Refine
Year of publication
Institute
- INB - Institut für Nano- und Biotechnologien (617) (remove)
Language
- English (557)
- German (59)
- Multiple languages (1)
Document Type
- Article (542)
- Conference Proceeding (52)
- Part of a Book (9)
- Doctoral Thesis (4)
- Book (3)
- Patent (3)
- Other (2)
- Report (2)
Keywords
- Biosensor (6)
- Graduiertentagung (4)
- biosensors (4)
- frequency mixing magnetic detection (4)
- LAPS (3)
- Label-free detection (3)
- Light-addressable potentiometric sensor (3)
- capacitive field-effect sensor (3)
- field-effect sensor (3)
- hydrogen peroxide (3)
- magnetic nanoparticles (3)
- tobacco mosaic virus (TMV) (3)
- Acyl-amino acids (2)
- Aminoacylase (2)
- Bacillaceae (2)
- Bacillus atrophaeus (2)
- Biotechnological application (2)
- Calorimetric gas sensor (2)
- Capacitive field-effect sensor (2)
- Hydrogen peroxide (2)
- Raman spectroscopy (2)
- Subtilases (2)
- Subtilisin (2)
- Tobacco mosaic virus (TMV) (2)
- acetoin (2)
- capacitive field-effect sensors (2)
- gold nanoparticles (2)
- light-addressable potentiometric sensor (2)
- microfluidics (2)
- penicillinase (2)
- sterilisation (2)
- (Bio)degradation (1)
- Aachen / Fachhochschule Aachen (1)
- Acylation (1)
- Alginate beads (1)
- Alkalihalobacillus okhensis (1)
- Bacillus atrophaeus spores (1)
- Bio-Sensors (1)
- Bioabsorbable (1)
- Biocatalysis (1)
- Biosensorik (1)
- Biosurfactants (1)
- Broad pH spectrum (1)
- CNOT (1)
- CRISPR/Cas9 (1)
- Capacitive field-effect (1)
- Capacitive model (1)
- Chaperone (1)
- Chaperone co-expression (1)
- Chemical images (1)
- Chemical imaging (1)
- Chemical imaging sensor (1)
- Chemical sensor (1)
- Choleratoxin B (1)
- Coat protein (1)
- C–V method (1)
- DNA biosensor (1)
- DPA (dipicolinic acid) (1)
- Dehydrogenase (1)
- Detergent protease (1)
- Diaphorase (1)
- E. coli detection (1)
- EIS capacitive sensor (1)
- Electrolyte–insulator–semiconductor (1)
- Enzymatic biosensor (1)
- Enzyme coverage (1)
- Enzyme logic gate (1)
- Enzyme nanocarrier (1)
- Extracellular enzymes (1)
- Field effect (1)
- Field-effect biosensor (1)
- Field-effect device (1)
- Field-effect sensor (1)
- GaAs hot electron injector (1)
- Gas sensor (1)
- Glucose biosensor (1)
- Glucose oxidase (1)
- Gold nanoparticles (1)
- Graduate symposium (1)
- Graduierter (1)
- Gunn diode (1)
- Halotolerant protease (1)
- Heavy metal detection (1)
- Hypersecretion (1)
- ISFET (1)
- Impedance spectroscopy (1)
- Inclusion bodies (1)
- Lab-on-Chip (1)
- Layer-by-layer adsorption (1)
- LbL films (1)
- MOS (1)
- Marker-free mutagenesis (1)
- Master stamp (1)
- Multi-sensor system (1)
- Multianalyte detection (1)
- Multicell (1)
- Multiplexing (1)
- Nano Materials (1)
- Nanomaterial (1)
- Nanostructuring (1)
- Nanotechnologie (1)
- Negative impedance convertor (1)
- O2 plasma (1)
- Organic light-emitting diode display (1)
- Penicillin (1)
- Photolithographic mimics (1)
- Plant virus (1)
- Poly(allylamine hydrochloride) (1)
- Poly(d,l-lacticacid) (1)
- Polyimide (1)
- Polylactide acid (1)
- Potentiometry (1)
- Promotionsstudium (1)
- Quartz crystal microbalance (1)
- Real-time monitoring (1)
- Resistive temperature detector (1)
- Resonance-mode measurement (1)
- Silk fibroin (1)
- Simultaneous determination (1)
- Sn₃O₄ (1)
- Stenotrophomonas maltophilia (1)
- Sterilisation process (1)
- Streptomyces griseus (1)
- Streptomyces lividans (1)
- Surface imprinted polymer (1)
- TMV adsorption (1)
- Ta₂O₅ gate (1)
- Tobacco mosaic virus (1)
- Trinkwassersicherheit (1)
- Uracil-phosphoribosyltransferase (1)
- Vibrio natriegens (1)
- Wafer (1)
- XOR (1)
- Zeta potential (1)
- acetoin reductase (1)
- actuator-sensor system (1)
- alcoholic beverages (1)
- aminooctanethiol (1)
- amperometric biosensors (1)
- annealing (1)
- aquaculture (1)
- artificial olfactory image (1)
- aseptic parameters (1)
- aspergillus (1)
- atomic layer deposition (1)
- bi-enzyme biosensor (1)
- bioburdens (1)
- biocompatible (1)
- biocompatible materials (1)
- biodegradabl (1)
- biodegradable electronic devices (1)
- biosensor (1)
- calorimetric gas sensor (1)
- calorimetric gas sensor;hydrogen peroxide;wireless sensor system (1)
- capacitive EIS sensor (1)
- capacitive electrolyte–insulator–semiconductor sensors (1)
- capacitive field-effect biosensor (1)
- capacitive model (1)
- capillary micro-droplet cell (1)
- carbon electrodes (1)
- catalytic decomposition (1)
- catalytic metal (1)
- chemical sensor (1)
- chip-based sensor setup (1)
- cholera toxin B (1)
- colorization (1)
- control gate (1)
- coupled Néel–Brownian relaxation dynamics (1)
- detection of charged macromolecules (1)
