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Seifarth, Volker ; Grosse, Joachim O. ; Grossmann, Matthias ; Janke, Heinz Peter ; Arndt, Patrick ; Koch, Sabine ; Epple, Matthias ; Artmann, Gerhard ; Temiz Artmann, Aysegül
Seifarth, Volker ; Goßmann, Matthias ; Grosse, J. O. ; Becker, C. ; Heschel, I. ; Artmann, Gerhard ; Temiz Artmann, Aysegül
Seibler, Jost ; Zevnik, Branko ; Küter-Luks, Birgit ; Andreas, Susanne ; Kern, Heidrun ; Hennek, Thomas ; Rode, Anja ; Heimann, Cornelia ; Faust, Nicole ; Kauselmann, Gunther ; Schoor, Michael ; Jaenisch, Rudolf ; Rajewsky, Klaus ; Kühn, Ralf ; Schwenk, Frieder
Seibler, Jost ; Küter-Luks, Birgit ; Kern, Heidrun ; Streu, Sandra ; Plum, Leona ; Maurer, Jan ; Kühn, Ralf ; Brüning, Jens C. ; Schwenk, Frieder
Seibler, Jost ; Kleinridders, Andre ; Küter-Luks, Birgit ; Niehaves, Sandra ; Brüning, Jens C. ; Schwenk, Frieder
Rubber materials filled with reinforcing fillers display nonlinear rheological behavior at small strain amplitudes below γ0 < 0.1. Nevertheless, rheological data are analyzed mostly in terms of linear parameters, such as shear moduli (G′, G″), which loose their physical meaning in the nonlinear regime. In this work styrene butadiene rubber filled with carbon black (CB) under large amplitude oscillatory shear (LAOS) is analyzed in terms of the nonlinear parameter I3/1. Three different CB grades are used and the filler load is varied between 0 and 70 phr. It is found that I3/1(φ) is most sensitive to changes of the total accessible filler surface area at low strain amplitudes (γ0 = 0.32). The addition of up to 70 phr CB leads to an increase of I3/1(φ) by a factor of more than ten. The influence of the measurement temperature on I3/1 is pronounced for CB levels above the percolation threshold.
Schroeter, Rebecca ; Hoffmann, Tamara ; Voigt, Birgit ; Meyer, Hanna ; Bleisteiner, Monika ; Muntel, Jan ; Jürgen, Britta ; Albrecht, Dirk ; Becher, Dörte ; Lalk, Michael ; Evers, Stefan ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Putzer, Harald ; Hecker, Michael ; Schweder, Thomas ; Bremer, Erhard
The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.
The SG1-mediated solution polymerization of methyl methacrylate (MMA) and oligo(ethylene glycol) methacrylate (OEGMA, Mₙ = 300 g mol⁻¹) in the presence of a small amount of functional/reactive styrenic comonomer is investigated. Moieties such as pentafluorophenyl ester, triphenylphosphine, azide, pentafluorophenyl, halide, and pyridine are considered. A comonomer fraction as low as 5 mol% typically results in a controlled/living behavior, at least up to 50% conversion. Chain extensions with styrene for both systems were successfully performed. Variation of physical properties such as refractive index (for MMA) and phase transition temperature (for OEGMA) were evaluated by comparing to 100% pure homopolymers. The introduction of an activated ester styrene derivative in the polymerization of OEGMA allows for the synthesis of reactive and hydrophilic polymer brushes with defined thickness. Finally, using the example of pentafluorostyrene as controlling comonomer, it is demonstrated that functional PMMA-b-PS are able to maintain a phase separation ability, as evidenced by the formation of nanostructured thin films.
Detection and identification of free radicals in hydrocarbon pyrolysis by an iodine trapping method
(1992)
Schiffels, Johannes ; Pinkenburg, Olaf ; Schelden, Maximilian ; Aboulnaga, El-Hussiny A. A. ; Baumann, Marcus ; Selmer, Thorsten