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Keywords
- biosensors (3)
- Bacillus atrophaeus (1)
- Bioabsorbable (1)
- Calorimetric gas sensor (1)
- Capacitive field-effect sensor (1)
- Dehydrogenase (1)
- Diaphorase (1)
- Enzymatic biosensor (1)
- Hydrogen peroxide (1)
- Label-free detection (1)
- O2 plasma (1)
- Plant virus (1)
- Polyimide (1)
- Polylactide acid (1)
- Resistive temperature detector (1)
- Silk fibroin (1)
- Simultaneous determination (1)
- Sterilisation process (1)
- TMV adsorption (1)
- Ta₂O₅ gate (1)
- Tobacco mosaic virus (TMV) (1)
- Zeta potential (1)
- acetoin (1)
- acetoin reductase (1)
- alcoholic beverages (1)
- amperometric biosensors (1)
- annealing (1)
- biocompatible (1)
- biocompatible materials (1)
- biodegradabl (1)
- biodegradable electronic devices (1)
- calorimetric gas sensor;hydrogen peroxide;wireless sensor system (1)
- capacitive field-effect sensor (1)
- capacitive field-effect sensors (1)
- carbon electrodes (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- encapsulation materials (1)
- endospores (1)
- fibroin (1)
- glucose (1)
- hydroxylation (1)
- immobilization (1)
- organosilanes (1)
- plant virus detection (1)
- silanization (1)
- surface functionalization (1)
- tobacco mosaic virus (TMV) (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (43) (remove)
An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.
Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta₂O₅-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta₂O₅-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles.