Article
Refine
Author
- Selmer, Thorsten (49)
- Bongaerts, Johannes (46)
- Biselli, Manfred (45)
- Scheer, Nico (34)
- Schöning, Michael Josef (32)
- Tippkötter, Nils (30)
- Mang, Thomas (27)
- Scherer, Ulrich W. (27)
- Seibler, Jost (26)
- Siegert, Petra (26)
- Baumann, Marcus (22)
- Paulßen, Elisabeth (22)
- Ulber, Roland (21)
- Maurer, Karl-Heinz (17)
- Wolf, C. Roland (17)
- Öhlschläger, Peter (17)
- Prielmeier, Franz (16)
- Elbers, Gereon (15)
- Monakhova, Yulia (15)
- Wagner, Torsten (15)
- Rode, Anja (13)
- Berndt, Heinz (12)
- Keusgen, Michael (11)
- Kotter, Michael (11)
- Hojdis, Nils (10)
- Pohl, Martina (10)
- Evers, Stefan (9)
- Hoehr, Cornelia (9)
- Poghossian, Arshak (9)
- Abram, Ulrich (8)
- Lang, E. W. (8)
- Lüdemann, H.-D. (8)
- Wandrey, Christian (8)
- Kapelyukh, Yury (7)
- Kricheldorf, Hans R. (7)
- Wandrey, C. (7)
- Wubbolts, Marcel (7)
- Albrecht, Dirk (6)
- Bode, Jürgen (6)
- Brüning, Jens C. (6)
- Campos-Ortega, José A. (6)
- Hecker, Michael (6)
- Henderson, Colin J. (6)
- Jeromin, Günter Erich (6)
- Kratz, J. V. (6)
- Molinnus, Denise (6)
- Pilas, Johanna (6)
- Schiffels, Johannes (6)
- Schnitzler, Thomas (6)
- Van der Pol, Jens J. (6)
- Voigt, Birgit (6)
- Born, C. (5)
- Bott, Michael (5)
- Brüchle, W. (5)
- Buckel, Wolfgang (5)
- Bäcker, Matthias (5)
- Gäggeler, H. W. (5)
- Karimi-Varzaneh, Hossein Ali (5)
- Krämer, Marco (5)
- Lauth, Jakob (5)
- Merschenz-Quack, Angelika (5)
- Muschallik, Lukas (5)
- Müller, Michael (5)
- Osen, Wolfram (5)
- Ross, Jillian (5)
- Schulze, Margit (5)
- Schwenk, Frieder (5)
- Schädel, M. (5)
- Thömmes, J. (5)
- Unden, Gottfried (5)
- Yoshinobu, Tatsuo (5)
- Al-Kaidy, Huschyar (4)
- Bovenberg, Roel (4)
- Daniel, Rolf (4)
- Demir, Ayhan S. (4)
- Diehl, Bernd W. K. (4)
- Diehl, Bernd W.K. (4)
- Engel, Mareike (4)
- Falkenberg, Fabian (4)
- Feuerriegel, Uwe (4)
- Hager, Jonathan (4)
- Hentschke, Reinhard (4)
- Holtmann, Dirk (4)
- Höcker, Hartwig (4)
- Kolz, J. (4)
- Krafft, Simone (4)
- Lehmann, G. (4)
- Liesegang, Heiko (4)
- Muffler, Kai (4)
- Müller, Ulrike (4)
- Raeven, Leon (4)
- Raue, Markus (4)
- Riekert, L. (4)
- Riekert, Lothar (4)
- Schmidt, Bernhard (4)
- Schweder, Thomas (4)
- Schübeler, Dirk (4)
- Sohling, Ulrich (4)
- Speedy, R. J. (4)
- Srivastava, Alok (4)
- Stanley, Lesley A. (4)
- Suck, Kirstin (4)
- Takors, Ralf (4)
- Temiz Artmann, Aysegül (4)
- Zimmermann, H. P. (4)
- Adels, Klaudia (3)
- Aletsee, Ludwig (3)
- Alvarez, Gerardo (3)
- Andrei, Paula I. (3)
- Artmann, Gerhard (3)
- Becher, Dörte (3)
- Breuer, Lars (3)
- Burger, René (3)
- Christmann, K. (3)
- Do, Xuan Tung (3)
- Dünnwald, Thomas (3)
- Elcombe, Clifford R. (3)
- Everaers, Ralf (3)
- Figura, Kurt von (3)
- Fleck, Franz (3)
- Frahm, Jens (3)
- Gober, M. K. (3)
- Greguric, Ivan (3)
- Grötzinger, Joachim (3)
- Haeger, Gerrit (3)
- Hellmuth, Hendrik (3)
- Hilbert, U. (3)
- Hoffstadt, Kevin (3)
- Iding, Hans (3)
- Jablonski, Melanie (3)
- Jost, D. T. (3)
- Jürgen, Britta (3)
- Keusgen, M. (3)
- Kuperjans, Isabel (3)
- Küter-Luks, Birgit (3)
- Lalk, Michael (3)
- Lassonczyk, Beate (3)
- Mclaughlin, Lesley A. (3)
- Meinhardt, Friedhelm (3)
- Mootz, D. (3)
- Nagatomo, Yasushi (3)
- Noll, T. (3)
- Noll, Thomas (3)
- O'Connell, Timothy (3)
- Pierik, Antonio J. (3)
- Pinkenburg, Olaf (3)
- Radkowitsch, H. (3)
- Remme, S. (3)
- Ruf, Friedrich (3)
- Rumpf, Jessica (3)
- Schaffer, Paul (3)
- Schmidt, S. (3)
- Schroeter, Rebecca (3)
- Six, S. (3)
- Sprenger, G. (3)
- Svaneborg, Carsten (3)
- Türler, A. (3)
- Unden, G. (3)
- Wagner, Patrick (3)
- Wege, Christina (3)
- Zang, Werner (3)
- Aggarwal, Pranav (2)
- Bauer, Bernhard L. (2)
- Baumann, Martin (2)
- Becker, S. (2)
- Benard, Francois (2)
- Bertalanffy, Helmut (2)
- Beuger, Vincent (2)
- Bial, J. (2)
- Biel, Markus (2)
- Birner-Gruenberger, R. (2)
- Blaser, Martin (2)
- Braband, Henrik (2)
- Braeuning, Albert (2)
- Buckley, Ken (2)
- Buechel, Sandra (2)
- Burmistrova, Natalia A. (2)
- Bélanger-Champagne, Camille (2)
- Büchs, Jochen (2)
- Canzoneri, Michele (2)
- Capitain, Charlotte (2)
- Celler, Anna (2)
- Cheenakula, Dheeraja (2)
- Chen, Liangfu (2)
- Chibout, Salah-Dine (2)
- Chu, Xiaoyan (2)
- Couttet, Philippe (2)
- Dallas, Shannon (2)
- Delaittre, Guillaume (2)
- Deppe, Veronika Maria (2)
- Dierks, Thomas (2)
- Druckenmüller, Katharina (2)
- Duwe, Anna-Maria (2)
