Article
Refine
Year of publication
- 2024 (16)
- 2023 (24)
- 2022 (34)
- 2021 (39)
- 2020 (44)
- 2019 (52)
- 2018 (47)
- 2017 (45)
- 2016 (32)
- 2015 (43)
- 2014 (42)
- 2013 (49)
- 2012 (41)
- 2011 (69)
- 2010 (61)
- 2009 (76)
- 2008 (51)
- 2007 (48)
- 2006 (32)
- 2005 (40)
- 2004 (75)
- 2003 (39)
- 2002 (45)
- 2001 (49)
- 2000 (53)
- 1999 (31)
- 1998 (32)
- 1997 (29)
- 1996 (27)
- 1995 (18)
- 1994 (9)
- 1993 (18)
- 1992 (11)
- 1991 (11)
- 1990 (15)
- 1989 (17)
- 1988 (20)
- 1987 (19)
- 1986 (6)
- 1985 (8)
- 1984 (7)
- 1983 (5)
- 1982 (20)
- 1981 (15)
- 1980 (29)
- 1979 (20)
- 1978 (25)
- 1977 (13)
- 1976 (16)
- 1975 (12)
- 1974 (4)
- 1973 (2)
- 1972 (6)
- 1968 (2)
- 1967 (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (1594) (remove)
Document Type
- Article (1594) (remove)
Keywords
- Einspielen <Werkstoff> (7)
- FEM (4)
- Finite-Elemente-Methode (4)
- LAPS (4)
- CellDrum (3)
- Label-free detection (3)
- biosensors (3)
- hydrogen peroxide (3)
- impedance spectroscopy (3)
- shakedown analysis (3)
- Bacillus atrophaeus (2)
- Bauingenieurwesen (2)
- Boundary integral equations (2)
- CAD (2)
- Capacitive field-effect sensor (2)
- Conductive boundary condition (2)
- Einspielanalyse (2)
- Empirical process (2)
- Field-effect sensor (2)
- Goodness-of-fit test (2)
- Independence test (2)
- Light-addressable potentiometric sensor (2)
- Lipopolysaccharide (2)
- Paired sample (2)
- Parametric bootstrap (2)
- Raman spectroscopy (2)
- Shakedown analysis (2)
- Stiffness (2)
- Traglastanalyse (2)
- Transmission eigenvalues (2)
- capacitive field-effect sensors (2)
- carbonized rice husk (2)
- civil engineering (2)
- constructive alignment (2)
- damage (2)
- examination (2)
- field-effect sensor (2)
- frequency mixing magnetic detection (2)
- likelihood ratio test (2)
- limit analysis (2)
- locomotion (2)
- long-term retention (2)
- multimodal (2)
- muscle fascicle behavior (2)
- not identically distributed (2)
- practical learning (2)
- shakedown (2)
- tobacco mosaic virus (TMV) (2)
- ultrasound (2)
- ultrasound imaging (2)
- (Bio)degradation (1)
- Acceleration (1)
- Achilles tendon (1)
- Adaptive control (1)
- Afterload (1)
- Ageing (1)
- Air purification (1)
- AlterG (1)
- Alternating plasticity (1)
- Alzheimer's disease (1)
- Analysis (1)
- Analytischer Zulaessigkeitsnachweis (1)
- Anastomotic leakage (1)
- Anatomy (1)
- Annulus Fibrosus (1)
- Architectural design (1)
- Architectural gear ratio (1)
- Architektur (1)
- Arthosetherapie (1)
- Assistive technology (1)
- Asymptotic efficiency (1)
- Aufschlagversuch (1)
- Autolysis (1)
- Automatic control (1)
- Axialbelastung (1)
- Axially cracked pipe (1)
- Bacillus atrophaeus spores (1)
- Bacillus sp (1)
- Bacterial cellulose (1)
- Balance (1)
- Basis Reduktion (1)
- Basis reduction (1)
- Bicharakteristikenverfahren (1)
- Bioabsorbable (1)
- Biocomposites (1)
- Biomechanical simulation (1)
- Bioreaktor (1)
- Biosolubilization (1)
- Blutzellenlagerung (1)
- Bone quality and biomechanics (1)
- Booster Station (1)
- Bootstrap (1)
- Bootstrapping (1)
- Boundary integral equations, (1)
- Brownian Pillow (1)
- CNOT (1)
- Calorimetric gas sensor (1)
- Capacitive field-effect (1)
- Capacitive model (1)
- Carbon sources (1)
- Cardiac myocytes (1)
- Cardiac tissue (1)
- Categorial variable (1)
- Cell permeability (1)
- Cellular force (1)
- Cellulose nanostructure (1)
- Cement infiltration (1)
- Cementoblast (1)
- Censored data (1)
- Chemical images (1)
- Chemical imaging sensor (1)
- Chemical sensor (1)
- Circular Dichroism (1)
- Clusterion (1)
- Co-managed care (1)
- Coal (1)
- Collective risk model (1)
- Competitiveness (1)
- Complex System (1)
- Complex-valued eigenvalues (1)
- Compliance (1)
- Compression (1)
- Computational biomechanics (1)
- Concomitant (1)
- Conductive Boundary Condition (1)
- Conservation laws (1)
- Constitutive model (1)
- Contractile tension (1)
- Contractility (1)
- Convex optimization (1)
- Cost-effectiveness (1)
- Coverage probability (1)
- Cramér-von-Mises statistic (1)
- Cramér-von-Mises test (1)
