Refine
Year of publication
- 2012 (313) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (70)
- Fachbereich Wirtschaftswissenschaften (42)
- Fachbereich Chemie und Biotechnologie (39)
- Fachbereich Elektrotechnik und Informationstechnik (37)
- Fachbereich Bauingenieurwesen (30)
- INB - Institut für Nano- und Biotechnologien (30)
- IfB - Institut für Bioengineering (29)
- Fachbereich Maschinenbau und Mechatronik (27)
- Fachbereich Energietechnik (20)
- Solar-Institut Jülich (15)
Has Fulltext
- no (313) (remove)
Document Type
- Article (129)
- Conference Proceeding (81)
- Part of a Book (36)
- Book (26)
- Conference: Meeting Abstract (17)
- Patent (12)
- Doctoral Thesis (6)
- Report (3)
- Habilitation (1)
- Other (1)
Keywords
- (Bio)degradation (1)
- 3. EU Legislativpaket (1)
- 802.15.4 (1)
- Acceleration (1)
- Adsorption (1)
- Afterload (1)
- Alginate beads (1)
- Anastomotic leakage (1)
- Autolysis (1)
- Avalanche (1)
Risiken des Handels
(2012)
Aufbau von Handelseinheiten
(2012)
Stahlbetonbau
(2012)
Geotechnik
(2012)
Siedlungswasserwirtschaft
(2012)
Abfallwirtschaft
(2012)
Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of ‘gene-shuffled’ (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.
Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.
Wendehorst Beispiele aus der Baupraxis / Ulrich Vismann (Hrsg.). - 4., durchges. und aktualis. Aufl.
(2012)
Sensitivity of phase detection techniques in aerated chute flows to hydraulic design parameters
(2012)
Many tasks for autonomous agents or robots are best described by a specification of the environment and a specification of the available actions the agent or robot can perform. Combining such a specification with the possibility to imperatively program a robot or agent is what we call the actionbased imperative programming. One of the most successful such approaches is Golog. In this paper, we draft a proposal for a new robot programming language YAGI, which is based on the action-based imperative programming paradigm. Our goal is to design a small, portable stand-alone YAGI interpreter. We combine the benefits of a principled domain specification with a clean, small and simple programming language, which does not exploit any side-effects from the implementation language. We discuss general requirements of action-based programming languages and outline YAGI, our action-based language approach which particularly aims at embeddability.