Refine
Year of publication
- 2014 (301) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (65)
- Fachbereich Chemie und Biotechnologie (46)
- Fachbereich Elektrotechnik und Informationstechnik (43)
- Fachbereich Wirtschaftswissenschaften (41)
- Fachbereich Energietechnik (40)
- INB - Institut für Nano- und Biotechnologien (35)
- IfB - Institut für Bioengineering (25)
- Fachbereich Maschinenbau und Mechatronik (21)
- Fachbereich Luft- und Raumfahrttechnik (13)
- Fachbereich Bauingenieurwesen (12)
Document Type
- Article (142)
- Conference Proceeding (67)
- Part of a Book (30)
- Book (21)
- Conference: Meeting Abstract (15)
- Report (8)
- Other (6)
- Bachelor Thesis (3)
- Patent (2)
- Part of a Periodical (2)
Keywords
- Antarctic Glaciology (1)
- Bloom Taxonomy (1)
- Bone sawing (1)
- COMSOL Multiphysics (1)
- Calorimetric gas sensor (1)
- Cryptographic protocols (1)
- DNA hybridization (1)
- Extraterrestrial Glaciology (1)
- Field-effect sensor (1)
- Glaciological instruments and methods (1)
- Hydrogen peroxide (1)
- LAPS (1)
- Light-addressable Potentiometric Sensor (1)
- LiveLink for MATLAB (1)
- Malicious model (1)
- Multi-sensor system (1)
- Optimization module (1)
- Privacy-enhancing technologies (1)
- Secure multi-party computation (1)
- Subclacial exploration (1)
- Subglacial lakes (1)
- SunRav BookEditor (1)
- Zero-knowledge proofs (1)
- adsorption (1)
- barium strontium titanate (1)
- bioavailability (1)
- carbonized rice husk (1)
- contactless conductivity sensor (1)
- distance learning (1)
- drug metabolising enzymes (1)
- drug–drug interactions (1)
- e-books (1)
- e-issues (1)
- education (1)
- elastomers (1)
- enzymatic (bio)degradation (1)
- field-effect sensor (1)
- high-k material (1)
- human metabolites (1)
- hydrogen peroxide (1)
- impedance spectroscopy (1)
- in-situ monitoring (1)
- lable-free detection (1)
- lipopolysaccharide (1)
- mechanical properties (1)
- multi-functional material (1)
- novel photoexcitation method (1)
- poly(d, l-lactic acid) (1)
- rubber (1)
- softs (1)
- spatial resolution (1)
- supramolecular structures (1)
- surface modification (1)
- theory and modeling (1)
- tilted constant illumination (1)
- training simulator (1)
- transporters (1)
- virtual reality (1)
Persistent infection with the high-risk Human Papillomavirus type 16 (HPV 16) is the causative event for the development of cervical cancer and other malignant tumors of the anogenital tract and of the head and neck. Despite many attempts to develop therapeutic vaccines no candidate has entered late clinical trials. An interesting approach is a DNA based vaccine encompassing the nucleotide sequence of the E6 and E7 viral oncoproteins. Because both proteins are consistently expressed in HPV infected cells they represent excellent targets for immune therapy. Here we report the development of 8 DNA vaccine candidates consisting of differently rearranged HPV-16 E6 and E7 sequences within one molecule providing all naturally occurring epitopes but supposedly lacking transforming activity. The HPV sequences were fused to the J-domain and the SV40 enhancer in order to increase immune responses. We demonstrate that one out of the 8 vaccine candidates induces very strong cellular E6- and E7- specific cellular immune responses in mice and, as shown in regression experiments, efficiently controls growth of HPV 16 positive syngeneic tumors. This data demonstrates the potential of this vaccine candidate to control persistent HPV 16 infection that may lead to malignant disease. It also suggests that different sequence rearrangements influence the immunogenecity by an as yet unknown mechanism.