- Cross border adjustment mechanism (1)
- Crámer–von-Mises distance (1)
- Culture media (1)
- C–V method (1)
- DNA biosensor (1)
- DNA hybridization (1)
- DPA (dipicolinic acid) (1)
- Damage mechanics theory (1)
- Decomposition (1)
- Deep Learning (1)
- Deformation (1)
- Dehydrogenase (1)
- Design-by-analysis (1)
- Diaphorase (1)
- Dimensional splitting (1)
- Disc Degeneration (1)
- Discontinuous fractures (1)
- Discrete Optimization (1)
- Distorsion des oberen Sprunggelenks (1)
- Druckbeanspruchung (1)
- Druckbehälter (1)
- Druckbelastung (1)
- Druckgeräte (1)
- Drug simulation (1)
- E-Mobility (1)
- E. coli detection (1)
- EIS capacitive sensor (1)
- ELISA (1)
- ES-FEM (1)
- Eigenvalue trajectories (1)
- Einspiel-Analyse (1)
- Einspiel-Kriterium (1)
- Einspielen (1)
- Elastizität (1)
- Elderly (1)
- Electrolyte–insulator–semiconductor (1)
- Electromagnetism (1)
- Electromechanical modeling (1)
- Elektroenzephalographie (1)
- Elemental (1)
- End-to-end colorectal anastomosis (1)
- Endothelial cells (1)
- Endothelial dysfunction (1)
- Energy-intensive industry (1)
- Enterprise information systems (1)
- Entropy solution (1)
- Environmental impact (1)
- Enzymatic biosensor (1)
- Enzyme coverage (1)
- Enzyme logic gate (1)
- Enzyme-linked immunosorbent assay (1)
- Epithel (1)
- Equivalence test (1)
- Esophageal Doppler monitor (1)
- Experiment (1)
- Exponential Euler scheme, (1)
- Exponential time differencing (1)
- External knee adduction moments (1)
- FS-FEM (1)
- Fall prevention (1)
- Fault approximation (1)
- Fault detection (1)
- Fehlerstellen (1)
- Fibroblast (1)
- Field effect (1)
- Field-effect biosensor (1)
- Finite difference methods (1)
- Finite element analysis (1)
- Finite element modelling (1)
- Fließgrenze (1)
- Floor prices (1)
- Force (1)
- Forces (1)
- Fracture configuration (1)
- Fracture simulation (1)
- Freeze–thaw process (1)
- Frequency adaption (1)
- Frequency mixing magnetic detection (1)
- Functional Delta Method (1)
- Fußball (1)
- Gamma distribution (1)
- Geriatric (1)
- German (1)
- Glaucoma (1)
- Global and local collapse (1)
- Gold nanoparticles (1)
- Gonarthrose (1)
- Goodness-of-fit tests for uniformity (1)
- Grenzwertberechnung (1)
- Ground-level falls (1)
- H2 (1)
- Hadamard differentiability (1)
- Haemodialysis (1)
- Handbike (1)
- Harnleiter (1)
- Heart tissue culture (1)
- Helmholtz equation (1)
- Hemoglobin structure (1)
- Heterostructure (1)
- Higher-order codes (1)
- Hip fractures (1)
- Hodgkin–Huxley models (1)
- Hoeffding-Blum-Kiefer-Rosenblatt independence test (1)
- Homogenization (1)
- Hotelling’s T² test (1)
- Human-Computer interaction (1)
- Hydrogen peroxide (1)
- Hydrogenotrophic methanogens (1)
- Hämoglobin (1)
- Hämoglobinstruktur (1)
- Image Reconstruction (1)
- Impedance spectroscopy (1)
- Implicit methods (1)
- Incomplete data (1)
- Induced pluripotent stem cells (1)
- Information extraction (1)
- Inotropic compounds (1)
- Integrated empirical distribution (survival) function (1)
- Interior Neumann eigenvalues (1)
- Interior transmission eigenvalues (1)
- Interior transmission problem (1)
- Intervertebral Disc (1)
- Intradiscal Pressure (1)
- Inverse Scattering (1)
- Inverse dynamic problem (1)
- Inverse kinematic problem (1)
- Inverse scattering (1)
- Inverse scattering problem (1)
- Inverse spectral problem (1)
- Ion channels (1)
- Iterative learning control (1)
- Kinetic energy (1)
- Kniegelenkarthrose (1)
- Knochen (1)
- Knochenbildung (1)
- Knochenchirugie (1)
- Knochendichte (1)
- Kohlenstofffaser (1)
- LPS (1)
- Lab-on-Chip (1)
- Langevin theory (1)
- Layer-by-layer adsorption (1)
- LbL films (1)
- Light-addressable Potentiometric Sensor (1)
- Limit analysis (1)
- Liver (1)
- Long COVID (1)
- Luftreiniger (1)
- MBST (1)
- MCDA (1)
- MOS (1)
- Machine learning (1)
- Magnetic nanoparticles (1)
- Manipulated variables (1)
- Marginal homogeneity test (1)
- Master stamp (1)
- Materialermüdung (1)
- Matrix exponential (1)
- Mechanotransduction (1)
- Medusomyces gisevi (1)
- Metascintillator (1)
- Methane (1)
- Methanogenesis (1)