- Dürst, Matthias (2)
- Eckert, Alexander (2)
- Eisenkrätzer, Detlef (2)
- Eiteljoerg, I. (2)
- Elskens, M. (2)
- Faust, Nicole (2)
- Ferguson, Simon (2)
- Fiering, Steven (2)
- Gerigk, M. (2)
- Gradinger, R. (2)
- Grenet, Olivier (2)
- Groebel, Simone (2)
- Groth, Ulrich (2)
- Gruber, K. (2)
- Hampel, Brigitte (2)
- Handtke, Stefan (2)
- Hanemaayer, Victoire (2)
- Hans, Stefan (2)
- Harms, Hauke (2)
- Haulena, Friedhelm (2)
- Henderson, Colin James (2)
- Herfurth, C. (2)
- Hetzel, Marc (2)
- Hirschwald, W. (2)
- Hoelderich, W. (2)
- Horsburgh, Brian C. (2)
- Hou, Xinchi (2)
- Huhn, Thomas (2)
- Hör, G. (2)
- Iber, Michaela (2)
- Ingendoh, Arnd (2)
- Jaeger, Karl-Erich (2)
- Jahnke, J. (2)
- Jakob, Felix (2)
- Jelinek, N. (2)
- Jennemann, Richard (2)
- Joksch, B. (2)
- Jonkers, Jos (2)
- Jonté, J. Michael (2)
- Jossek, Ralf (2)
- Kalbe, Jochen (2)
- Karl, W. (2)
- Kauselmann, Gunther (2)
- Kern, Heidrun (2)
- Kipp, Carina Ronja (2)
- Klose, W. (2)
- Koch, Linda (2)
- Kragl, U. (2)
- Krauss, Manuel (2)
- Kremer, K. (2)
- Krumbe, Christoph (2)
- Krüger, Götz (2)
- Kunta, Jeevan (2)
- Kuropka, Rolf (2)
- Könner, A. Christine (2)
- Kühn, Ralf (2)
- Laplanche, Loic (2)
- Le, Van So (2)
- Lempiäinen, Harri (2)
- Lengkeek, Nigel A. (2)
- Leurs, Ulrike (2)
- Lin, De (2)
- Lindner, Simon (2)
- Lintz, Hans-Günther (2)
- Luisier, Raphaëlle (2)
- Lüllau, E. (2)
- MacLeod, Alastair Kenneth (2)
- Manea, Marilena (2)
- Martinez, Ronny (2)
- Martins, J. (2)
- Meehan, Richard R. (2)
- Mesch, K. (2)
- Methling, Karen (2)
- Meyer-Stork, L. Sebastian (2)
- Mezler, Mario (2)
- Moggs, Jonathan G. (2)
- Mostacci, Domiziano (2)
- Moulin, Pierre (2)
- Mues genannt Koers, Lucas (2)
- Müller, Arne (2)
- Niedermeier, Jana (2)
- Niehaves, Sandra (2)
- Nikolausz, Marcell (2)
- Nokihara, Kiyoshi (2)
- Orban, Erika (2)
- Pang, Jodie (2)
- Pellegrini, Paul A. (2)
- Penner, Crystal (2)
- Plum, Leona (2)
- Quetting, Michael (2)
- Rehahn, Matthias (2)
- Remler, P. (2)
- Ribitsch, D. (2)
- Riedl, Iris (2)
- Riley, R. J. (2)
- Roth, Jasmine (2)
- Runstadler, P. W. (2)
- Röhlen, Desiree (2)
- Schelden, Maximilian (2)
- Schirawski, J. (2)
- Schmitz, Dominik (2)
- Schneider, Felix (2)
- Schreiter, K. (2)
- Schröder, B. (2)
- Schwab, H. (2)
- Schwaneberg, Ulrich (2)
- Schwarz, Michael (2)
- Seifarth, Volker (2)
- Semeneh, M. (2)
- Sieker, Tim (2)
- Singh, Virendra (2)
- Soboleva, Polina M. (2)
- Solomun, T. (2)
- Sprenger, Georg A. (2)
- Stahlberg, R. (2)
- Stiefelmaier, Judith (2)
- Takenaga, Shoko (2)
- Thiel, Alexander (2)
- Thoelen, R. (2)
- Thomson, John P. (2)
- Trinczek, Michael (2)
- Tüg, Helmut (2)
- Usherovich, Samuel (2)
- Volland, Sonja (2)
- Wagenblast, G. (2)
- Wagner, P. (2)
- Walther, Andreas (2)
- Wehr, R. (2)
- Weiner, Ron (2)
- Welden, Melanie (2)
- Werner, Frederik (2)
- Wick, Markus (2)
- Wiegand, Sandra (2)
- Wieland, S. (2)
- Wientjens, Ellen (2)
- Wiesen, Sebastian (2)
- Wilson, E. M. (2)
- Wilson, Ian D. (2)
- Wolf, Roland C. (2)
- Wollny, Steffen (2)
- Wunderlich, F. Thomas (2)
- Yabut, Jocelyn (2)
- Zeisler, Stefan (2)
- Zevnik, Branko (2)
- Abbasi, Mozhdeh (1)
- Abetz, C. (1)
- Abetz, V. (1)
- Abouabdillah, Aziz (1)
- Aboudrare, Abdellah (1)
- Aboulnaga, El-Hussiny A. A. (1)
- Aboulnaga, Elhussiny A. (1)
- Abulnaga, El-Hussiny (1)
- Achebach, Stephanie (1)
- Adam, Michael J. (1)
- Adler, Thure (1)
- Aggarwal, Prerna (1)
- Akpinar, Hale (1)
- Al-Khasawneh, Kafa (1)
- Al-Momani, Lóay (1)
- Alaily, F. (1)
- Alami, Raouf (1)
- Alber, Jens (1)
- Alberto, Roger (1)
- Albertz, Michael (1)
- Albracht, Kirsten (1)
- Alenina, Natalia (1)
- Aliotta, M. (1)
- Alkemade, J. (1)
- Alloza, Leonor (1)
- Almajhdi, Fahad N. (1)
- Ammann, Markus (1)
- Anastassiadis, Konstantinos (1)
- Andonian, Clara (1)
- Andreas, Susanne (1)
- Andrei-Selmer, Luminita-Cornelia (1)
- Ansorge, Marion (1)
- Ansorge-Schumacher, Marion (1)
- Arciszewski, Pawel (1)
- Armbruster, P. (1)
- Arndt, Patrick (1)
- Ashcroft, Frances M. (1)
- Atmane, Ilyas (1)
- Au, M. (1)
- Baarends, Willy M. (1)
- Bacher, Michael (1)
- Bachiri, Najib (1)
- Bader, Michael (1)
- Badesso, Brian (1)
- Baer, Alexandra (1)
- Bahrenberg, Gregor (1)
- Baier, M. (1)
- Baitzel, Catherina (1)
- Balakrishnan, Karthikeyan (1)
- Balimane, Praveen (1)
- Ballemans, L. (1)
- Baltensperger, Urs (1)
- Balthasar, Nina (1)
- Baranyai, Zsuzsa (1)
