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Zugriffsart
- weltweit (97)
- campus (48)
- bezahl (30)
- fachbereichsweit (FB4) (12)
06| Warum es gemeinsam besser geht
10| Interview
14| Wer ist hier der Boss?
18| Schnittstelle zwischen Mensch und Technik
22| Zweite Heimat Jülich
28| Zwischen Angst und Hoffnung
32| Eine Sternstunde für die FH Aachen
36| Gegen alle Widerstände
38| Ein Ort, der bleibt
42| Der Aufblühende
46| Der Computer sitzt am Steuer
52| Da geht das Herz auf
54| Hoch hinaus
58| Beratungsangebote
60| Das alte Schätzchen
In order to reduce energy consumption of homes, it is important to make transparent which devices consume how much energy. However, power consumption is often only monitored aggregated at the house energy meter. Disaggregating this power consumption into the contributions of individual devices can be achieved using Machine Learning. Our work aims at making state of the art disaggregation algorithms accessibe for users of the open source home automation platform Home Assistant.
Die Erfindung betrifft eine Vorrichtung zur Bestimmung einer Relativlage zwischen einem feststehenden Teil und einem zu demselben in eine Bewegungsrichtung bewegbaren beweglichen Teil, wobei der feststehende Teil mit einem Wiegandsensor versehen ist, wobei der Wiegandsensor zwischen zwei gegenpolig zueinander ausgebildeten Permanentmagneten angeordnet ist und dass der bewegliche Teil eine Mehrzahl von beabstandet zueinander angeordneten Magnetisierungsstegen aus einem magnetisch leitenden Material aufweist, die in der Bewegungsrichtung zumindest eine gleich große Erstreckung aufweisen wie der Permanentmagnet, dass ein Abstand zwischen benachbarten Magnetisierungsstegen derart gewählt ist, dass in einer ersten Relativlage ein erster Permanentmagnet von einem der Magnetisierungsstege überdeckt ist und ein zweiter Permanentmagnet nicht von einem der Magnetisierungsstege überdeckt ist.
Today’s society is undergoing a paradigm shift driven by the megatrend of sustainability. This undeniably affects all areas of Western life. This paper aims to find out how the luxury industry is dealing with this change and what adjustments are made by the companies. For this purpose, interviews were conducted with managers from the luxury industry, in which they were asked about specific measures taken by their companies as well as trends in the industry. In a subsequent evaluation, the trends in the luxury industry were summarized for the areas of ecological, social, and economic sustainability. It was found that the area of environmental sustainability is significantly more focused than the other sub-areas. Furthermore, the need for a customer survey to validate the industry-based measures was identified.
Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.
This thesis aims at the presentation and discussion of well-accepted and new
imaging techniques applied to different types of flow in common hydraulic
engineering environments. All studies are conducted in laboratory conditions and
focus on flow depth and velocity measurements. Investigated flows cover a wide
range of complexity, e.g. propagation of waves, dam-break flows, slightly and fully
aerated spillway flows as well as highly turbulent hydraulic jumps.
Newimagingmethods are compared to different types of sensorswhich are frequently
employed in contemporary laboratory studies. This classical instrumentation as well
as the general concept of hydraulic modeling is introduced to give an overview on
experimental methods.
Flow depths are commonly measured by means of ultrasonic sensors, also known as
acoustic displacement sensors. These sensors may provide accurate data with high
sample rates in case of simple flow conditions, e.g. low-turbulent clear water flows.
However, with increasing turbulence, higher uncertainty must be considered.
Moreover, ultrasonic sensors can provide point data only, while the relatively large
acoustic beam footprint may lead to another source of uncertainty in case of
relatively short, highly turbulent surface fluctuations (ripples) or free-surface
air-water flows. Analysis of turbulent length and time scales of surface fluctuations
from point measurements is also difficult. Imaging techniques with different
dimensionality, however, may close this gap. It is shown in this thesis that edge
detection methods (known from computer vision) may be used for two-dimensional
free-surface extraction (i.e. from images taken through transparant sidewalls in
laboratory flumes). Another opportunity in hydraulic laboratory studies comes with
the application of stereo vision. Low-cost RGB-D sensors can be used to gather
instantaneous, three-dimensional free-surface elevations, even in flows with very
high complexity (e.g. aerated hydraulic jumps). It will be shown that the uncertainty
of these methods is of similar order as for classical instruments.