- Microcirculation (1)
- Micromagnetic simulation (1)
- Mild cognitive impairment (1)
- Mobility (1)
- Mobility tests (1)
- Mobility transition (1)
- Model-driven software engineering (1)
- Monotone methods (1)
- Multi-criteria decision analysis (1)
- Multi-dimensional partial differential equations (1)
- Multi-sample problem (1)
- Multianalyte detection (1)
- Multicell (1)
- Multiple TOF kernels (1)
- Multiplexing (1)
- Muscle (1)
- Muscle Fascicle (1)
- Muscle Force (1)
- Musculoskeletal model (1)
- Musculoskeletal system (1)
- Myocardial infarction and cardiac death (1)
- NONOate (1)
- Natural fibres (1)
- Negative impedance convertor (1)
- Neural Network (1)
- Nitric Oxide (1)
- Nitric Oxide Donor (1)
- Non-parallel fissures (1)
- Nonequilibrium dynamics (1)
- Nonlinear Dynamics (1)
- Nonlinear PDEs (1)
- Nonlinear eigenvalue problems (1)
- Nucleus Pulposus (1)
- Numerical inversion of Laplace transforms (1)
- Numerical linear algebra (1)
- Numerics (1)
- O2 plasma (1)
- Ocular blood flow (1)
- Organic light-emitting diode display (1)
- Organkultur (1)
- Osteoporose (1)
- Osteoporosis (1)
- Parabolic SPDEs (1)
- Paralympic sport (1)
- Penicillin (1)
- Permeability (1)
- Permeabilität (1)
- Peroxidase (1)
- Pflanzenphysiologie (1)
- Pflanzenscanner (1)
- Pflanzenstress (1)
- Pharmacology (1)
- Photolithographic mimics (1)
- Physiology (1)
- Plant virus (1)
- Plasmacluster ion technology (1)
- Plastizität (1)
- Poly(allylamine hydrochloride) (1)
- Poly(d,l-lacticacid) (1)
- Polyimide (1)
- Polylactide acid (1)
- Polymer-matrix composites (1)
- Post-COVID-19 syndrome (1)
- Potential theory (1)
- Preference assessment (1)
- Pressure loaded crack-face (1)
- Prevention (1)
- Product-integration (1)
- Progressive plastic deformation (1)
- Prophylaxis (1)
- Proximal humerus fracture (1)
- Psychiatrische Biomarker (1)
- Pulsations (1)
- Pump System (1)
- Quartz crystal microbalance (1)
- RVA (1)
- Ratchetting (1)
- Raumluft (1)
- Reaction-diffusion systems (1)
- Real distinct pole (1)
- Real-time monitoring (1)
- Recombinant activated protein C (1)
- Red blood cell storage (1)
- Regionalization (1)
- Rehabilitation Technology and Prosthetics (1)
- Rehabilitation engineering (1)
- Resampling test (1)
- Resistive temperature detector (1)
- Resolvent Operator (1)
- Resonance-mode measurement (1)
- Retinal vessel analysis (1)
- Retinal vessels (1)
- Robotic rehabilitation (1)
- Rohr (1)
- Rohrbruch (1)
- Rotator cuff (1)
- Running (1)
- S-FEM (1)
- ScaLAPACK (1)
- Schienbeinschoner (1)
- Schlafspindeldetektion (1)
- Schwammknochen (1)
- Semi-parametric random censorship model (1)
- Septic cardiomyopathy (1)
- Shakedown (1)
- Shakedown criterion (1)
- Silk fibroin (1)
- Simulation (1)
- Simultaneous determination (1)
- Skeletal muscle (1)
- Small Aral Sea (1)
- Sn₃O₄ (1)
- Software and systems modeling (1)
- Source term (1)
- Spleen (1)
- Sprunggelenkorthesen (1)
- Stahl (1)
- Steel industry (1)
- Sterilisation process (1)
- Stress concentrations (1)
- Strukturanalyse (1)
- SunRav BookEditor (1)
- Surface imprinted polymer (1)
- Surgical Navigation and Robotics (1)
- Surgical staplers (1)
- Survival analysis (1)
- TMV adsorption (1)
- TOF PET (1)
- Ta₂O₅ gate (1)
- Technical Operations Research (TOR) (1)
- Temperaturabhängigkeit (1)
- Tendon Rupture (1)
- Tendon properties (1)
- Tendons (1)
- Tension (1)
- Tinetti test (1)
- Tissue Engineering (1)
- Tobacco mosaic virus (TMV) (1)
- Traglast (1)
- Transmission Eigenvalues (1)
- Ultrasound (1)
- Uniaxial compression test (1)
- Vapnik–Čhervonenkis class (1)
- Variable height stapler design (1)
- Vascular response (1)
- Vasomotions (1)
- Velocity (1)
- Vertebroplastie (1)
- Vertebroplasty (1)
- Viscous flow (1)
- Viskose Strömung (1)
- Viskosität (1)
- Visual field asymmetry (1)
- Volterra integral equation (1)
- Volume of confidence regions (1)
- Volume status (1)
- Wolff's Law (1)
- Wolffsches Gesetz (1)
- Wundheilung (1)
- XOR (1)
- Zeta potential (1)
- Zug-Druck Belastung (1)
- acetoin (1)
- acetoin reductase (1)
- achilles tendon (1)