- Barbazán, Paula (1)
- Bart, Hans-Jörg (1)
- Bartsch, J. (1)
- Bartz, Alexander (1)
- Barve, Avantika (1)
- Bauch, Melanie (1)
- Baumann, M. (1)
- Bayer, Hendrik (1)
- Becht, Alexander (1)
- Beck-Sickinger, Annette G. (1)
- Becker, C. (1)
- Beckers, Mario (1)
- Begemann, Jens (1)
- Beging, Stefan (1)
- Behner, T. (1)
- Beier, Reinhold (1)
- Belgardt, Bengt F. (1)
- Beloqui, Ana (1)
- Benham, C. (1)
- Berg, G. (1)
- Berg, Joost H. van den (1)
- Bergmann, Martin (1)
- Bergs, Michel (1)
- Betsch, Matthias (1)
- Bilban, Martin (1)
- Blader, Patrick (1)
- Bleisteiner, Monika (1)
- Block, Franziska (1)
- Blümich, B. (1)
- Bochert, Ulrich K. (1)
- Bode, J. (1)
- Boggetti, Barbara (1)
- Bolognani, Federico (1)
- Bonatelli, Maria (1)
- Booij, A. S. (1)
- Borgmeier, Claudia (1)
- Bork, M. (1)
- Bosch, L. (1)
- Bott, L. (1)
- Bouabid, Rachid (1)
- Bouhassira, Eric E. (1)
- Boulikas, T. (1)
- Bourioug, Mohamed (1)
- Bouwman, Peter (1)
- Bower, Conrad C. (1)
- Brabant, Georg (1)
- Brandini, F. (1)
- Brandini, Frederico (1)
- Brandt, Jens van den (1)
- Brasa, Sarah (1)
- Braun, Heiko (1)
- Braun, Thomas (1)
- Bremer, Erhard (1)
- Breuer, L. (1)
- Brock, Matthias (1)
- Brocke, Rainer (1)
- Bruhn, H. (1)
- Bruno, C. G. (1)
- Brönneke, Hella S. (1)
- Brückner, Benjamin (1)
- Brügger, M. (1)
- Brüser, Thomas (1)
- Buchholz, Heinrich (1)
- Buck, Manfred (1)
- Buckel, W. (1)
- Budianto, Arif (1)
- Bujnicki, R. (1)
- Bujnicki, Robert (1)
- Burdakov, Denis (1)
- Busch, Diana (1)
- Busch, Dirk H. (1)
- Böhmig, Georg A. (1)
- Böttger, Thomas (1)
- Büchs, J. (1)
- Bühl, Michael (1)
- Büllesbach, Erika E. (1)
- Caldeira, Sandra (1)
- Calzada-Wack, Julia (1)
- Capitain, C. (1)
- Cappellen, Wiggert A. van (1)
- Carballo, Rosa (1)
- Casanova, F. (1)
- Casotti, R. (1)
- Castillo, J. (1)
- Cehreli, Ruksan (1)
- Cereghini, Silvia (1)
- Cesari, Francesca (1)
- Chandra, Amita (1)
- Chasseigneaux, Stephanie (1)
- Chayeb, Hamid (1)
- Chen, Ruijiu (1)
- Chitnis, Ajay B. (1)
- Chowdhury, Nilanjan Pal (1)
- Christoph, Thomas (1)
- Cirpus, Irina (1)
- Claßen, Melanie (1)
- Clegg, William (1)
- Coppari, Roberto (1)
- Cornelissen-Steijger, Paulien (1)
- Corvinus, Florian M. (1)
- Cronin, Neil J. (1)
- Croué, Jean-Philippe (1)
- Cwienczek, Marcel (1)
- Dahl, Christiane (1)
- Dahmen, Markus (1)
- Danho, Waleed (1)
- Darley, Dan J. (1)
- Darley, Daniel (1)
- Davinson, T. (1)
- De Gooijer, Cornelis D. (1)
- Declèves, Xavier (1)
- Deflon, Victor M. (1)
- Degering, Christian (1)
- Dehairs, F. (1)
- Dell, Kerstin (1)
- Delle, L. (1)
- Dellmann, Sophia Florence (1)
- Demmer, Julius K. (1)
- Denkova, A.G. (1)
- Denkova, Antonia G. (1)
- Denzler, Hans-Jörg (1)
- Deterding, A. (1)
- Dhiman, Shashi K. (1)
- DiLoreto, Karen (1)
- Dickel, T. (1)
- Dickie, A. P. (1)
- Dickie, A.P. (1)
- Diehl, Bernd (1)
- Diehl, Bernd W. (1)
- Diehm, Ralf (1)
- Dierks, T. (1)
- Dietrich, Sascha (1)
- Digel, Ilya (1)
- Dillmann, Iris (1)
- Dimitrova, Darina (1)
- Ding, Xinxin (1)
- Dinter, Andre (1)
- Dmytriev, D. (1)
- Dodel, Richard (1)
- Dong, Lihua (1)
- Dreisbach, C. (1)
- Drost, Rinske (1)
- Dubost, Valerie (1)
- Dubost, Valérie (1)
- Dunker, R. (1)
- Dursun, Gözde (1)
- Dutta, Suryendu (1)
- Duwe, Anna (1)
- Dünkelmann, Pascal (1)
- E-Refai, Ahmed (1)
- Eberhardt, R. (1)
- Eberhardt, Rolf (1)
- Ebert, Klaus H. (1)
- Eck, J. (1)
- Ederer, Hanns J. (1)
- Eggert, Thorsten (1)
- Ehrenreich, Armin (1)
- Eisen, Andrew (1)
- El Bergui, Omnia (1)
- El Hatka, Hicham (1)
- El Moussaoui, Noureddine (1)
- Ellinger-Ziegelbauer, Heidrung (1)
- Engels, Ralf (1)
- Englberger, Werner (1)
- Englert, Christoph (1)
- Engudar, Gokce (1)
- Epple, Matthias (1)
- Erbacher, P. (1)
- Erbay, E. (1)
- Erdmann, Volker A. (1)
- Ermler, Ulrich (1)
- Esposito, Irene (1)
- Esser, Simon (1)
- Evers, Raymond (1)
- Faath, Stefan (1)
- Fagroud, Mustapha (1)
- Fedotova, Elena S. (1)
- Fekete, Csaba (1)
- Feliks, Mikolaj (1)
- Feng, Yong-Qing (1)
- Fischer, David (1)
- Fischer, Rainer (1)
- Flaig, Sascha T. (1)
- Foerstel, D. H. (1)
- Forouzan, Dara (1)
- Forstner, Oliver (1)
- Frahm, J. (1)
- Franke, Dirk (1)
- Frech, Moritz (1)
- Freire-Fernández, D. (1)
- Freudl, Roland (1)
- Freyschmidt, Eva-Jasmin (1)
- Friedrich, Andrea (1)
- Friedrich, Peter (1)
- Friedrich, W. (1)
- Friesel, Jürgen (1)
- Frink, C. (1)
- Fuchs, Helmut (1)
- Föhles, Joseph (1)
- Förster, Arnold (1)