Particle Image Velocimetry (PIV) is a well-accepted and widespread imaging
technique for velocity determination in laboratory conditions. In combination with
high-speed cameras, PIV can give time-resolved velocity fields in 2D/3D or even as
volumetric flow fields. PIV is based on a cross-correlation technique applied to small
subimages of seeded flows. The minimum size of these subimages defines the
maximum spatial resolution of resulting velocity fields. A derivative of PIV for
aerated flows is also available, i.e. the so-called Bubble Image Velocimetry (BIV). This
thesis emphasizes the capacities and limitations of both methods, using relatively
simple setups with halogen and LED illuminations. It will be demonstrated that
PIV/BIV images may also be processed by means of Optical Flow (OF) techniques.
OF is another method originating from the computer vision discipline, based on the
assumption of image brightness conservation within a sequence of images. The
Horn-Schunck approach, which has been first employed to hydraulic engineering
problems in the studies presented herein, yields dense velocity fields, i.e. pixelwise
velocity data. As discussed hereinafter, the accuracy of OF competes well with PIV
for clear-water flows and even improves results (compared to BIV) for aerated flow
conditions. In order to independently benchmark the OF approach, synthetic images
with defined turbulence intensitiy are used.
Computer vision offers new opportunities that may help to improve the
understanding of fluid mechanics and fluid-structure interactions in laboratory
investigations. In prototype environments, it can be employed for obstacle detection
(e.g. identification of potential fish migration corridors) and recognition (e.g. fish
species for monitoring in a fishway) or surface reconstruction (e.g. inspection of
hydraulic structures). It can thus be expected that applications to hydraulic
engineering problems will develop rapidly in near future. Current methods have not
been developed for fluids in motion. Systematic future developments are needed to
improve the results in such difficult conditions.
High aerodynamic efficiency requires propellers with high aspect ratios, while propeller sweep potentially reduces noise. Propeller sweep and high aspect ratios increase elasticity and coupling of structural mechanics and aerodynamics, affecting the propeller performance and noise. Therefore, this paper analyzes the influence of elasticity on forward-swept, backward-swept, and unswept propellers in hover conditions. A reduced-order blade element momentum approach is coupled with a one-dimensional Timoshenko beam theory and Farassat's formulation 1A. The results of the aeroelastic simulation are used as input for the aeroacoustic calculation. The analysis shows that elasticity influences noise radiation because thickness and loading noise respond differently to deformations. In the case of the backward-swept propeller, the location of the maximum sound pressure level shifts forward by 0.5 °, while in the case of the forward-swept propeller, it shifts backward by 0.5 °. Therefore, aeroacoustic optimization requires the consideration of propeller deformation.
Melting probes are a proven tool for the exploration of thick ice layers and clean sampling of subglacial water on Earth. Their compact size and ease of operation also make them a key technology for the future exploration of icy moons in our Solar System, most prominently Europa and Enceladus. For both mission planning and hardware engineering, metrics such as efficiency and expected performance in terms of achievable speed, power requirements, and necessary heating power have to be known.
Theoretical studies aim at describing thermal losses on the one hand, while laboratory experiments and field tests allow an empirical investigation of the true performance on the other hand. To investigate the practical value of a performance model for the operational performance in extraterrestrial environments, we first contrast measured data from terrestrial field tests on temperate and polythermal glaciers with results from basic heat loss models and a melt trajectory model. For this purpose, we propose conventions for the determination of two different efficiencies that can be applied to both measured data and models. One definition of efficiency is related to the melting head only, while the other definition considers the melting probe as a whole. We also present methods to combine several sources of heat loss for probes with a circular cross-section, and to translate the geometry of probes with a non-circular cross-section to analyse them in the same way. The models were selected in a way that minimizes the need to make assumptions about unknown parameters of the probe or the ice environment.
