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Year of publication
Institute
- Fachbereich Medizintechnik und Technomathematik (1593) (remove)
Document Type
- Article (1593) (remove)
Keywords
- Einspielen <Werkstoff> (7)
- FEM (4)
- Finite-Elemente-Methode (4)
- LAPS (4)
- CellDrum (3)
- Label-free detection (3)
- biosensors (3)
- hydrogen peroxide (3)
- impedance spectroscopy (3)
- shakedown analysis (3)
Ciritsis, Alexander ; Horbach, Andreas ; Staat, Manfred ; Kuhl, Christiane K. ; Kraemer, Nils Andreas
Purpose
In vivo, a loss of mesh porosity triggers scar tissue formation and restricts functionality. The purpose of this study was to evaluate the properties and configuration changes as mesh deformation and mesh shrinkage of a soft mesh implant compared with a conventional stiff mesh implant in vitro and in a porcine model.
Material and Methods
Tensile tests and digital image correlation were used to determine the textile porosity for both mesh types in vitro. A group of three pigs each were treated with magnetic resonance imaging (MRI) visible conventional stiff polyvinylidene fluoride meshes (PVDF) or with soft thermoplastic polyurethane meshes (TPU) (FEG Textiltechnik mbH, Aachen, Germany), respectively. MRI was performed with a pneumoperitoneum at a pressure of 0 and 15 mmHg, which resulted in bulging of the abdomen. The mesh-induced signal voids were semiautomatically segmented and the mesh areas were determined. With the deformations assessed in both mesh types at both pressure conditions, the porosity change of the meshes after 8 weeks of ingrowth was calculated as an indicator of preserved elastic properties. The explanted specimens were examined histologically for the maturity of the scar (collagen I/III ratio).
Results
In TPU, the in vitro porosity increased constantly, in PVDF, a loss of porosity was observed under mild stresses. In vivo, the mean mesh areas of TPU were 206.8 cm2 (± 5.7 cm2) at 0 mmHg pneumoperitoneum and 274.6 cm2 (± 5.2 cm2) at 15 mmHg; for PVDF the mean areas were 205.5 cm2 (± 8.8 cm2) and 221.5 cm2 (± 11.8 cm2), respectively. The pneumoperitoneum-induced pressure increase resulted in a calculated porosity increase of 8.4% for TPU and of 1.2% for PVDF. The mean collagen I/III ratio was 8.7 (± 0.5) for TPU and 4.7 (± 0.7) for PVDF.
Conclusion
The elastic properties of TPU mesh implants result in improved tissue integration compared to conventional PVDF meshes, and they adapt more efficiently to the abdominal wall. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 827–833, 2018.
Christiaens, P. ; Abouzar, Maryam H. ; Poghossian, Arshak ; Wagner, Torsten ; Bijnens, N. ; Williams, O. A. ; Daenen, M. ; Haenen, K. ; Schöning, Michael Josef ; Wagner, P.
Christiaens, P. ; Abouzar, Maryam H. ; Poghossian, Arshak ; Wagner, Torsten ; Bijnens, N. ; Williams, O. A. ; Daenen, M. ; Haenen, K. ; Douthéret, O. ; Haen, J. d´ ; Mekhalif, Z. ; Schöning, Michael Josef ; Wagner, P.
Christ, D. ; Hollendung, A. ; Larue, H. ; Parl, C. ; Streun, M. ; Weber, S. ; Ziemons, Karl ; Halling, H.
MultiChannel Photomultipliers (PM), like the R7600-00-M64 or R5900-00-M64 from Hamamatsu, are often chosen as photodetectors in high-resolution positron emission tomography (PET). A major problem of this PM is the nonuniform channel gain. In order to solve this problem, light attenuating masks were created. The aim of the masks is a homogenization of the output of all 64 channels using different hole sizes at the channel positions. The hole area, which is individually defined for the different channels, is inversely proportional to the channel gain. The measurements by inserting light attenuating masks improved a homogenization to a ratio of 1:1.2.
