Refine
Year of publication
- 2015 (301) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (70)
- Fachbereich Wirtschaftswissenschaften (42)
- IfB - Institut für Bioengineering (40)
- Fachbereich Elektrotechnik und Informationstechnik (36)
- INB - Institut für Nano- und Biotechnologien (36)
- Fachbereich Luft- und Raumfahrttechnik (32)
- Fachbereich Chemie und Biotechnologie (30)
- Fachbereich Bauingenieurwesen (27)
- Fachbereich Maschinenbau und Mechatronik (26)
- Fachbereich Energietechnik (23)
Document Type
- Article (126)
- Conference Proceeding (92)
- Part of a Book (39)
- Book (22)
- Other (7)
- Report (7)
- Doctoral Thesis (5)
- Patent (2)
- Part of a Periodical (1)
Keywords
- Attitude dynamics (1)
- Booster Station (1)
- Carsharing (1)
- Charging stations (1)
- Discrete Optimisation (1)
- Discrete Optimization (1)
- E-carsharing (1)
- E-mobility (1)
- Efficiency (1)
- Electrical vehicle (1)
- Energy (1)
- Fully connected car (1)
- Gamma distribution (1)
- Goodness-of-fit test (1)
- Gossamer structures (1)
- Hydraulic structures (1)
- Independence test (1)
- Inductive charging (1)
- Information and communication technology (1)
- Integrated mobility (1)
- Limit analysis (1)
- Mobility management (1)
- Multimode failure (1)
- Non-linear optimization (1)
- Open channels (1)
- Orbital dynamics (1)
- Parametric bootstrap (1)
- Passenger compartment (1)
- Physical modeling (1)
- Pump System (1)
- Reliability analysis (1)
- Shakedown analysis (1)
- Similitude (1)
- Solar sail (1)
- TGA (1)
- Technical Operations Research (TOR) (1)
- Thermal comfort (1)
- Thermal management (1)
- Urban areas (1)
- Vapnik–Čhervonenkis class (1)
- Ventilation System (1)
- asteroid lander (1)
- availability (1)
- best practice (1)
- building industry (1)
- building materials (1)
- designs and models (1)
- energy efficiency (1)
- energy transfer (1)
- engineering education (1)
- flotilla missions (1)
- gender and diversity (1)
- guide (1)
- heating system (1)
- iron and steel industry (1)
- materials technology (1)
- metal structure (1)
- mixed-integer linear programming (1)
- planetary defence (1)
- programming (1)
- renewable energy (1)
- research project (1)
- research report (1)
- resistance of materials (1)
- small spacecraft (1)
- solar sail (1)
- steel (1)
- sustainability (1)
- system optimization (1)
- system synthesis (1)
- teaching (1)
- technical operations research (1)
Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte–insulator–semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance–voltage and constant-capacitance measurements. In addition, the surface morphology of the PAH layer was studied by atomic force microscopy and contact-angle measurements. High hybridization signals of 34 and 43 mV were recorded in low-ionic strength solutions of 10 and 1 mM, respectively. In contrast, a small signal of 4 mV was recorded in the case of unspecific adsorption of fully mismatched ncDNA. The density of probe ssDNA and dsDNA molecules as well as the hybridization efficiency was estimated using the experimentally measured DNA immobilization and hybridization signals and a simplified double-layer capacitor model. The results of field-effect experiments were supported by fluorescence measurements, verifying the DNA-immobilization and hybridization event.
Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.
The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) inthe sample. In this study, a novel wound-healing assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the defect of a cell layer brought into proximity of the sensing surface.A reduced impedance inside the defect, which was artificially formed ina cell layer, was successfully visualized in a photocurrent image.