- Gabor, E. (1)
- Gabriel, B. (1)
- Gailus-Durner, Valérie (1)
- Galgens, Jochem (1)
- Ganpo-Nkwenkwa, E. (1)
- Garske, U. (1)
- Gebeshuber, Christoph A. (1)
- Geier, Ethan G. (1)
- Geissel, H. (1)
- Geissler, Miriam (1)
- Gelissen, Melanie (1)
- Gemünde, Andre (1)
- Gensior, A. (1)
- Georgopanos, P. (1)
- Gerlach, J. (1)
- Ghod, Mumin M. (1)
- Ghosch, S. (1)
- Gieselmann, Volkmar (1)
- Gissmann, Lutz (1)
- Giudicelli, Fanny (1)
- Glaser, Stefan (1)
- Gleitz, M. (1)
- Glorius, J. (1)
- Glöggler, S. (1)
- Goebel, H. [u.a.] (1)
- Goetze, Sandra (1)
- Goeyens, L. (1)
- Goga, N. (1)
- Gokarn, Ravi R. (1)
- Gomez, Raquel (1)
- Gomez-Zepeda, David (1)
- Gonzalez, Laura Osorio (1)
- Goodman, Jay (1)
- Goodman, S. D. (1)
- Gottrup, Marcus (1)
- Goubeaud, Marcel (1)
- Goßmann, Matthias (1)
- Grankin, Alina (1)
- Grasl-Kraupp, Bettina (1)
- Grefen, Dana (1)
- Greger, M. (1)
- Gregorich, K. E. (1)
- Greiner, Lasse (1)
- Griffin, Christopher J. (1)
- Grisenti, R. (1)
- Groettrup, Marcus (1)
- Grogg, A. (1)
- Grondal, Christoph (1)
- Grootegoed, J. Anton (1)
- Grosse, J. O. (1)
- Grosse, Joachim O. (1)
- Grossmann, Matthias (1)
- Groth, Anne (1)
- Groudine, Mark (1)
- Grömping, Markus (1)
- Gröne, Hermann-Josef (1)
- Grützke, Martin (1)
- Guebitz, G. M. (1)
- Guenther, Klaus (1)
- Gulden, Hanneke van der (1)
- Gumberidze, Alexandre (1)
- Guo, Jiaqi (1)
- Guo, Yuanyuan (1)
- Gyngell, M. L. (1)
- Gäb, Martina (1)
- Gätgens, J. (1)
- Göbel, K. (1)
- Günther, Klaus (1)
- Haanen, J. B. A. G. (1)
- Haanen, John B. (1)
- Haettner, Emma (1)
- Hafidi, Youssef (1)
- Hagenbach, Adelheid (1)
- Hagmann, Siegbert (1)
- Hahne, Florian (1)
- Halbach, Thorsten (1)
- Hallmann, Armin (1)
- Hambach, B. (1)
- Hammon, U. (1)
- Hammon, Ulrich (1)
- Handge, U. A. (1)
- Hanicke, W. (1)
- Hannig, M. (1)
- Hans, Marcus (1)
- Hara, Hisanori (1)
- Harish, Ajay B. (1)
- Harrington, Ross W. (1)
- Hartkopf-Fröder, Christoph (1)
- Hasegawa, Maki (1)
- Haumaitre, Cécile (1)
- Hayward, Michael D. (1)
- He, Handan (1)
- Heard, David J. (1)
- Heeren, Joerg (1)
- Heering, J. (1)
- Heider, Johann (1)
- Heijden, Ingrid van der (1)
- Heil, M. (1)
- Heilmann, Marcel (1)
- Heimann, Cornelia (1)
- Heimbach, Tycho (1)
- Heine, A. (1)
- Heinze, D. (1)
- Heinze, Daniel (1)
- Heinzle, Elmar (1)
- Hemmerling, H.-J. (1)
- Hemmerling, Hans-Jörg (1)
- Hendo, Karsten (1)
- Hengsbach, Jan-Niklas (1)
- Henken, F. E. (1)
- Hennek, Thomas (1)
- Herfurth, Cornelia (1)
- Hering, T. (1)
- Hering, Thomas (1)
- Hermann, Gloria (1)
- Herold, Marco J. (1)
- Herrera, Cony N. (1)
- Herrmann, G. (1)
- Herrmanns, Karolin (1)
- Hertel, Robert (1)
- Heschel, I. (1)
- Heumann, S. (1)
- Heß, R. (1)
- Heßberger, F. P. (1)
- Hijum, Sacha A. F. T. van (1)
- Hillenbrand, P.-M. (1)
- Hirche, H.-J. (1)
- Hirsch, E. (1)
- Hitzeroth, Inga I. (1)
- Hodenius, M. A. J. (1)
- Hoeijmakers, Jan H. J. (1)
- Hoepfner, Sandra (1)
- Hoffmann, Alexander (1)
- Hoffmann, Katharina (1)
- Hoffmann, Tamara (1)
- Hoft, Carolin (1)
- Hogervorst, Frank B. L. (1)
- Holighaus, G. (1)
- Holzgrabe, Ulrike (1)
- Hooijberg, E. (1)
- Horvath, Tamas L. (1)
- Hough, Lindsay B. (1)
- Howell, Nicholas R. (1)
- Hrabe de Angelis, Martin (1)
- Hruschka, Steffen M. (1)
- Hubberten, D.N. (1)
- Huck, Christina (1)
- Hummel, Helga (1)
- Hummel, Joana (1)
- Hunger, K. (1)
- Huster, Manuel (1)
- Huth, A. (1)
- Hörstermann, D. (1)
- Höxter, Maria (1)
- Iber, M. (1)
- Ignatova, Ludmila (1)
- Ihme, B. (1)
- Iken, Heiko (1)
- Immel, Timo (1)
- Immel, Timo A. (1)
- Inagaki, Akiko (1)
- Infantino, Angelo (1)
- Irlenbusch, Sigrid (1)
- Ittobane, Najim (1)
- Jackson, Tim (1)
- Jacobi, M. (1)
- Jaenisch, Rudolf (1)
- Janke, Heinz Peter (1)
- Janoschek, Ruth (1)
- Jeitner, C. (1)
- Jelinek, Nanni (1)
- Jessen, Holly (1)
- Joksch, Burkhard (1)
- Jomaa, Ahlem (1)
- Jordan, Sabine D. (1)
- Joseph, R. (1)
- Jungk, Juliane (1)
- Jurado, B. (1)
- Jäger, E. (1)
- Kahlcke, Nils (1)
- Kahn, C. Ronald (1)
- Kahnt, Jörg (1)
- Kain, Renate (1)
- Kalteis, Magdalena (1)
- Kampeis, Percy (1)
- Kappel, Sven (1)
- Kappler-Tanudyaya, Nathalie (1)
- Karimi-Varnaneh, Hossein Ali (1)
- Kashkar, Hamid (1)
- Kassmi, Khalil (1)
- Katsifis, Andrew G. (1)
- Kattner, G. (1)
- Kaufmann, Andreas M. (1)
- Kaufmann, R. (1)
- Kayser, Peter (1)
- Kehler, Jonathan (1)
- Kenyon, George L. (1)
- Kerjaschki, Dontscho (1)