Design, evaluation and comparison of endorectal coils for hybrid MR-PET imaging of the prostate
(2020)
Choi, Chang-Hoon ; Felder, Tim ; Felder, Jörg ; Tellmann, Lutz ; Hong, Suk-Min ; Wegener, Hans-Peter ; Shah, N Jon ; Ziemons, Karl
Prostate cancer is one of the most common cancers among men and its early detection is critical for its successful treatment. The use of multimodal imaging, such as MR-PET, is most advantageous as it is able to provide detailed information about the prostate. However, as the human prostate is flexible and can move into different positions under external conditions, it is important to localise the focused region-of-interest using both MRI and PET under identical circumstances. In this work, we designed five commonly used linear and quadrature radiofrequency surface coils suitable for hybrid MR-PET use in endorectal applications. Due to the endorectal design and the shielded PET insert, the outer face of the coils investigated was curved and the region to be imaged was outside the volume of the coil. The tilting angles of the coils were varied with respect to the main magnetic field direction. This was done to approximate the various positions from which the prostate could be imaged. The transmit efficiencies and safety excitation efficiencies from simulations, together with the signal-to-noise ratios from the MR images were calculated and analysed. Overall, it was found that the overlapped loops driven in quadrature were superior to the other types of coils we tested. In order to determine the effect of the different coil designs on PET, transmission scans were carried out, and it was observed that the differences between attenuation maps with and without the coils were negligible. The findings of this work can provide useful guidance for the integration of such coil designs into MR-PET hybrid systems in the future.
Chloé, Radermacher ; Malyaran, Hanna ; Craveiro, Rogerio Bastos ; Peglow, Sarah ; Behbahani, Mehdi ; Pufe, Thomas ; Wolf, Michael ; Neuss, Sabine
Orthodontic treatments are concomitant with mechanical forces and thereby cause teeth movements. The applied forces are transmitted to the tooth root and the periodontal ligaments which is compressed on one side and tensed up on the other side. Indeed, strong forces can lead to tooth root resorption and the crown-to-tooth ratio is reduced with the potential for significant clinical impact. The cementum, which covers the tooth root, is a thin mineralized tissue of the periodontium that connects the periodontal ligament with the tooth and is build up by cementoblasts. The impact of tension and compression on these cells is investigated in several in vivo and in vitro studies demonstrating differences in protein expression and signaling pathways. In summary, osteogenic marker changes indicate that cyclic tensile forces support whereas static tension inhibits cementogenesis. Furthermore, cementogenesis experiences the same protein expression changes in static conditions as static tension, but cyclic compression leads to the exact opposite of cyclic tension. Consistent with marker expression changes, the singaling pathways of Wnt/ß-catenin and RANKL/OPG show that tissue compression leads to cementum degradation and tension forces to cementogenesis. However, the cementum, and in particular its cementoblasts, remain a research area which should be explored in more detail to understand the underlying mechanism of bone resorption and remodeling after orthodontic treatments.
Chen, Chao ; Jost, Peter ; Volker, Hanno ; Kaminski, Marvin ; Wirtssohn, Matti R. ; Engelmann, Ulrich M. ; Krüger, K. ; Schlich, Franziska F. ; Schlockermann, Carl ; Lobo, Ricardo P.S.M. ; Wuttig, Matthias
Capri, Miriam ; Morsiani, Cristina ; Santoro, Aurelia ; Moriggi, Manuela ; Conte, Maria ; Martucci, Morena ; Bellavista, Elena ; Fabbri, Cristina ; Giampieri, Enrico ; Albracht, Kirsten ; Flück, Martin ; Ruoss, Severin ; Brocca, Lorenza ; Canepari, Monica ; Longa, Emanuela ; Giulio, Irene Di ; Bottinelli, Roberto ; Cerretelli, Paolo ; Salvioli, Stefano ; Gelfi, Cecilia ; Franceschi, Claudio ; Narici, Marco ; Rittweger, Jörn
Bäcker, Matthias ; Raue, Markus ; Schusser, Sebastian ; Jeitner, C. ; Breuer, L. ; Wagner, P. ; Poghossian, Arshak ; Förster, Arnold ; Mang, Thomas ; Schöning, Michael Josef
Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off.
Bäcker, Matthias ; Rakowski, D. ; Poghossian, Arshak ; Biselli, Manfred ; Wagner, Patrick ; Schöning, Michael Josef
A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.