The results indicate that currently used models do not yet reliably reproduce the performance of a probe under realistic conditions. Melting velocities and efficiencies are constantly overestimated by 15 to 50 % in the models, but qualitatively agree with the field test data. Hence, losses are observed, that are not yet covered and quantified by the available loss models. We find that the deviation increases with decreasing ice temperature. We suspect that this mismatch is mainly due to the too restrictive idealization of the probe model and the fact that the probe was not operated in an efficiency-optimized manner during the field tests. With respect to space mission engineering, we find that performance and efficiency models must be used with caution in unknown ice environments, as various ice parameters have a significant effect on the melting process. Some of these are difficult to estimate from afar.
In this work, the effects of carbon sources and culture media on the production and structural properties of bacterial cellulose (BC) synthesized by Medusomyces gisevii have been studied. The culture medium was composed of different initial concentrations of glucose or sucrose dissolved in 0.4% extract of plain green tea. Parameters of the culture media (titratable acidity, substrate conversion degree etc.) were monitored daily for 20 days of cultivation. The BC pellicles produced on different carbon sources were characterized in terms of biomass yield, crystallinity and morphology by field emission scanning electron microscopy (FE-SEM), atomic force microscopy and X-ray diffraction. Our results showed that Medusomyces gisevii had higher BC yields in media with sugar concentrations close to 10 g L−1 after a 18–20 days incubation period. Glucose in general lead to a higher BC yield (173 g L−1) compared to sucrose (163.5 g L−1). The BC crystallinity degree and surface roughness were higher in the samples synthetized from sucrose. Obtained FE-SEM micrographs show that the BC pellicles synthesized in the sucrose media contained densely packed tangles of cellulose fibrils whereas the BC produced in the glucose media displayed rather linear geometry of the BC fibrils without noticeable aggregates.
Background
Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing the environmentally harmful Schotten-Baumann reaction. Long-chain acyl-amino acids can serve as strong surfactants and emulsifiers, with application in cosmetic industries. Heterologous expression of these enzymes, however, is often hampered, limiting their use in industrial processes.
Results
We identified a novel mycobacterial aminoacylase gene from Mycolicibacterium smegmatis MKD 8, cloned and expressed it in Escherichia coli and Vibrio natriegens using the T7 overexpression system. The recombinant enzyme was prone to aggregate as inclusion bodies, and while V. natriegens Vmax™ could produce soluble aminoacylase upon induction with isopropyl β-d-1-thiogalactopyranoside (IPTG), E. coli BL21 (DE3) needed autoinduction with lactose to produce soluble recombinant protein. We successfully conducted a chaperone co-expression study in both organisms to further enhance aminoacylase production and found that overexpression of chaperones GroEL/S enhanced aminoacylase activity in the cell-free extract 1.8-fold in V. natriegens and E. coli. Eventually, E. coli ArcticExpress™ (DE3), which co-expresses cold-adapted chaperonins Cpn60/10 from Oleispira antarctica, cultivated at 12 °C, rendered the most suitable expression system for this aminoacylase and exhibited twice the aminoacylase activity in the cell-free extract compared to E. coli BL21 (DE3) with GroEL/S co-expression at 20 °C. The purified aminoacylase was characterized based on hydrolytic activities, being most stable and active at pH 7.0, with a maximum activity at 70 °C, and stability at 40 °C and pH 7.0 for 5 days. The aminoacylase strongly prefers short-chain acyl-amino acids with smaller, hydrophobic amino acid residues. Several long-chain amino acids were fairly accepted in hydrolysis as well, especially N-lauroyl-L-methionine. To initially evaluate the relevance of this aminoacylase for the synthesis of N-acyl-amino acids, we demonstrated that lauroyl-methionine can be synthesized from lauric acid and methionine in an aqueous system.
Conclusion
Our results suggest that the recombinant enzyme is well suited for synthesis reactions and will thus be further investigated.