- actin cytoskeleton (1)
- adipose-derived stromal cells (ASCs) (1)
- adsorption (1)
- agility (1)
- alcoholic beverages (1)
- allocation (1)
- alternierend Verformbarkeit (1)
- amperometric biosensors (1)
- anaesthetic complications (1)
- anisotropy (1)
- ankle braces (1)
- ankle sprain (1)
- annealing (1)
- aortic perfusion (1)
- aquaculture (1)
- architecture (1)
- aromatic amines (1)
- arthrosis therapy (1)
- artificial olfactory image (1)
- asymptotic relative efficiency (1)
- atomic layer deposition (1)
- barium strontium titanate (1)
- biaxial tensile experiment (1)
- bicharacteristics (1)
- bioburdens (1)
- biocompatible (1)
- biocompatible materials (1)
- biodegradabl (1)
- biodegradable electronic devices (1)
- biomechanics (1)
- biopotential electrodes (1)
- biosensor (1)
- bone density (1)
- bone structure (1)
- bootstrap (1)
- burst pressure (1)
- burst tests (1)
- calorimetric gas sensor (1)
- calorimetric gas sensor;hydrogen peroxide;wireless sensor system (1)
- cancellous bone (1)
- capacitive electrolyte–insulator–semiconductor sensors (1)
- capacitive field-effect sensor (1)
- carbon electrodes (1)
- cardiomyocyte biomechanics (1)
- catalytic metal (1)
- cell aerosolization (1)
- cell atomization (1)
- central symmetry test (1)
- cerebral small vessel disease (1)
- chance constrained programming (1)
- chemical sensor (1)
- chip-based sensor setup (1)
- cognitive impairment (1)
- community dwelling (1)
- complete block symmetry (1)
- computational fluid dynamics analysis (1)
- conditional excess distribution (1)
- conditional expectation principle (1)
- confidence interval (1)
- connective tissue (1)
- constitutive modeling (1)
- contactless conductivity sensor (1)
- correlation (1)
- coupled Néel–Brownian relaxation dynamics (1)
- covariance principle (1)
- dental trauma (1)
- dialysis (1)
- difficult airway (1)
- direct method (1)
- distance learning (1)
- distorted element (1)
- double-lumen tube intubation (1)
- drop jump (1)
- e-books (1)
- e-issues (1)
- ecological structure (1)
- eigensolvers (1)
- elastic scattering (1)
- elastic solids (1)
- electrical conductivity of liquids (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- electromyography (1)
- electronic nose (1)
- encapsulation materials (1)
- endoluminal (1)
- endospores (1)
- energy absorption (1)
- energy dissipation (1)
- enzymatic (bio)degradation (1)
- enzymatic biosensor (1)
- enzyme cascade (1)
- epithelization (1)
- exchangeability test (1)
- extracorporeal membrane oxygenation (1)
- fatigue analyses (1)
- fibroin (1)
- fibulare Bandruptur (1)
- field-effect structure (1)
- flaw (1)
- force generation (1)
- forecast (1)
- forehead EEG (1)
- fortschreitende plastische Deformation (1)
- frequency mixing (1)
- functional data (1)
- gait (1)
- gas sensor (1)
- glucose (1)
- glucose oxidase (GOx) (1)
- gonarthrosis (1)
- goodness-of-fit test (1)
- graphene oxide (1)
- healthy aging (1)
- heavy metals (1)
- hiPS cardiomyocytes (1)
- high-intensity exercise (1)
- high-k material (1)
- horseradish peroxidase (HRP) (1)
- huge dimensional data (1)
- human dermal fibroblasts (1)
- humic acid (1)
- hydroxylation (1)
- hyper-gravity (1)
- hyperelastic (1)
- hypo-gravity (1)
- immobilization (1)
- in-ear EEG (1)
- in-situ monitoring (1)
- independence test (1)
- intraclass correlation coefficient (1)
- key performance indicators (1)
- konvexe Optimierung (1)
- lable-free detection (1)
- large language models (1)
- layer-by-layer technique (1)
- light-addressable potentiometric sensor (1)
- light-addressing technologies (1)
- lignite (1)
- limit load (1)
- lipopolysaccharide (1)
- lipopolysaccharides (1)
- load limit (1)
- magnetic actuation (1)
- magnetic beads (1)
- magnetic biosensing (1)
- magnetic nanoparticles (1)
- magnetic relaxation (1)
- magnetic sandwich immunoassay (1)
- magnetic sensing (1)
- magnetic separation (1)
- magnetic tweezers (1)
- magnetophoretic velocity (1)
- mechanical buffer (1)
- metagenomics (1)
- metal-oxide-semiconductor structure (1)
- method of fundamental solutions (1)