Bäcker, Matthias ; Pouyeshman, S. ; Schnitzler, Thomas ; Poghossian, Arshak ; Wagner, Patrick ; Biselli, Manfred ; Schöning, Michael Josef
Bäcker, Matthias ; Poghossian, Arshak ; Schöning, Michael Josef ; Schnitzler, Thomas ; Biselli, Manfred ; Zang, Werner ; Wagner, P.
Bäcker, Matthias ; Poghossian, Arshak ; Abouzar, Maryam H. ; Wenmackers, Sylvia ; Janssens, Stoffel D. ; Haenen, Ken ; Wagner, Patrick ; Schöning, Michael Josef
Bäcker, Matthias ; Kramer, F. ; Huck, Christina ; Poghossian, Arshak ; Bratov, A. ; Abramova, N. ; Schöning, Michael Josef
Planar and three-dimensional (3D) interdigitated electrodes (IDE) with electrode digits separated by an insulating barrier of different heights were electrochemically characterized and compared in terms of their sensing properties. Due to the impact of the surface resistance, both types of IDE structures display a non-linear behavior in low-ionic strength solutions. The experimental data were fitted to an electrical equivalent circuit and interpreted taking into account the surface-charge-governed properties. The effect of a charged polyelectrolyte layer electrostatically assembled onto the sensor surface on the surface resistance in solutions with different KCl concentration is studied. In case of the same electrode footprint, 3D-IDEs show a larger cell constant and a higher sensitivity to molecular adsorption than that of planar IDEs. The obtained results demonstrate the potential of 3D-IDEs as a new transducer structure for a direct label-free sensing of charged molecules.
Bäcker, Matthias ; Kowollik, Silvia ; Schnitzler, Thomas ; Wang, J. ; Poghossian, Arshak ; Zang, Werner ; Biselli, Manfred ; Wagner, P. ; Schöning, Michael Josef
Bäcker, Matthias ; Koch, Claudia ; Eiben, Sabine ; Geiger, Fania ; Eber, Fabian ; Gliemann, Hartmut ; Poghossian, Arshak ; Wege, Christina ; Schöning, Michael Josef
The conjunction of (bio-)chemical recognition elements with nanoscale biological building blocks such as virus particles is considered as a very promising strategy for the creation of biohybrids opening novel opportunities for label-free biosensing. This work presents a new approach for the development of biosensors using tobacco mosaic virus (TMV) nanotubes or coat proteins (CPs) as enzyme nanocarriers. Sensor chips combining an array of Pt electrodes loaded with glucose oxidase (GOD)-modified TMV nanotubes or CP aggregates were used for amperometric detection of glucose as a model system for the first time. The presence of TMV nanotubes or CPs on the sensor surface allows binding of a high amount of precisely positioned enzymes without substantial loss of their activity, and may also ensure accessibility of their active centers for analyte molecules. Specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CPs was achieved via bioaffinity binding. These layouts were tested in parallel with glucose sensors with adsorptively immobilized [SA]-GOD, as well as [SA]-GOD crosslinked with glutardialdehyde, and came out to exhibit superior sensor performance. The achieved results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for future applications in biosensorics and biochips.
Bäcker, Matthias ; Delle, L. ; Poghossian, Arshak ; Biselli, Manfred ; Zang, Werner ; Wagner, P. ; Schöning, Michael Josef
Bäcker, Matthias ; Beging, Stefan ; Biselli, Manfred ; Poghossian, Arshak ; Wang, J. ; Zang, Werner ; Wagner, Patrick ; Schöning, Michael Josef
Reinigungsprozesse in der Lebensmittelindustrie. Entwicklung eines Demonstrators zur Überwachung
(2017)
Bussmann, N. ; Engels, R. ; Fuss, L. ; Kemmerling, G. ; Reinartz, R. ; Langen, K.-J. ; Schelten, J. ; Ziemons, Karl
A 2-dimensional detector system for high resolution thyroid I-131 scintigraphy was developed. It has a sensitive area of 4 cm×4 cm and consists of a lead-collimator and an array of 10×10 EGO crystals combined with a position sensitive photomultiplier. The spatial resolution and the sensitivity of the detector has been measured and compared to two commercially available gamma-cameras. Furthermore first patient measurements have been carried out
Burkhardt, Klaus ; Schwarz, Sonja ; Pan, Chengrui ; Stelter, Felix ; Kotliar, Konstantin ; Eynatten, Maxilian von ; Sollinger, Daniel ; Lanzl, Ines ; Heemann, Uwe ; Baumann, Marcus
Buniatyan, Vahe V. ; Matirosyan, N. ; Abouzar, Maryam H. ; Schubert, J. ; Zander, W. ; Gevorgian, S. ; Schöning, Michael Josef ; Poghossian, Arshak
Buniatyan, Vahe V. ; Abouzar, Maryam H. ; Martirosyan, Norayr W. ; Schubert, Jürgen ; Gevorgian, Spartak ; Schöning, Michael Josef ; Poghossian, Arshak
Buniatyan, V. V. ; Matirosyan, N. W. ; Abouzar, Maryam H. ; Schöning, Michael Josef ; Poghossian, Arshak ; Schubert, J. ; Khachatryan, V. R. ; Soukiassyan, G. R.