Even the shortest flight through unknown, cluttered environments requires reliable local path planning algorithms to avoid unforeseen obstacles. The algorithm must evaluate alternative flight paths and identify the best path if an obstacle blocks its way. Commonly, weighted sums are used here. This work shows that weighted Chebyshev distances and factorial achievement scalarising functions are suitable alternatives to weighted sums if combined with the 3DVFH* local path planning algorithm. Both methods considerably reduce the failure probability of simulated flights in various environments. The standard 3DVFH* uses a weighted sum and has a failure probability of 50% in the test environments. A factorial achievement scalarising function, which minimises the worst combination of two out of four objective functions, reaches a failure probability of 26%; A weighted Chebyshev distance, which optimises the worst objective, has a failure probability of 30%. These results show promise for further enhancements and to support broader applicability.
Subtilisins from microbial sources, especially from the Bacillaceae family, are of particular interest for biotechnological applications and serve the currently growing enzyme market as efficient and novel biocatalysts. Biotechnological applications include use in detergents, cosmetics, leather processing, wastewater treatment and pharmaceuticals. To identify a possible candidate for the enzyme market, here we cloned the gene of the subtilisin SPFA from Fictibacillus arsenicus DSM 15822ᵀ (obtained through a data mining-based search) and expressed it in Bacillus subtilis DB104. After production and purification, the protease showed a molecular mass of 27.57 kDa and a pI of 5.8. SPFA displayed hydrolytic activity at a temperature optimum of 80 °C and a very broad pH optimum between 8.5 and 11.5, with high activity up to pH 12.5. SPFA displayed no NaCl dependence but a high NaCl tolerance, with decreasing activity up to concentrations of 5 m NaCl. The stability enhanced with increasing NaCl concentration. Based on its substrate preference for 10 synthetic peptide 4-nitroanilide substrates with three or four amino acids and its phylogenetic classification, SPFA can be assigned to the subgroup of true subtilisins. Moreover, SPFA exhibited high tolerance to 5% (w/v) SDS and 5% H₂O₂ (v/v). The biochemical properties of SPFA, especially its tolerance of remarkably high pH, SDS and H₂O₂, suggest it has potential for biotechnological applications.
The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0–12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications.
Ambitious climate targets affect the competitiveness of industries in the international market. To prevent such industries from moving to other countries in the wake of increased climate protection efforts, cost adjustments may become necessary. Their design requires knowledge of country-specific production costs. Here, we present country-specific cost figures for different production routes of steel, paying particular attention to transportation costs. The data can be used in floor price models aiming to assess the competitiveness of different steel production routes in different countries (Rübbelke, 2022).
The connective tissues such as tendons contain an extracellular matrix (ECM) comprising collagen fibrils scattered within the ground substance. These fibrils are instrumental in lending mechanical stability to tissues. Unfortunately, our understanding of how collagen fibrils reinforce the ECM remains limited, with no direct experimental evidence substantiating current theories. Earlier theoretical studies on collagen fibril reinforcement in the ECM have relied predominantly on the assumption of uniform cylindrical fibers, which is inadequate for modelling collagen fibrils, which possessed tapered ends. Recently, Topçu and colleagues published a paper in the International Journal of Solids and Structures, presenting a generalized shear-lag theory for the transfer of elastic stress between the matrix and fibers with tapered ends. This paper is a positive step towards comprehending the mechanics of the ECM and makes a valuable contribution to formulating a complete theory of collagen fibril reinforcement in the ECM.
