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Keywords
Welden, Melanie ; Poghossian, Arshak ; Vahidpour, Farnoosh ; Wendlandt, Tim ; Keusgen, Michael ; Wege, Christina ; Schöning, Michael Josef
Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.
Detection of triacetone triperoxide using temperature cycled metal-oxide semiconductor gas sensors
(2015)
Wagner, Torsten ; Werner, Frederik ; Miyamoto, Ko-Ichiro ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach to read out light-addressable potentiometric sensors (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device, which allows fast and flexible generation of light patterns. With the help of these light patterns, the sensor surface of the LAPS device can be addressed. The DLP approach has several advantages compared to conventional LAPS set-ups, e.g., the spot size and the shape of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of the measurement. In addition, the modulation frequency and intensity of the light beam are important parameters of the LAPS set-up. Within this work, the authors will discuss two different ways of light modulation by the DLP set-up, investigate the influence of different modulation frequencies and different light intensities as well as demonstrate the scanning capabilities of the new set-up by pH mapping on the sensor surface.
Wagner, Torsten ; Werner, Frederik ; Miyamoto, Ko-Ichiro ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
Wagner, Torsten ; Werner, Frederik ; Miyamoto, Ko-ichiro ; Ackermann, Hans-Josef ; Yoshinobu, Tatsuo ; Schöning, Michael Josef
Wagner, Torsten ; Vornholt, Wolfgang ; Werner, Frederik ; Yoshinobu, Tatsuo ; Miyamoto, Ko-Ichiro ; Keusgen, Michael ; Schöning, Michael Josef
The light-addressable potentiometric sensor (LAPS) has the unique feature to address different regions of a sensor surface without the need of complex structures. Measurements at different locations on the sensor surface can be performed in a common analyte solution, which distinctly simplifies the fluidic set-up. However, the measurement in a single analyte chamber prevents the application of different drugs or different concentrations of a drug to each measurement spot at the same time as in the case of multi-reservoir-based set-ups. In this work, the authors designed a LAPS-based set-up for cell culture screening that utilises magnetic beads loaded with the endotoxin (lipopolysaccharides, LPS), to generate a spatially distributed gradient of analyte concentration. Different external magnetic fields can be adjusted to move the magnetic beads loaded with a specific drug within the measurement cell. By recording the metabolic activities of a cell layer cultured on top of the LAPS surface, this work shows the possibility to apply different concentrations of a sample along the LAPS measurement spots within a common analyte solution.
Wagner, Torsten ; Shigiahara, N. ; Miyamoto, K. ; Suzurikawa, J. ; Finger, F. ; Schöning, Michael Josef ; Yoshinobu, T.
This work describes the novel combination of the light-addressable electrode (LAE) and the light-addressable potentiometric sensor (LAPS) into a microsystem set-up. Both the LAE as well as the LAPS shares the principle of addressing the active spot by means of a light beam. This enables both systems to manipulate resp. to detect an analyte with a high spatial resolution. Hence, combining both principles into a single set-up enables the active stimulation e.g., by means of electrolysis and a simultaneous observation e.g., the response of an entrapped biological cell by detection of extracellular pH changes. The work will describe the principles of both technologies and the necessary steps to integrate them into a single set-up. Furthermore, examples of application and operation of such systems will be presented.
Wagner, Torsten ; Rao, C. ; Kloock, Joachim P. ; Yoshinobu, T. ; Otto, R. ; Keusgen, M. ; Schöning, Michael Josef
Wagner, Torsten ; Molina, Roberto ; Yoshinobu, Tatsuo ; Kloock, Joachim P. ; Biselli, Manfred ; Canzoneri, Michele ; Schnitzler, Thomas ; Schöning, Michael Josef
Wagner, Torsten ; Molina, R. ; Biselli, Manfred ; Canzoneri, Michele ; Schnitzler, Thomas ; Yoshinobu, Tatsuo ; Schöning, Michael Josef
Wagner, Torsten ; Miyamoto, Ko-ichiro ; Shigihara, Noriko ; Schöning, Michael Josef ; Yoshinobu, Tatsuo