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Year of publication
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- INB - Institut für Nano- und Biotechnologien (524) (remove)
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Keywords
The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) inthe sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface.A reduced impedance inside the defect, which was artificially formed ina cell layer, was successfully visualized in a photocurrent image.
The chemical imaging sensor, which is based on the principle of the light-addressable potentiometric sensor (LAPS), is a powerful tool to visualize the spatial distribution of chemical species on the sensor surface. The spatial resolution of this sensor depends on the diffusion of photocarriers excited by a modulated light. In this study, a novel hybrid fiber-optic illumination was developed to enhance the spatial resolution. It consists of a modulated light probe to generate a photocurrent signal and a ring of constant light, which suppresses the lateral diffusion of minority carriers excited by the modulated light. It is demonstrated that the spatial resolution was improved from 92 μm to 68 μm.
Miyamoto, K. ; Kuwabara, Yohei ; Kanoh, Shin'ichiro ; Yoshinobu, Tatsuo ; Wagner, Torsten ; Schöning, Michael Josef
The chemical imaging sensor is a chemical sensor which is capable of visualizing the spatial distribution of chemical species in sample solution. In this study, a novel measurement system based on the chemical imaging sensor was developed to observe the inside of a Y-shaped microfluidic channel while injecting two sample solutions from two branches. From the collected chemical images, it was clearly observed that the injected solutions formed laminar flows in the microfluidic channel. In addition, ion diffusion across the laminar flows was observed. This label-free method can acquire quantitative data of ion distribution and diffusion in microfluidic devices, which can be used to determine the diffusion coefficients, and therefore, the molecular weights of chemical species in the sample solution.
Miyamato, Ko-ichiro ; Sakakita, Sakura ; Wagner, Torsten ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
The chemical imaging sensor was applied to in-situ pH imaging of the solution in the vicinity of a corroding surface of stainless steel under potentiostatic polarization. A test piece of polished stainless steel was placed on the sensing surface leaving a narrow gap filled with artificial seawater and the stainless steel was corroded under polarization. The pH images obtained during polarization showed correspondence between the region of lower pH and the site of corrosion. It was also found that the pH value in the gap became as low as 2 by polarization, which triggered corrosion.
Mikulics, M. ; Marso, M. ; Cámara Mayorga, I. ; Gusten, R. ; Stancek, S. ; Michael, E. A. ; Schieder, R. ; Wolter, M. ; Buca, D. ; Förster, Arnold ; Kordos, P. ; Lüth, H.
Mikulics, M. ; Camara, I. ; Hardt, A. van der ; Fox, A. ; Förster, Arnold ; Gusten, R. ; Lüth, H. ; Kordos, P.
Mikulics, M. ; Adam, R. ; Kordos, P. ; Förster, Arnold ; Lüth, H. ; Wu, S. ; Zheng, X. ; Sobolewski, R.
Transport through Redox-Active Ru-Terpyridine Complexes Integrated in Single Nanoparticle Devices
(2020)
Transition metal complexes are electrofunctional molecules due to their high conductivity and their intrinsic switching ability involving a metal-to-ligand charge transfer. Here, a method is presented to contact reliably a few to single redox-active Ru-terpyridine complexes in a CMOS compatible nanodevice and preserve their electrical functionality. Using hybrid materials from 14 nm gold nanoparticles (AuNP) and bis-{4′-[4-(mercaptophenyl)-2,2′:6′,2″-terpyridine]}-ruthenium(II) complexes a device size of 30² nm² inclusive nanoelectrodes is achieved. Moreover, this method bears the opportunity for further downscaling. The Ru-complex AuNP devices show symmetric and asymmetric current versus voltage curves with a hysteretic characteristic in two well separated conductance ranges. By theoretical approximations based on the single-channel Landauer model, the charge transport through the formed double-barrier tunnel junction is thoroughly analyzed and its sensibility to the molecule/metal contact is revealed. It can be verified that tunneling transport through the HOMO is the main transport mechanism while decoherent hopping transport is present to a minor extent.
Martinez, Ronny ; Jakob, Felix ; Tu, Ran ; Siegert, Petra ; Maurer, Karl-Heinz ; Schwaneberg, Ulrich
Malzahn, Kerstin ; Windmiller, Joshua Ray ; Valdés-Ramírez, Gabriela ; Wang, Joseph ; Schöning, Michael Josef
Leinhos, Marcel ; Schusser, Sebastian ; Bäcker, Matthias ; Poghossian, Arshak ; Schöning, Michael Josef
It is well known that the degradation environment can strongly influence the biodegradability and kinetics of biodegradation processes of polymers. Therefore, besides the monitoring of the degradation process, it is also necessary to control the medium in which the degradation takes place. In this work, a micromachined multi-parameter sensor chip for the control of the polymer-degradation medium has been developed. The chip combines a capacitive field-effect pH sensor, a four-electrode electrolyte-conductivity sensor and a thin-film Pt-temperature sensor. The results of characterization of individual sensors are presented. In addition, the multi-parameter sensor chip together with an impedimetric polymer-degradation sensor was simultaneously characterized in degradation solutions with different pH and electrolyte conductivity. The obtained results demonstrate the feasibility of the multi-parameter sensor chip for the control of the polymer-degradation medium.
Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
(2014)
Küppers, Tobias ; Steffen, Victoria ; Hellmuth, Hendrik ; O'Connell, Timothy ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Wiechert, Wolfgang