Buniatyan, V. ; Huck, Christina ; Poghossian, Arshak ; Aroutiounian, V. M. ; Schöning, Michael Josef
Buniatyan, V. ; Huck, Christina ; Poghossian, Arshak ; Aroutiounian, V. M. ; Schöning, Michael Josef
Bronder, Thomas ; Wu, Chunsheng ; Poghossian, Arshak ; Werner, Frederik ; Keusgen, M. ; Schöning, Michael Josef
Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).
Bronder, Thomas ; Poghossian, Arshak ; Scheja, Sabrina ; Wu, Chunsheng ; Keusgen, Michael ; Mewes, Dieter ; Schöning, Michael Josef
Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte–insulator–semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance–voltage and constant-capacitance measurements. In addition, the surface morphology of the PAH layer was studied by atomic force microscopy and contact-angle measurements. High hybridization signals of 34 and 43 mV were recorded in low-ionic strength solutions of 10 and 1 mM, respectively. In contrast, a small signal of 4 mV was recorded in the case of unspecific adsorption of fully mismatched ncDNA. The density of probe ssDNA and dsDNA molecules as well as the hybridization efficiency was estimated using the experimentally measured DNA immobilization and hybridization signals and a simplified double-layer capacitor model. The results of field-effect experiments were supported by fluorescence measurements, verifying the DNA-immobilization and hybridization event.
Bronder, Thomas ; Poghossian, Arshak ; Scheja, S. ; Wu, Chunsheng ; Keusgen, M. ; Schöning, Michael Josef
Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.
In this study, polyelectrolyte-modified field-effect-based electrolyte-insulator-semiconductor (EIS) devices have been used for the label-free electrical detection of double-stranded deoxyribonucleic acid (dsDNA)molecules. The sensor-chip functionalization with a positively charged polyelectrolyte layer provides the possibility of direct adsorptive binding of negatively charged target DNA oligonucleotides onto theSiO2-chip surface.EIS sensors can be utilized as a tool to detect surface-charge changes; the electrostatic adsorption of oligonucleotides onto the polyelectrolyte layer leads to a measureable surface-potential change. Signals of 39mV have been recorded after the incubation with the oligonucleotide solution. Besides the electrochemical experiments, the successful adsorption of dsDNA onto the polyelectrolyte layer has been verified via fluorescence microscopy. The presented results demonstrate that the signal recording of EISchips, which are modified with a polyelectrolyte layer, canbe used as a favorable approach for a fast, cheap and simple detection method for dsDNA.
Field-effect-based electrolyte-insulator-semiconductor (EIS) sensors were modified with a bilayer of positively charged weak polyelectrolyte (poly(allylamine hydrochloride) (PAH)) and probe single-stranded DNA (ssDNA) and are used for the detection of complementary single-stranded target DNA (cDNA) in different test solutions. The sensing mechanism is based on the detection of the intrinsic molecular charge of target cDNA molecules after the hybridization event between cDNA and immobilized probe ssDNA. The test solutions contain synthetic cDNA oligonucleotides (with a sequence of tuberculosis mycobacteria genome) or PCR-amplified DNA (which origins from a template DNA strand that has been extracted from Mycobacterium avium paratuberculosis-spiked human sputum samples), respectively. Sensor responses up to 41 mV have been measured for the test solutions with DNA, while only small signals of ∼5 mV were detected for solutions without DNA. The lower detection limit of the EIS sensors was ∼0.3 nM, and the sensitivity was ∼7.2 mV/decade. Fluorescence experiments using SybrGreen I fluorescence dye support the electrochemical results.