- Ketterer, Lothar (1)
- Kiesewetter, André (1)
- Kim, Ceol-Hee (1)
- Kim, Jihoe (1)
- Kirschbaum, M. (1)
- Kistaubayeva, Aida (1)
- Klatte, Stephanie (1)
- Kleinridders, Andre (1)
- Klijn, Christiaan N. (1)
- Klingenspor, Martin (1)
- Kloock, Joachim P. (1)
- Klose, Holger (1)
- Klostermeyer, Henning (1)
- Klyushnichenko, Vadim (1)
- Knecht, Rainald (1)
- Kneen, Malea M. (1)
- Knippschild, Uwe (1)
- Koch, Sabine (1)
- Kohn, Sophie (1)
- Kolter-Jung, Doris (1)
- Kong, Shushu (1)
- Konow, Christopher (1)
- Kornauth, Christoph (1)
- Kornfeld, Jan-Wilhelm (1)
- Kotnik, Katarina (1)
- Kovacs, J. (1)
- Kozak, Stefan (1)
- Kozhuharov, Christophor (1)
- Kranert, W. T. (1)
- Kranz, Andrea (1)
- Kratz, F. (1)
- Krauss, N. (1)
- Kreft, Bertolt (1)
- Kremer, Susanne (1)
- Kreutzer, A. (1)
- Kroll, Stephen (1)
- Krone, Wilhelm (1)
- Krueger, Rolf (1)
- Krueger, Sylvia (1)
- Krämer, Andrea (1)
- Krüger, Markus (1)
- Krüger, Peter (1)
- Kuhne, Stefan (1)
- Kula, M.-R. (1)
- Kulikov, I. (1)
- Kumar, G. (1)
- Kumar, Vivekanantha (1)
- Kurreck, Jens (1)
- Kurunci-Csacsko, Elisabeth (1)
- Kurutsch, Anja (1)
- Kuthan, Kai (1)
- Kuwada, John Y. (1)
- Kögel, Babette (1)
- Kückmann, Theresa (1)
- Küppers, Tobias (1)
- Lacayo, Jorge (1)
- Lammers, Tom (1)
- Lancelot, C. (1)
- Lange, H. R. (1)
- Lange, J. (1)
- Lange, M. (1)
- Langer, Christoph (1)
- Langer, Oliver (1)
- Langsdorf, Alexander (1)
- Lara, R.J. (1)
- Lawson, Nathan D. (1)
- Le, Dao (1)
- Leber, R. (1)
- Leboulch, Philippe (1)
- Leckenby, Guy (1)
- Lederer-Woods, C. (1)
- Lee, Chris Jaeil (1)
- Lee, Dongtao N. (1)
- Leitner, W. (1)
- Leitner, Walter (1)
- Lengkeek, Nigel (1)
- Lenzen, Sigurd (1)
- Lestinsky, M. (1)
- Li, D. X. (1)
- Li, Wenkui (1)
- Liebl, Wolfgang (1)
- Liese, Andreas (1)
- Lin, Wen (1)
- Linden, Gottfried (1)
- Lingen, Bettina (1)
- Linnemann, J. (1)
- Lisdat, Fred (1)
- Lisinski, Susanne (1)
- Litvinov, S. A. (1)
- Litvinov, Yu A. (1)
- Liu, Z. (1)
- Lobe, Melanie (1)
- Lohuizen, Maarten van (1)
- Lorbach, Volker (1)
- Lorenz, B. A. (1)
- Lorenz, E. (1)
- Loss, Peter (1)
- Loveland, Kate L. (1)
- Lowis, Carsten (1)
- Lubitz, Sandra (1)
- Lukatela, G. (1)
- Längle, Ulrich (1)
- Löher, Bastian (1)
- Lüdemann, H. D. (1)
- Ma, Xiaosong (1)
- Maass, Karin (1)
- Maaß, D. (1)
- Machnik, Marc (1)
- Maggakis-Kelemen, C. (1)
- Maier, G. (1)
- Mak, Tak W. (1)
- Mamaril-Fishman, Donna (1)
- Mandawe, John (1)
- Mann, Ulrich (1)
- Marcellin, Magali (1)
- Mariano, K. (1)
- Markert, Bernd (1)
- Marlowe, Jennifer (1)
- Marquard, Andrea (1)
- Marquardt, Andreas (1)
- Marsh, J. (1)
- Martinez, Javier (1)
- Martins, Berta M. (1)
- Marz, Martin (1)
- Marzábal, Pablo (1)
- Matoni, Georg (1)
- Matthess, Yves (1)
- Maul, F. D. (1)
- Maurer, Jan (1)
- May, Alexander (1)
- Mayer, Jan (1)
- McEwan, Jillian (1)
- McLeish, Michael J. (1)
- McNeil, S. W. (1)
- Mclaren, Aileen W. (1)
- Medlin, L. K. (1)
- Meier, Rahel (1)
- Meissner, Petra (1)
- Meißner, P. (1)
- Menet, Marie-Claude (1)
- Menz, Esther Babette (1)
- Merboldt, K. D. (1)
- Merboldt, K.-D. (1)
- Merkwirth, Carsten (1)
- Merseburg, Johannes (1)
- Methner, F.-J. (1)
- Metzger, Ute (1)
- Meyer, Hanna (1)
- Meyer, Jan (1)
- Meyer, Lina (1)
- Meyers, Ann E. (1)
- Mezo, Gabor (1)
- Miao, Qing (1)
- Michalak, Ewa Malgorzata (1)
- Michel, Nico (1)
- Miech, Claudia (1)
- Mielke, C. (1)
- Milow, Barbara (1)
- Miyamoto, Ko-ichiro (1)
- Moeller, Marcus J. (1)
- Mojsen-Moeller, Jens (1)
- Molina, R. (1)
- Molina, Roberto (1)
- Moll, Fabian (1)
- Monzon, Magaly (1)
- Morawiec, Laurent (1)
- Moret, J.L.T.M. (1)
- Morgenroth, T. (1)
- Mori, Marcelo A. (1)
- Moriggl, Richard (1)
- Muntel, Jan (1)
- Musina, Kristina T. (1)
- Mußmann, Nina (1)
- Möhring, Sophie (1)
- Möller, Martin (1)
- Mühlsiepen, Heinz (1)
- Müller, Bettina (1)
- Müller, Janina (1)
- Müller, Joachim (1)
- Müller, Michael A. (1)
- Müller-Renno, C.M. (1)
- Müller-Renno, Christine (1)
- Münstermann, Felix (1)
- Münzberg, Heike (1)
- Müschen, S. (1)
- Nacerddine, Karim (1)
- Nachtrodt, Frederik (1)
- Naithani, V. K (1)
- Naithani, Vinod K. (1)
- Nalwalk, Julia W. (1)
- Nehls, Jenny (1)
- Nehlsen, Kristina (1)
- Netz, Daili Jacqueline Aguilar (1)
- Neuner, Andreas (1)
- Ngyugen, Hung Huy (1)
- Nicholls, Hayley T. (1)
- Niedermeyer, Angela (1)
- Nieger, Martin (1)
- Niehaus, F. (1)
- Nikolaev, Lev (1)
- Nitsche, Adam (1)
- Noack, Udo (1)
- Noor, Johan A.E. (1)
- Nordheim, Alfred (1)
- Nork, Jasmina (1)
- Näser, Ulrike (1)
- Oehlenschläger, Katharina (1)
- Oertel, Dan (1)
- Ohbo, Kazu (1)
- Ojovan, Michael I. (1)
- Olkkonen, Vesa M. (1)
- Ommen, J.R. van (1)
- Ooms, Marja (1)
- Oosterhuis, K. (1)
- Oosterhuis, Koen (1)
- Orf, Sonja (1)
- Orth, Carina (1)
- Oswald, Stefan (1)
- Pasteur, Aline (1)
- Pawlitzky, Inka (1)
- Peiler, Tanja (1)
- Pellegrini, Paul (1)
- Pereira, Tony (1)
- Pes, Michaela (1)
- Peter, Karin (1)
- Petridis, N. (1)
- Pettrak, Jürgen (1)
- Pfeffer, Hans-Ulrich (1)
- Pham, Van N. (1)
- Pibernat, Jerome (1)
- Picard, Frank (1)
- Pierre, Esaie (1)
- Pieterse, Mark (1)
- Pietersen, Alexandra (1)
- Plummer, Simon M. (1)
- Pogrebnjak, Alexander (1)
- Pohl, Regula (1)
- Polen, T. (1)
- Popescu, C. (1)
- Popova, Elena (1)
- Popp, U. (1)
- Portela-Klein, Theresa (1)
- Poth, Sebastian (1)
- Prasad, Bhagwat (1)
- Prasetyanti, Pramudita (1)
- Prevost, David (1)
- Prinz, Florian (1)
- Prissok, F. (1)
- Probst, Johanna (1)
- Psaltis, Athanasios (1)
- Pude, Ralf (1)
- Puls, Michael (1)
- Putzer, Harald (1)
- Raab, Monika (1)
- Rachinger, Michael (1)
- Radchenko, V. (1)
- Rahba, Jade (1)
- Rajewsky, Klaus (1)
- Rakowski, D. (1)
- Rath, S. (1)
- Rathkolb, Birgit (1)
- Ratke, Lorenz (1)
- Raupp, Sebastian M. (1)
- Rechmann, Hanrik (1)
- Recker, Carla (1)
- Recker, Inga (1)
- Recksiek, Michael (1)
- Redemann, Nora (1)
- Regestein, Lars (1)
- Reichardt, Holger M. (1)
- Reifarth, R. (1)
- Reinecke, Diana (1)
- Reiss, Martina (1)
- Rennekampff, Verena (1)
- Rettner, Cornelius (1)
- Reugels, Alexander M. (1)
- Reuter, K. (1)
- Riesner, Detlev (1)
- Rio-Espinola, Alberto del (1)
- Ritterbecks, S. (1)
- Robb, Lorraine (1)
- Rodenas, J. (1)
- Rodenbrock, Anja (1)
- Rodriguez-Hermida, Sabina (1)
- Roellig, Daniela (1)
- Rohde, M. (1)
- Roikaew, W. (1)
- Roikaew, Wipa (1)
- Rolef, G. (1)
- Romuli, Sebastian (1)
- Roosen, C. (1)
- Roosen, Christoph (1)
- Rosman, Jan (1)
- Ross-Jones, J. (1)
- Ross-Jones, Jesse (1)
- Rother, Eva (1)
- Rousseau, V. (1)
- Rudolph, Tobias (1)
- Ruth, Thomas (1)
- Ruth, Thomas J. (1)
- Rutledge, Douglas N. (1)
- Rybicki, Edward P. (1)
- Rädle, M. (1)
- Röhl, Thomas (1)
- Rösch, C. (1)
- Saalwächter, Kay (1)
- Sagol, Ozgul (1)
- Sakshaug, E. (1)
- Salpati, Laurent (1)
- Salphati, Laurent (1)
- Sanhaji, Mourad (1)
- Sanjari, Shahab (1)
- Santana, H. H. S. (1)
- Sasaki, André N. (1)
- Sauter, Sybille L. (1)
- Savitskaya, Irina (1)
- Schaap, K. S. (1)
- Schabel, Wilhelm (1)
- Scharfer, Philip (1)
- Scheele, Sandra (1)
- Scheidenberger, C. (1)
- Scheidt, Annette (1)
- Scheja, Ludger (1)
- Schenk, Frieder (1)
- Scheper, Thomas (1)
- Scherbichler, Nina (1)
- Schiedermeier, Maximilian (1)
- Schiene, Klaus (1)
- Schimpf, E. (1)
- Schinkel, Alfred H. (1)
- Schlake, Thomas (1)
- Schlegel, Christin (1)
- Schlüter, Rabea (1)
- Schmich, Peter (1)
- Schmid, Georg (1)
- Schmidt, Aaron C. (1)
- Schmidt, Sebastian (1)
- Schmitt, Heike (1)
- Schmitt, Marcel (1)
- Schmitz, M. (1)
- Schmitz, Sandra (1)
- Schmitz-Rode, T. (1)
- Schmoll, H.-J. (1)
- Schneeweiss, F. (1)
- Schneider, Benno (1)
- Schollmayer, Curd (1)
- Schoor, Michael (1)
- Schröder, Bernd (1)
- Schröder, Wolfgang (1)
- Schulcz, Akos (1)
- Schumacher, Katharina (1)
- Schumacher, Ton N. (1)
- Schusser, Sebastian (1)
- Schwab, Lukas (1)
- Schwarz, E. (1)
- Schwarzer, Klemens (1)
- Schweighöfer, Philip V. (1)
- Schübeler, D. (1)
- Schürmann, Melanie (1)
- Schütz, J. (1)
- Scott, Richard (1)
- Sehr, Peter (1)
- Severins, Robin (1)
- Seynnes, Olivier R. (1)
- Sguazzin, M. (1)
- Shanabrough, Marya (1)
- Shepherd, Rachael K. (1)
- Shima, Seigo (1)
- Shin, Myung K. (1)
- Sidhu, Ragandeep Singh (1)
- Sierig, Ralph (1)
- Sievers, Susanne (1)
- Simic, Damir (1)
- Singh, Arjun (1)
- Singh, Tejvir (1)
- Singh, Vivendra (1)
- Singla, M. L. (1)
- Singla, Madan L. (1)
- Sleddens-Linkels, Esther (1)
- Sloboshanin, S. (1)
- Smith, Walker O. (1)
- Snaith, Mike (1)
- Snezhkov, Evgeney (1)
- Soars, Matthew G. (1)
- Sommerlade, Hans-Jörg (1)
- Sorich, Maren (1)
- Spelthahn, Heiko (1)
- Spies, Elmar (1)
- Spiess, Elmar (1)
- Spillmann, Uwe (1)
- Spohn, U. (1)
- Spohn, Uwe (1)
- Späte, Anne-Katrin (1)
- Stadtmüller, Ralf (1)
- Stanley, Lesley (1)
- Staubes, Regina (1)
- Steck, M. (1)
- Steenbergen, R. D. M. (1)
