian, Arshak ; Bäcker, Matthias ; Zander, W. ; Schubert, J. ; Sukoyan, L. H. ; Begoyan, V. ; Buniatyan, V. V. ; Wagner, Patrick ; Schöning, Michael Josef
Das kompakte Nachschlagewerk für den konstruktiven Ingenieurbau wurde für den täglichen Gebrauch auf der Baustelle konzipiert und stellt alle für die rechnerische Nachweisführung und Konstruktion von Bauteilen wichtigsten Angaben in Form einer Formelsammlung mit Nachweishilfen zur Verfügung. Der Inhalt wurde auf Basis der neuen Eurocodes komplett aktualisiert und erweitert. Die 4. Auflage enthält in bewährter Manier eine umfangreiche Formelsammlung, Querschnittswerte und Bemessungshilfen zu den Bereichen Lastannahmen, Holzbau, Mauerwerksbau, Stahlbau, Stahlbetonbau, Geotechnik sowie statische Hinweise. Farbiges Papier erleichtert den zielgerichteten Zugriff auf die einzelnen Fachgebiete.