- microbial diversity (1)
- microfluidics (1)
- micromagnetic simulation (1)
- multi-functional material (1)
- multinomial distribution (1)
- multiparametric immunoassays (1)
- multivariate normal distribution (1)
- muscle mechanics (1)
- nanobelts (1)
- nanomaterials (1)
- nanostructured carbonized plant parts (1)
- nanostrukturierte carbonisierte Pflanzenteile (1)
- naphtols (1)
- non-simplex S-FEM elements (1)
- novel photoexcitation method (1)
- optical sensor setup (1)
- optical spore trapping (1)
- optical trapping (1)
- organosilanes (1)
- overload (1)
- pH sensors (1)
- parabolic flight (1)
- penicillin (1)
- penicillinase (1)
- performance analysis (1)
- performance testing (1)
- phenols (1)
- photoelectrochemistry (1)
- physiology (1)
- pipes (1)
- plant scanner (1)
- plant stress (1)
- plant virus detection (1)
- plug-based microfluidic device (1)
- poly(d, l-lactic acid) (1)
- polyaniline (1)
- portfolio risk (1)
- prevention (1)
- psychosocial (1)
- random effects (1)
- random effects meta-regression model (1)
- ratchetting (1)
- rehabilitation (1)
- reliability analysis (1)
- reliability of structures (1)
- retinal microvasculature (1)
- retinal vessels (1)
- running (1)
- rupture of the fibular ligament (1)
- sEMG (1)
- sarcomere operating length (1)
- scanned light pulse technique (1)
- semantic role labeling (1)
- separable Hilbert space (1)
- series elastic element behavior (1)
- shakedown analyses (1)
- shotgun sequencing (1)
- shoulder (1)
- silanization (1)
- simulation (1)
- smooth muscle contraction (1)
- softs (1)
- spatial resolution (1)
- speaker attribution (1)
- sprint start (1)
- standard error of measurement (1)
- sterilisation (1)
- sterility tests (1)
- sterilization (1)
- sterilization conditions (1)
- sterilization efficacy (1)
- sterilization methods (1)
- stiffness (1)
- stochastic programming (1)
- strain energy function (1)
- stretch reflex (1)
- stretch-shortening cycle (1)
- superparamagnetic bead (1)
- superparamagnetic nanoparticles (1)
- surface functionalization (1)
- surface modification (1)
- survival (1)
- temperature (1)
- tendon rupture (1)
- tension–torsion loading (1)
- test-retest reliability (1)
- thermal ratcheting (1)
- thermometry (1)
- tilted constant illumination (1)
- titanium dioxide photoanode (1)
- tri-lineage differentiation (1)
- turnip vein clearing virus (TVCV) (1)
- twin-fluid atomizer (1)
- ultrasonography (1)
- ultrathin gate insulators (1)
- uniformly most powerful invariant test (1)
- unloading (1)
- validation methods (1)
- vessels (1)
- videolaryngoscopy (1)
- virgin passive (1)
- viscoelasticity (1)
- visualization (1)
- walking (1)
- walking gait (1)
- wound healing (1)
- yield stress (1)
- · Psychiatrische Erkrankungen/Diagnostik (1)
Detecting synchronization clusters in multivariate time series via coarse-graining of Markov chains
(2007)
In this work, we present a compact, bifunctional chip-based sensor setup that measures the temperature and electrical conductivity of water samples, including specimens from rivers and channels, aquaculture, and the Atlantic Ocean. For conductivity measurements, we utilize the impedance amplitude recorded via interdigitated electrode structures at a single triggering frequency. The results are well in line with data obtained using a calibrated reference instrument. The new setup holds for conductivity values spanning almost two orders of magnitude (river versus ocean water) without the need for equivalent circuit modelling. Temperature measurements were performed in four-point geometry with an on-chip platinum RTD (resistance temperature detector) in the temperature range between 2 °C and 40 °C, showing no hysteresis effects between warming and cooling cycles. Although the meander was not shielded against the liquid, the temperature calibration provided equivalent results to low conductive Milli-Q and highly conductive ocean water. The sensor is therefore suitable for inline and online monitoring purposes in recirculating aquaculture systems.