Deammonification for nitrogen removal in municipal wastewater in temperate and cold climate zones is currently limited to the side stream of municipal wastewater treatment plants (MWWTP). This study developed a conceptual model of a mainstream deammonification plant, designed for 30,000 P.E., considering possible solutions corresponding to the challenging mainstream conditions in Germany. In addition, the energy-saving potential, nitrogen elimination performance and construction-related costs of mainstream deammonification were compared to a conventional plant model, having a single-stage activated sludge process with upstream denitrification. The results revealed that an additional treatment step by combining chemical precipitation and ultra-fine screening is advantageous prior the mainstream deammonification. Hereby chemical oxygen demand (COD) can be reduced by 80% so that the COD:N ratio can be reduced from 12 to 2.5. Laboratory experiments testing mainstream conditions of temperature (8–20°C), pH (6–9) and COD:N ratio (1–6) showed an achievable volumetric nitrogen removal rate (VNRR) of at least 50 gN/(m3∙d) for various deammonifying sludges from side stream deammonification systems in the state of North Rhine-Westphalia, Germany, where m3 denotes reactor volume. Assuming a retained Norganic content of 0.0035 kgNorg./(P.E.∙d) from the daily loads of N at carbon removal stage and a VNRR of 50 gN/(m3∙d) under mainstream conditions, a resident-specific reactor volume of 0.115 m3/(P.E.) is required for mainstream deammonification. This is in the same order of magnitude as the conventional activated sludge process, i.e., 0.173 m3/(P.E.) for an MWWTP of size class of 4. The conventional plant model yielded a total specific electricity demand of 35 kWh/(P.E.∙a) for the operation of the whole MWWTP and an energy recovery potential of 15.8 kWh/(P.E.∙a) through anaerobic digestion. In contrast, the developed mainstream deammonification model plant would require only a 21.5 kWh/(P.E.∙a) energy demand and result in 24 kWh/(P.E.∙a) energy recovery potential, enabling the mainstream deammonification model plant to be self-sufficient. The retrofitting costs for the implementation of mainstream deammonification in existing conventional MWWTPs are nearly negligible as the existing units like activated sludge reactors, aerators and monitoring technology are reusable. However, the mainstream deammonification must meet the performance requirement of VNRR of about 50 gN/(m3∙d) in this case.
Die kulturgeschichtlichen Stationen des Spielens vom Beginn der Industrialisierung bis zur Jahrtausendwende werden sowohl aus dem Blickwinkel der Designgeschichte als auch aus dem der Game Studies betrachtet. Die Bandbreite der Themen reicht von der Bauhaus-Wurfpuppe bis zur Barbie, vom Japonismus bis zur Pixelgrafik, von der grafischen Raumillusion zu 3D-Umgebungen und Interaktionsgestaltung, vom Brettspiel bis zum Kriegsspielzeug, von den Utopien der beginnenden Neuzeit bis zu merkantilen Absichten digitaler Spielkonzepte.
Das Multimar Wattforum ist das größte Informationszentrum zum Thema Wattenmeer in Deutschland. Auf über 3000 Quadratmetern können große und kleine Besucher:innen eine interaktive Ausstellung über Wale, Watt und Weltnaturerbe bestaunen. Das Wattenmeer ist ein ganz besonderer Lebensraum und bietet vielen Lebewesen Zuflucht. Es wird durch die Gezeiten geprägt, bei Ebbe trocken und der Sonne ausgesetzt, bei Flut nass und salzig. Das neue Erscheinungsbild für das Multimar Wattforum soll die zentralen Werte und Alleinstellungsmerkmale hervorheben und dem Informationszentrum helfen, seiner Aufgabe als bildende, wissenschaftliche und schützende Einrichtung gerecht zu werden. So wurde ein einheitliches und skalierbares Erscheinungsbild und eine flexible Bild- und Wortmarke entwickel
Jedes Jahr werden Hunderttausende Fahrradrahmen produziert. 90 % davon werden in Asien hergestellt und von dort über die ganze Welt versendet. Mehr als 80 % der Rahmen werden nach der Nutzung wieder nach Asien zurückgeschickt und landen dort auf riesigen Fahrradfriedhöfen. Das Kinderfahrradkonzept "KiBix" wirkt diesem Problem entgegen, indem es eine Alternative für das regelmäßige Kaufen von neuen Kinderfahrrädern bietet. Im Fokus stehen der Kinderfahrradrahmen und die Gabel aus Kunststoff, welche im Spritzgussverfahren produziert werden. Das City-Bike ist in ein Abo-Sharing-System mit Rädern in unterschiedlichen Größen eingebettet, welches durch eine monatliche Gebühr wie das Eigene genutzt werden kann. Der hohlgespritzte Rahmen ermöglicht die Integration kleiner Boxen im Inneren, in denen Kinder ihre persönlichen "Schätze" mitnehmen können. Somit vereint das Konzept die drei Segmente Material/Produktion, Sharing-System und die Mitnahme des "Schatzes". "KiBix" gibt Kindern in Zeiten der Digitalisierung und der globalen Erwärmung die Möglichkeit, selbstbestimmt und nachhaltig die Welt zu erkunden.