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Praxistransfer in der tertiären Bildungsforschung: Modelle, Gelingensbedingungen und Nachhaltigkeit
(2019)
A review of guidelines and best practices for subsonic aerodynamic simulations using RANS CFD
(2019)
Flexible Fuel Operation of a Dry-Low-Nox Micromix Combustor with Variable Hydrogen Methane Mixtures
(2019)
Application of Low NOx Micro-mix Hydrogen Combustion to 2MW Class Industrial Gas Turbine Combustor
(2019)
Though weir flow has been studied for centuries, there still remains some nuances of weir flow that are not well understood. Therefore, an international study was conducted in which 20 different hydraulics laboratories from around the world built and tested two linear weirs (quarter-round and half-round crested weirs) of common geometry. The only unconstrained dimension was the weir length, which could be adjusted to match the width of the test flume. Participating laboratories used the instrumentation and data collection methodologies of their choosing for head and discharge measurements.
The experimental results found significant variability in the discharge coefficients as a function of dimensionless upstream head, as well as in the head-discharge relationships (as much as 50% in some cases). Potential sources contributing to the scatter may have included head meter instrumentation, flow meter instrumentation, approach flow length (flume length upstream of weir), head measurement location, nappe behavior, laboratory measurement methods and experimental setup, and the care and skill of the investigator (human error). Analyzing the data as a function of instrumentation types, approach length, and head measurement location did not provide any insight regarding the variations. Nappe behavior (e.g., aeration), which could be influenced by laboratory-specific conditions, varied among the datasets primarily for the half-round crested weir (about 20%).
Sabbioneta: Atlas
(2019)
Sensitivity Analysis of General Aviation Aircraft with Parallel Hybrid-Electric Propulsion Systems
(2019)
Laser-based Additive Manufacturing (AM) processes for the use of metals out of the powder bed have been investigated profusely and are prevalent in industry. Although there is a broad field of application, Laser Powder Bed Fusion (LPBF), also known as Selective Laser Melting (SLM) of glass is not fully developed yet. The material properties of glass are significantly different from the investigated metallic material for LPBF so far. As such, the process cannot be transferred, and the parameter limits and the process sequence must be redefined for glass. Starting with the characterization of glass powders, a parameter field is initially confined to investigate the process parameter of different glass powder using LPBFprocess. A feasibility study is carried out to process borosilicate glass powder. The effects of process parameters on the dimensional accuracy of fabricated parts out of borosilicate and hints for the post-processing are analysed and presented in this paper.
Heating efficiency of magnetic nanoparticles decreases with gradual immobilization in hydrogels
(2019)
Bacterial cell appendix formation supports cell-cell interaction, cell adhesion and cell movement. Additionally, in bioelectrochemical systems (BES), cell appendages have been shown to participate in extracellular electron transfer. In this work, the cell appendix formation of Clostridium acetobutylicum in biofilms of a BES are imaged and compared with conventional biofilms. Under all observed conditions, the cells possess filamentous appendages with a higher number and density in the BES. Differences in the amount of extracellular polymeric substance in the biofilms of the electrodes lead to the conclusion that the cathode can be used as electron donor and the anode as electron acceptor by C. acetobutylicum. When using conductive atomic force microscopy, a current response of about 15 nA is found for the cell appendages from the BES. This is the first report of conductivity for clostridial cell appendices and represents the basis for further studies on their role for biofilm formation and electron transfer.
Enzyme-catalyzed reactions have been designed to mimic various Boolean logic gates in the general framework of unconventional biomolecular computing. While some of the logic gates, particularly OR, AND, are easy to realize with biocatalytic reactions and have been reported in numerous publications, some other, like NXOR, are very challenging and have not been realized yet with enzyme reactions. The paper reports on a novel approach to mimicking the NXOR logic gate using the bell-shaped enzyme activity dependent on pH values. Shifting pH from the optimum value to the acidic or basic values by using acid or base inputs (meaning 1,0 and 0,1 inputs) inhibits the enzyme reaction, while keeping the optimum pH (assuming 0,0 and 1,1 input combinations) preserves a high enzyme activity. The challenging part of the present approach is the selection of an enzyme with a well-demonstrated bell-shape activity dependence on the pH value. While many enzymes can satisfy this condition, we selected pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase as this enzyme has the optimum pH center-located on the pH scale allowing the enzyme activity change by the acidic and basic pH shift from the optimum value corresponding to the highest activity. The present NXOR gate is added to the biomolecular “toolbox” as a new example of Boolean logic gates based on enzyme reactions.
Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.