Microbial diversity studies regarding the aquatic communities that experienced or are experiencing environmental problems are essential for the comprehension of the remediation dynamics. In this pilot study, we present data on the phylogenetic and ecological structure of microorganisms from epipelagic water samples collected in the Small Aral Sea (SAS). The raw data were generated by massive parallel sequencing using the shotgun approach. As expected, most of the identified DNA sequences belonged to Terrabacteria and Actinobacteria (40% and 37% of the total reads, respectively). The occurrence of Deinococcus-Thermus, Armatimonadetes, Chloroflexi in the epipelagic SAS waters was less anticipated. Surprising was also the detection of sequences, which are characteristic for strict anaerobes—Ignavibacteria, hydrogen-oxidizing bacteria, and archaeal methanogenic species. We suppose that the observed very broad range of phylogenetic and ecological features displayed by the SAS reads demonstrates a more intensive mixing of water masses originating from diverse ecological niches of the Aral-Syr Darya River basin than presumed before.
Background
Impairment of neurovascular coupling (NVC) was recently reported in the context of subarachnoid hemorrhage and may correlate with disease severity and outcome. However, previous techniques to evaluate NVC required invasive procedures. Retinal vessels may represent an alternative option for non-invasive assessment of NVC.
Methods
A prototype of an adapted retinal vessel analyzer was used to assess retinal vessel diameter in mice. Dynamic vessel analysis (DVA) included an application of monochromatic flicker light impulses in predefined frequencies for evaluating NVC. All retinae were harvested after DVA and electroretinograms were performed.
Results
A total of 104 retinal scans were conducted in 21 male mice (90 scans). Quantitative arterial recordings were feasible only in a minority of animals, showing an emphasized reaction to flicker light impulses (8 mice; 14 scans). A characteristic venous response to flicker light, however, could observed in the majority of animals. Repeated measurements resulted in a significant decrease of baseline venous diameter (7 mice; 7 scans, p < 0.05). Ex-vivo electroretinograms, performed after in-vivo DVA, demonstrated a significant reduction of transretinal signaling in animals with repeated DVA (n = 6, p < 0.001).
Conclusions
To the best of our knowledge, this is the first non-invasive study assessing murine retinal vessel response to flicker light with characteristic changes in NVC. The imaging system can be used for basic research and enables the investigation of retinal vessel dimension and function in control mice and genetically modified animals.
Delayed cerebral ischemia (DCI) is a common complication after aneurysmal subarachnoid hemorrhage (aSAH) and can lead to infarction and poor clinical outcome. The underlying mechanisms are still incompletely understood, but animal models indicate that vasoactive metabolites and inflammatory cytokines produced within the subarachnoid space may progressively impair and partially invert neurovascular coupling (NVC) in the brain. Because cerebral and retinal microvasculature are governed by comparable regulatory mechanisms and may be connected by perivascular pathways, retinal vascular changes are increasingly recognized as a potential surrogate for altered NVC in the brain. Here, we used non-invasive retinal vessel analysis (RVA) to assess microvascular function in aSAH patients at different times after the ictus.
Retinal Vessel Analysis (RVA) in the context of subarachnoid hemorrhage: A proof of concept study
(2016)
Background
Timely detection of impending delayed cerebral ischemia after subarachnoid hemorrhage (SAH) is essential to improve outcome, but poses a diagnostic challenge. Retinal vessels as an embryological part of the intracranial vasculature are easily accessible for analysis and may hold the key to a new and non-invasive monitoring technique. This investigation aims to determine the feasibility of standardized retinal vessel analysis (RVA) in the context of SAH.
Methods
In a prospective pilot study, we performed RVA in six patients awake and cooperative with SAH in the acute phase (day 2–14) and eight patients at the time of follow-up (mean 4.6±1.7months after SAH), and included 33 age-matched healthy controls. Data was acquired using a manoeuvrable Dynamic Vessel Analyzer (Imedos Systems UG, Jena) for examination of retinal vessel dimension and neurovascular coupling.
Results
Image quality was satisfactory in the majority of cases (93.3%). In the acute phase after SAH, retinal arteries were significantly dilated when compared to the control group (124.2±4.3MU vs 110.9±11.4MU, p<0.01), a difference that persisted to a lesser extent in the later stage of the disease (122.7±17.2MU, p<0.05). Testing for neurovascular coupling showed a trend towards impaired primary vasodilation and secondary vasoconstriction (p = 0.08, p = 0.09 resp.) initially and partial recovery at the time of follow-up, indicating a relative improvement in a time-dependent fashion.
Conclusion
RVA is technically feasible in patients with SAH and can detect fluctuations in vessel diameter and autoregulation even in less severely affected patients. Preliminary data suggests potential for RVA as a new and non-invasive tool for advanced SAH monitoring, but clinical relevance and prognostic value will have to be determined in a larger cohort.
On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis.