- Steffen, Victoria (1)
- Steinberg, Thorsten (1)
- Steinmetz, Hans-Jürgen (1)
- Steinwachs, S. (1)
- Stellberg, Michael (1)
- Stewart, A. Francis (1)
- Stoffel, Markus (1)
- Strebhardt, Klaus (1)
- Streu, Sandra (1)
- Strittmatter, Axel (1)
- Strobel, M. (1)
- Stöhlker, T. (1)
- Stückmann, Henning (1)
- Sumper, Manfred (1)
- Surzhykov, A. (1)
- Suzuki, Akira (1)
- Swartz, J. A. (1)
- Sänger, Nicole (1)
- Tahara, Harunobu (1)
- Talbi, Sofian (1)
- Talipova, Aizhan (1)
- Tanger, Ellen (1)
- Tauber, Stefanie (1)
- Tejeda, Miguel (1)
- Templin, Markus F. (1)
- Ternes, D. (1)
- Terranova, Remi (1)
- Terranova, Rémi (1)
- Terwesten, F. (1)
- Teumer, T. (1)
- Thamer, Wiebke (1)
- Theil, Diethilde (1)
- Theil, Dietlinde (1)
- Theis, K. (1)
- Thoelen, Ronald (1)
- Thomas, D. (1)
- Thomas, D.N. (1)
- Thömmes, Jörg (1)
- Tietsch, Wolfgang (1)
- Tillmann, Urban (1)
- Tim, Sieker (1)
- Tix, Julian (1)
- Todiras, Mihail (1)
- Toebes, Mireille (1)
- Tomasberger, T. (1)
- Trachtmann, Natalie (1)
- Tran, Quang Hon (1)
- Trapp, Svenja (1)
- Trautmann, S. (1)
- Treindl, Fridolin (1)
- Trimm, D. L. (1)
- Tu, Ran (1)
- Turek, Monika (1)
- Turek, Thomas (1)
- Turgut, Hatice (1)
- Törnqvist, H. (1)
- Ullmann, Matthias G. (1)
- Umanskaya, N. (1)
- Unadkat, Jashvant D. (1)
- Unterberger, Elif B. (1)
- Upcraft, T.M. (1)
- Vahidpour, Farnoosh (1)
- Vardag, T. (1)
- Varga, L. (1)
- Vaulot, D. (1)
- Veltkamp, T. C. (1)
- Vescovi, Diego (1)
- Vintersten, Kristina (1)
- Vitobello, Antonio (1)
- Vitry, Veronique (1)
- Vlad, Dorina (1)
- Vogel, Ortwin (1)
- Vogt, Merly C. (1)
- Vogt, Thomas F. (1)
- Voigt, B. (1)
- Volkmar, Marianne (1)
- Voß, Leonie (1)
- Vry, Jean de (1)
- Vuong, Lam Giang (1)
- Vázquez-López, Ezequiel M. (1)
- Völkering, Georg (1)
- Wackwitz, B. (1)
- Wagner, Michaela (1)
- Wagner, Patrick Hermann (1)
- Wagner, Sebastian (1)
- Waller, Mark P. (1)
- Walz, Anna-Lena (1)
- Wambach, M. (1)
- Wanek, Thomas (1)
- Wang, J. (1)
- Wang, Ren-Qi (1)
- Wang, Shijun (1)
- Wardoyo, Arinto Y.P. (1)
- Warren, J.T. (1)
- Wassenaar, Evelyne (1)
- Weichold, O. (1)
- Weichold, Oliver (1)
- Weick, H. (1)
- Weidner, Uwe (1)
- Weigand, M. (1)
- Weinstein, Brant M. (1)
- Weizäcker, Elisabeth von (1)
- Welden, Rene (1)
- Welsch, Volker (1)
- Wendisch, V. F. (1)
- Wendlandt, Tim (1)
- Werkhausen, Amelie (1)
- Westerman, Karen A. (1)
- Wetzel, Katharina (1)
- Weyland, F. (1)
- Weyland, Friedrich (1)
- Whitehead, Mark (1)
- Wiebel, Franziska F. (1)
- Wiechert, Wolfgang (1)
- Wielbalck, Swantje (1)
- Wilfried, Reichardt (1)
- Wilhelm, Manfred (1)
- Willanzheimer, Angela (1)
- Willenberg, H. (1)
- Willenberg, Holger S. (1)
- Willmes, Diana M. (1)
- Wilming, Anja (1)
- Wilson, C. E. (1)
- Wilson, Claire E. (1)
- Wilson, David L. (1)
- Wilson, I. D. (1)
- Winckler, Silvia (1)
- Winkelmann, Gunda (1)
- Winkelmann, Silke (1)
- Wissenbach, U. (1)
- Witte, Karin (1)
- Wolf, Anna M. (1)
- Wolf, Charles Roland (1)
- Wolf, Eckhard (1)
- Wolterbeek, H.T. (1)
- Woods, P. (1)
- Woznyj, M. (1)
- Wriggers, Peter (1)
- Wright, Jayne (1)
- Wrzodek, Clemens (1)
- Wulfhorst, Helene (1)
- Wunderlich, H. (1)
- Wunderlich, Hartmut (1)
- Xian, Mo (1)
- Xing, Y. (1)
- Yamaguchi, Taiyo (1)
- Yang, Hua (1)
- Yazici, Y. (1)
- Yazici, Yasemen (1)
- Yegen, Eda (1)
- Yu, Lihua (1)
- Yuan, Juping (1)
- Zahn, H. (1)
- Zamurovic, Natasa (1)
- Zauner, Stefan (1)
- Zell, Andreas (1)
- Zeng, Steffen (1)
- Zhang, Jin (1)
- Zhang, Xiaolin (1)
- Zhantlessova, Sirina (1)
- Zhou, Bei (1)
- Ziegler, C. (1)
- Ziegler, Christiane (1)
- Zientz, Evelyn (1)
- Zollinger, Tulipan (1)
- Zoor, Annegreth (1)
- Zoschke, Sascha (1)
- Zou, Huibin (1)
- [u.a.] (1)
- de Blois, E. (1)
- de Kruijff, Robin M. (1)
- van Waterschoot, Robert A.B. (1)
Year of publication
Institute
- Fachbereich Chemie und Biotechnologie (472) (remove)
Language
- English (472) (remove)
Document Type
- Article (472) (remove)
Keywords
- Heparin (3)
- Bacillaceae (2)
- Biotechnological application (2)
- Butanol (2)
- Chemometrics (2)
- IR spectroscopy (2)
- NMR spectroscopy (2)
- Principal component analysis (2)
- Standardization (2)
- Subtilases (2)
The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.
Bäcker, Matthias ; Beging, Stefan ; Biselli, Manfred ; Poghossian, Arshak ; Wang, J. ; Zang, Werner ; Wagner, Patrick ; Schöning, Michael Josef
Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.
Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988
(1988)
Cytochrome b5 Is a Major Determinant of Human Cytochrome P450 CYP2D6 and CYP3A4 Activity In Vivo s
(2015)
Scheer, Nico ; Kapelyukh, Yury ; Rode, Anja ; Oswald, Stefan ; Busch, Diana ; Mclaughlin, Lesley A. ; Lin, De ; Henderson, Colin J. ; Wolf, C. Roland
Scheer, Nico ; Mclaughlin, Lesley A. ; Rode, Anja ; MacLeod, Alastair Kenneth ; Henderson, Colin J. ; Wolf, Roland C.
In humans, 75% of all drugs are metabolized by the cytochrome P450-dependent monooxygenase system. Enzymes encoded by the CYP2C, CYP2D, and CYP3A gene clusters account for ∼80% of this activity. There are profound species differences in the multiplicity of cytochrome P450 enzymes, and the use of mouse models to predict pathways of drug metabolism is further complicated by overlapping substrate specificity between enzymes from different gene families. To establish the role of the hepatic and extrahepatic P450 system in drug and foreign chemical disposition, drug efficacy, and toxicity, we created a unique mouse model in which 30 cytochrome P450 genes from the Cyp2c, Cyp2d, and Cyp3a gene clusters have been deleted. Remarkably, despite a wide range of putative important endogenous functions, Cyp2c/2d/3a KO mice were viable and fertile, demonstrating that these genes have evolved primarily as detoxification enzymes. Although there was no overt phenotype, detailed examination showed Cyp2c/2d/3a KO mice had a smaller body size (15%) and larger livers (20%). Changes in hepatic morphology and a decreased blood glucose (30%) were also noted. A five-drug cocktail of cytochrome P450 isozyme probe substrates were used to evaluate changes in drug pharmacokinetics; marked changes were observed in either the pharmacokinetics or metabolites formed from Cyp2c, Cyp2d, and Cyp3a substrates, whereas the metabolism of the Cyp1a substrate caffeine was unchanged. Thus, Cyp2c/2d/3a KO mice provide a powerful model to study the in vivo role of the P450 system in drug metabolism and efficacy, as well as in chemical toxicity.
Density reduction effects on the production of [11C]CO2 in Nb-body targets on a medical cyclotron
(2023)
Medical isotope production of 11C is commonly performed in gaseous targets. The power deposition of the proton beam during the irradiation decreases the target density due to thermodynamic mixing and can cause an increase of penetration depth and divergence of the proton beam. In order to investigate the difference how the target-body length influences the operation conditions and the production yield, a 12 cm and a 22 cm Nb-target body containing N2/O2 gas were irradiated using a 13 MeV proton cyclotron. It was found that the density reduction has a large influence on the pressure rise during irradiation and the achievable radioactive yield. The saturation activity of [11C]CO2 for the long target (0.083 Ci/μA) is about 10% higher than in the short target geometry (0.075 Ci/μA).
Hoffstadt, Kevin ; Cheenakula, Dheeraja ; Nikolausz, Marcell ; Krafft, Simone ; Harms, Hauke ; Kuperjans, Isabel
The increasing share of renewable electricity in the grid drives the need for sufficient storage capacity. Especially for seasonal storage, power-to-gas can be a promising approach. Biologically produced methane from hydrogen produced from surplus electricity can be used to substitute natural gas in the existing infrastructure. Current reactor types are not or are poorly optimized for flexible methanation. Therefore, this work proposes a new reactor type with a plug flow reactor (PFR) design. Simulations in COMSOL Multiphysics ® showed promising properties for operation in laminar flow. An experiment was conducted to support the simulation results and to determine the gas fraction of the novel reactor, which was measured to be 29%. Based on these simulations and experimental results, the reactor was constructed as a 14 m long, 50 mm diameter tube with a meandering orientation. Data processing was established, and a step experiment was performed. In addition, a kLa of 1 h−1 was determined. The results revealed that the experimental outcomes of the type of flow and gas fractions are in line with the theoretical simulation. The new design shows promising properties for flexible methanation and will be tested.