Brockhaus, Moritz K. ; Behbahani, Mehdi ; Muris, Farina ; Jansen, Sebastian V. ; Schmitz- Rode, Thomas ; Steinseifer, Ulrich ; Clauser, Johanna C.
Thrombogenic complications are a main issue in mechanical circulatory support (MCS). There is no validated in vitro method available to quantitatively assess the thrombogenic performance of pulsatile MCS devices under realistic hemodynamic conditions. The aim of this study is to propose a method to evaluate the thrombogenic potential of new designs without the use of complex in-vivo trials. This study presents a novel in vitro method for reproducible thrombogenicity testing of pulsatile MCS systems using low molecular weight heparinized porcine blood. Blood parameters are continuously measured with full blood thromboelastometry (ROTEM; EXTEM, FIBTEM and a custom-made analysis HEPNATEM). Thrombus formation is optically observed after four hours of testing. The results of three experiments are presented each with two parallel loops. The area of thrombus formation inside the MCS device was reproducible. The implantation of a filter inside the loop catches embolizing thrombi without a measurable increase of platelet activation, allowing conclusions of the place of origin of thrombi inside the device. EXTEM and FIBTEM parameters such as clotting velocity (α) and maximum clot firmness (MCF) show a total decrease by around 6% with a characteristic kink after 180 minutes. HEPNATEM α and MCF rise within the first 180 minutes indicate a continuously increasing activation level of coagulation. After 180 minutes, the consumption of clotting factors prevails, resulting in a decrease of α and MCF. With the designed mock loop and the presented protocol we are able to identify thrombogenic hot spots inside a pulsatile pump and characterize their thrombogenic potential.
In this article, a concept of implicit methods for scalar conservation laws in one or more spatial dimensions allowing also for source terms of various types is presented. This material is a significant extension of previous work of the first author (Breuß SIAM J. Numer. Anal. 43(3), 970–986 2005). Implicit notions are developed that are centered around a monotonicity criterion. We demonstrate a connection between a numerical scheme and a discrete entropy inequality, which is based on a classical approach by Crandall and Majda. Additionally, three implicit methods are investigated using the developed notions. Next, we conduct a convergence proof which is not based on a classical compactness argument. Finally, the theoretical results are confirmed by various numerical tests.
Breuer, Lars ; Raue, Markus ; Strobel, M. ; Mang, Thomas ; Schöning, Michael Josef ; Thoelen, R. ; Wagner, Torsten
Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.
Breuer, Lars ; Raue, Markus ; Kirschbaum, M. ; Mang, Thomas ; Schöning, Michael Josef ; Thoelen, R. ; Wagner, Torsten
Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
Breuer, Lars ; Pilas, Johanna ; Guthmann, Eric ; Schöning, Michael Josef ; Thoelen, Ronald ; Wagner, Torsten
Bressel, A. ; Schultze, J.W. ; Khan, W. ; Wolfaardt, G. M. ; Rohns, H.-P. ; Irmscher, R. ; Schöning, Michael Josef
The growing body of political texts opens up new opportunities for rich insights into political dynamics and ideologies but also increases the workload for manual analysis. Automated speaker attribution, which detects who said what to whom in a speech event and is closely related to semantic role labeling, is an important processing step for computational text analysis. We study the potential of the large language model family Llama 2 to automate speaker attribution in German parliamentary debates from 2017-2021. We fine-tune Llama 2 with QLoRA, an efficient training strategy, and observe our approach to achieve competitive performance in the GermEval 2023 Shared Task On Speaker Attribution in German News Articles and Parliamentary Debates. Our results shed light on the capabilities of large language models in automating speaker attribution, revealing a promising avenue for computational analysis of political discourse and the development of semantic role labeling systems.
Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro
(2013)
Bohrn, Ulrich ; Mucha, Andreas ; Werner, Frederik ; Trattner, Barbara ; Bäcker, Matthias ; Krumbe, Christoph ; Schienle, Meinrad ; Stütz, Evamaria ; Schmitt-Landsiedel, Doris ; Fleischer, Maximilian ; Wagner, Patrick ; Schöning, Michael Josef
Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas®), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security.