Humic substances originating from various organic matters can ameliorate soil properties, stimulate plant growth, and improve nutrient uptake. Due to the low calorific heating value, leonardite is rather unsuitable as fuel. However, it may serve as a potential source of humic substances. This study was aimed at characterizing the leonardite-based soil amendments and examining the effect of their application on the soil microbial community, as well as on potato growth and tuber yield. A high yield (71.1%) of humic acid (LHA) from leonardite has been demonstrated. Parental leonardite (PL) and LHA were applied to soil prior to potato cultivation. The 16S rRNA sequencing of soil samples revealed distinct relationships between microbial community composition and the application of leonardite-based soil amendments. Potato tubers were planted in pots in greenhouse conditions. The tubers were harvested at the mature stage for the determination of growth and yield parameters. The results demonstrated that the LHA treatments had a significant effect on increasing potato growth (54.9%) and tuber yield (66.4%) when compared to the control. The findings highlight the importance of amending leonardite-based humic products for maintaining the biogeochemical stability of soils, for keeping their healthy microbial community structure, and for increasing the agronomic productivity of potato plants.
The carbonized rice husk (CRH) was evaluated for its wound healing activity in rats using excision models. In this study, the influences of CRH on wound healing in rat skin in vivo and cellular behavior of human dermal fibroblasts in vitro were investigated. The obtained results showed that the CRH treatment promoted wound epithelization in rats and exhibited moderate inhibition of cell proliferation in vitro. CRH with lanolin oil treated wounds were found to epithelize faster as compared to controls.
The necessity of e-books as a primary of learning, its opportunities for realization of competence during training biologist and biotechnologist specialists are determined. Definitions and requirements to the e-books, its advantages in comparison with traditional textbooks, and the ways of creation of e-books in the SunRav BookEditor program are considered.
It was generally believed that coal sources are not favorable as live-in habitats for microorganisms due to their recalcitrant chemical nature and negligible decomposition. However, accumulating evidence has revealed the presence of diverse microbial groups in coal environments and their significant metabolic role in coal biogeochemical dynamics and ecosystem functioning. The high oxygen content, organic fractions, and lignin-like structures of lower-rank coals may provide effective means for microbial attack, still representing a greatly unexplored frontier in microbiology. Coal degradation/conversion technology by native bacterial and fungal species has great potential in agricultural development, chemical industry production, and environmental rehabilitation. Furthermore, native microalgal species can offer a sustainable energy source and an excellent bioremediation strategy applicable to coal spill/seam waters. Additionally, the measures of the fate of the microbial community would serve as an indicator of restoration progress on post-coal-mining sites. This review puts forward a comprehensive vision of coal biodegradation and bioprocessing by microorganisms native to coal environments for determining their biotechnological potential and possible applications.
Methane is a valuable energy source helping to mitigate the growing energy demand worldwide. However, as a potent greenhouse gas, it has also gained additional attention due to its environmental impacts. The biological production of methane is performed primarily hydrogenotrophically from H2 and CO2 by methanogenic archaea. Hydrogenotrophic methanogenesis also represents a great interest with respect to carbon re-cycling and H2 storage. The most significant carbon source, extremely rich in complex organic matter for microbial degradation and biogenic methane production, is coal. Although interest in enhanced microbial coalbed methane production is continuously increasing globally, limited knowledge exists regarding the exact origins of the coalbed methane and the associated microbial communities, including hydrogenotrophic methanogens. Here, we give an overview of hydrogenotrophic methanogens in coal beds and related environments in terms of their energy production mechanisms, unique metabolic pathways, and associated ecological functions.
There is a growing body of evidence for the effects of vitamin D on intestinal host-microbiome interactions related to gut dysbiosis and bowel inflammation. This brief review highlights the potential links between vitamin D and gut health, emphasizing the role of vitamin D in microbiological and immunological mechanisms of inflammatory bowel diseases. A comprehensive literature search was carried out in PubMed and Google Scholar using combinations of keywords “vitamin D,” “intestines,” “gut microflora,” “bowel inflammation”. Only articles published in English and related to the study topic are included in the review. We discuss how vitamin D (a) modulates intestinal microbiome function, (b) controls antimicrobial peptide expression, and (c) has a protective effect on epithelial barriers in the gut mucosa. Vitamin D and its nuclear receptor (VDR) regulate intestinal barrier integrity, and control innate and adaptive immunity in the gut. Metabolites from the gut microbiota may also regulate expression of VDR, while vitamin D may influence the gut microbiota and exert anti-inflammatory and immune-modulating effects. The underlying mechanism of vitamin D in the pathogenesis of bowel diseases is not fully understood, but maintaining an optimal vitamin D status appears to be beneficial for gut health. Future studies will shed light on the molecular mechanisms through which vitamin D and VDR interactions affect intestinal mucosal immunity, pathogen invasion, symbiont colonization, and antimicrobial peptide expression.
The scope of this study is the measurement of endotoxin adsorption rate for carbonized rice husk. It showed good adsorption properties for LPS. During the batch experiments, several techniques were used and optimized for improving the material’s adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics.
Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data
(2021)
The vast metabolic potential of microbes in brown coal (lignite) processing and utilization can greatly contribute to innovative approaches to sustainable production of high-value products from coal. In this study, the multi-faceted and complex coal biosolubilization process by Bacillus sp. RKB 7 isolate from the Kazakhstan coal-mining soil is reported, and the derived products are characterized. Lignite solubilization tests performed for surface and suspension cultures testify to the formation of numerous soluble lignite-derived substances. Almost 24% of crude lignite (5% w/v) was solubilized within 14 days under slightly alkaline conditions (pH 8.2). FTIR analysis revealed various functional groups in the obtained biosolubilization products. Analyses of the lignite-derived humic products by UV-Vis and fluorescence spectrometry as well as elemental analysis yielded compatible results indicating the emerging products had a lower molecular weight and degree of aromaticity. Furthermore, XRD and SEM analyses were used to evaluate the biosolubilization processes from mineralogical and microscopic points of view. The findings not only contribute to a deeper understanding of microbe–mineral interactions in coal environments, but also contribute to knowledge of coal biosolubilization and bioconversion with regard to sustainable production of humic substances. The detailed and comprehensive analyses demonstrate the huge biotechnological potential of Bacillus sp. for agricultural productivity and environmental health.
L.setulosus is offered for creation of biopreparation «Setulin», possesing he- mostatic action, the basic reactant of biopreparation is diterpen – lagochirzin. Results under the maintenance and dynamics of diterpen lagochirzin accumula- tion in various parts of L.setulosus are presented: in roots, stalks, leaves, flowers and calyx lobes during the growing season, and also results on conditions of cultivation L.setulosus in the conditions of a typical sierozem are resulted. From the obtained data is visible, that the given species of a plant is endemic. It is established, that dynamics of accumulation of lagochirzin in phytomass accrues from the beginning to the middle of the growing season. The chemical analysis of L.setulosus on a localization of lagochirzin in various organs of a plant, has shown, that the greatest quantity of lagochirzin collects in calyx lobes of the plants. Also it is established, that L.setulosus can be cultivated in the conditions of the typical sierozem, a mineral food is necessary for the given species of plants of Lagochilus genus, except nitric fertilizers. Comparative studying of wild-growing and cultural forms of L.setulosus has shown, that in the cultivated phytomass of plants the maintenance of lagochirzin on 17-20 % higher than in the wild-growing species.
The purpose of the current study was to examine the reproducibility of fascicle length and pennation angle of gastrocnemius medialis while human walking. To the best of our knowledge, this is the first study of the reproducibility of fascicle length and pennation angle of gastrocnemius medialis in vivo during human gait. Twelve males performed 10 gait trials on a treadmill, in 2 separate days. B-mode ultrasonography, with the ultrasound probe firmly adjusted in the transverse and frontal planes using a special cast, was used to measure the fascicle length and the pennation angle of the gastrocnemius medialis (GM). A Vicon 624 system with three cameras operating at 120 Hz was also used to record the ankle and knee joint angles. The results showed that measurements of fascicle length and pennation angle showed high reproducibility during the gait cycle, both within the same day and between different days. Moreover, the root mean square differences between the repeated waveforms of both variables were very small, compared with their ranges (fascicle length: RMS = ∼3 mm, range: 38–63 mm; pennation angle: RMS = ∼1.5°, range: 22–32°). However, their reproducibility was lower compared to the joint angles. It was found that representative data have to be derived by a wide number of gait trials (fascicle length ∼six trials, pennation angle more than 10 trials), to assure the reliability of the fascicle length and pennation angle in human gait.
For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.
The movement of magnetic beads due to a magnetic field gradient is of great interest in different application fields. In this report we present a technique based on a magnetic tweezers setup to measure the velocity factor of magnetically actuated individual superparamagnetic beads in a fluidic environment. Several beads can be tracked simultaneously in order to gain and improve statistics. Furthermore we show our results for different beads with hydrodynamic diameters between 200 and 1000 nm from diverse manufacturers. These measurement data can, for example, be used to determine design parameters for a magnetic separation system, like maximum flow rate and minimum separation time, or to select suitable beads for fixed experimental requirements.
In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles’ nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil’s signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic beads using frequency scanning in magnetic frequency mixing technique magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements.
Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection
(2019)
Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more
than 700 ng/ml.
Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.
Three-dimensional (3D) full-field measurements provide a comprehensive and accurate validation of finite element (FE) models. For the validation, the result of the model and measurements are compared based on two respective point-sets and this requires the point-sets to be registered in one coordinate system. Point-set registration is a non-convex optimization problem that has widely been solved by the ordinary iterative closest point algorithm. However, this approach necessitates a good initialization without which it easily returns a local optimum, i.e. an erroneous registration. The globally optimal iterative closest point (Go-ICP) algorithm has overcome this drawback and forms the basis for the presented open-source tool that can be used for the validation of FE models using 3D full-field measurements. The capability of the tool is demonstrated using an application example from the field of biomechanics. Methodological problems that arise in real-world data and the respective implemented solution approaches are discussed.