- detergent protease (1)
- drinking water safety (1)
- electrical conductivity of liquids (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- electrolyte-insulator-semiconductor capacitors (1)
- electronic nose (1)
- encapsulation materials (1)
- endospores (1)
- enzymatic biosensor (1)
- enzyme cascade (1)
- enzyme immobilization (1)
- enzyme kinetics (1)
- enzyme-logic gate (1)
- equivalent circuit (1)
- fibroin (1)
- field-effect structure (1)
- filamentous fungi (1)
- frequency mixing (1)
- gas sensor (1)
- gaseous hydrogen peroxide (1)
- genome engineering (1)
- glucose (1)
- glucose oxidase (GOx) (1)
- graphene oxide (1)
- halotolerant protease (1)
- heavy metals (1)
- high-alkaline subtilisin (1)
- horseradish peroxidase (HRP) (1)
- hydroxylation (1)
- immobilization (1)
- impedance spectroscopy (1)
- key performance indicators (1)
- layer expansion (1)
- layer-by-layer technique (1)
- light-addressable electrode (1)
- light-addressing technologies (1)
- magnetic actuation (1)
- magnetic beads (1)
- magnetic biosensing (1)
- magnetic frequency mixing technique (1)
- magnetic relaxation (1)
- magnetic sandwich immunoassay (1)
- magnetic sensing (1)
- magnetic sensors (1)
- magnetic separation (1)
- magnetic tweezers (1)
- magnetophoretic velocity (1)
- metal-oxide-semiconductor structure (1)
- micromagnetic simulation (1)
- microwave generation (1)
- multi-sensing platform (1)
- multianalyte detection (1)
- multiparametric immunoassays (1)
- multiplex detection (1)
- nanobelts (1)
- nanomaterials (1)
- nanoparticle coverage (1)
- on-chip integrated addressable EISCAP sensors (1)
- optical sensor setup (1)
- optical spore trapping (1)
- optical trapping (1)
- organic PVC membranes (1)
- organosilanes (1)
- oxidative stable protease (1)
- pH sensors (1)
- pattern-size reduction (1)
- penicillin (1)
- photoelectrochemistry (1)
- plant virus detection (1)
- plug-based microfluidic device (1)
- polyaniline (1)
- polystyrene sulfonate (1)
- recombinant expression (1)
- scanned light pulse technique (1)
- self-aligned patterning (1)
- silanization (1)
- spore kill rate (1)
- sterility (1)
- sterility tests (1)
- sterilization (1)
- sterilization conditions (1)
- sterilization efficacy (1)
- sterilization methods (1)
- superparamagnetic bead (1)
- superparamagnetic nanoparticles (1)
- surface functionalization (1)
- temperature (1)
- thermometry (1)
- thin-film microsensors (1)
- titanium dioxide photoanode (1)
- turnip vein clearing virus (TVCV) (1)
- ultrathin gate insulators (1)
- urease (1)
- validation methods (1)
- visualization (1)
- wafer-level testing (1)
- α-aminoacylase (1)
- ε-lysine acylase (1)
In this study, a high-speed chemical imaging system was developed for visualization of the interior of a microfluidic channel. A microfluidic channel was constructed on the sensor surface of the light-addressable potentiometric sensor (LAPS), on which the ion concentrations could be measured in parallel at up to 64 points illuminated by optical fibers. The temporal change of pH distribution inside the microfluidic channel was recorded at a maximum rate of 100 frames per second (fps). The high frame rate allowed visualization of moving interfaces and plugs in the channel even at a flow velocity of 111 mm/s, which suggests the feasibility of plug-based microfluidic devices for flow-injection analysis (FIA).
Light-addressable potentiometric sensors (LAPS) are field-effect-based sensors. A modulated light source is used to define the particular measurement spot to perform spatially resolved measurements of chemical species and to generate chemical images. In this work, an organic-LED (OLED) display has been chosen as a light source. This allows high measurement resolution and miniaturisation of the system. A new developed driving method for the OLED display optimised for LAPS-based measurements is demonstrated. The new method enables to define modulation frequencies between 1 kHz and 16 kHz and hence, reduces the measurement time of a chemical image by a factor of 40 compared to the traditional addressing of an OLED display.
Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 Å as well as in complex with CoA at a resolution of 1.59 Å. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called “hot dog fold” which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all “hot dog fold” proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041–2053. © 2014 Wiley Periodicals, Inc.
Heavy metal detection with semiconductor devices based on PLD-prepared chalcogenide glass thin films
(2007)
In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.