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Zugriffsart
- campus (2147)
- weltweit (1860)
- bezahl (766)
- fachbereichsweit (FB4) (34)
Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.
Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.
We present new numerical results for shape optimization problems of interior Neumann eigenvalues. This field is not well understood from a theoretical standpoint. The existence of shape maximizers is not proven beyond the first two eigenvalues, so we study the problem numerically. We describe a method to compute the eigenvalues for a given shape that combines the boundary element method with an algorithm for nonlinear eigenvalues. As numerical optimization requires many such evaluations, we put a focus on the efficiency of the method and the implemented routine. The method is well suited for parallelization. Using the resulting fast routines and a specialized parametrization of the shapes, we found improved maxima for several eigenvalues.
Three-dimensional (3D) full-field measurements provide a comprehensive and accurate validation of finite element (FE) models. For the validation, the result of the model and measurements are compared based on two respective point-sets and this requires the point-sets to be registered in one coordinate system. Point-set registration is a non-convex optimization problem that has widely been solved by the ordinary iterative closest point algorithm. However, this approach necessitates a good initialization without which it easily returns a local optimum, i.e. an erroneous registration. The globally optimal iterative closest point (Go-ICP) algorithm has overcome this drawback and forms the basis for the presented open-source tool that can be used for the validation of FE models using 3D full-field measurements. The capability of the tool is demonstrated using an application example from the field of biomechanics. Methodological problems that arise in real-world data and the respective implemented solution approaches are discussed.
Thermohydrodynamic analysis of thrust-bearing with circular pads running on bubbly oil (BTHD-theory)
(1985)
Traditional vulcanization mold manufacturing is complex, costly, and under pressure due to shorter product lifecycles and diverse variations. Additive manufacturing using Fused Filament Fabrication and high-performance polymers like PEEK offer a promising future in this industry. This study assesses the compressive strength of various infill structures (honeycomb, grid, triangle, cubic, and gyroid) when considering two distinct build directions (Z, XY) to enhance PEEK’s economic and resource efficiency in rapid tooling. A comparison with PETG samples shows the behavior of the infill strategies. Additionally, a proof of concept illustrates the application of a PEEK mold in vulcanization. A peak compressive strength of 135.6 MPa was attained in specimens that were 100% solid and subjected to thermal post-treatment. This corresponds to a 20% strength improvement in the Z direction. In terms of time and mechanical properties, the anisotropic grid and isotropic cubic infill have emerged for use in rapid tooling. Furthermore, the study highlights that reducing the layer thickness from 0.15 mm to 0.1 mm can result in a 15% strength increase. The study unveils the successful utilization of a room-temperature FFF-printed PEEK mold in vulcanization injection molding. The parameters and infill strategies identified in this research enable the resource-efficient FFF printing of PEEK without compromising its strength properties. Using PEEK in rapid tooling allows a cost reduction of up to 70% in tool production.
In times of short product life cycles, additive manufacturing and rapid tooling are important methods to make tool development and manufacturing more efficient. High-performance polymers are the key to mold production for prototypes and small series. However, the high temperatures during vulcanization injection molding cause thermal aging and can impair service life. The extent to which the thermal stress over the entire process chain stresses the material and whether it leads to irreversible material aging is evaluated. To this end, a mold made of PEEK is fabricated using fused filament fabrication and examined for its potential application. The mold is heated to 200 ◦C, filled with rubber, and cured. A differential scanning calorimetry analysis of each process step illustrates the crystallization behavior and first indicates the material resistance. It shows distinct cold crystallization regions at a build chamber temperature of 90 ◦C. At an ambient temperature above Tg, crystallization of 30% is achieved, and cold crystallization no longer occurs. Additional tensile tests show a decrease in tensile strength after ten days of thermal aging. The steady decrease in recrystallization temperature indicates degradation of the additives. However, the tensile tests reveal steady embrittlement of the material due to increasing crosslinking.
100 Jahre Design Feier(n) - zwischen Gestern und Morgen; In Gedanken lebt sie weiter - Abriss der ehemaligen Werkkunstschule an der Südstraße; Die Kampfzeit hautnah miterlebt: Rückblick der ehemaligen Dekanin und Rektorin Prof. Dr. Hildegard Reitz; EuRegio-Design-Preis; AachenDesign: ein starker Partner und eine Visitenkarte der Region; "Kegeln": auf der EuroShop 2005; sub-art: gestalterische Faszination einer Naturgewalt; It's Showtime:"Vorhang auf und Filme ab"; "visperanto": Entwicklung einer visuellen Zeichensprache; Harmonie im Zusammenspiel von Kunst und Ausstellungsarchitektur: Ausstellung "Albrecht Dürer - Apelles des Schwarz-Weiß" im Suermondt-Ludwig-Museum; Mamba am Aachener Klinikum; "Gestaltungsoffensive": Designstudierende entwerfen Werbekonzepte für Aachener Online-Tageszeitungen; Ein "Boxhorn" gibt's nicht zweimal; Ziele und Zukunft: "Gestaltung: Kommunikation, Produkt und Medien";
Magazin der Fachhochschule Aachen / Aachen University of Applied Sciences . Nr. 2/2007 Hochschule im Umbruch: FH Aachen am Zug Herausgeber: Rektor der Fachhochschule Aachen Redaktion: Dr. Roger Uhle, Stefanie Erkeling, Sabine Krükel, Daniela Voßenkaul, Cornelia Driesen Übersetzung: Julia Schulze Gestaltung: Prof. Dipl.-Des. Doris Casse-Schlüter, Dipl.-Des. Markus Nailis Dimensionen erfahren 06 Bachelor welcome! 16 Die Bestimmung der Studienbeiträge 19 Auch die Fachhochschule Aachen hat ihre Exzellenz 20 Neue Freiheiten und ein neues Gremium 22 Campus Jülich im Wandel 24 Hochschulmarketing: Mahr als Werbung 25 Auf zu neuen (Service-) Ufern! 26 Der neue Campus Jülich Forschung entwickeln 28 Der Neugierige: Ein Porträt über Prof. Dr. Marcus Baumann ... 30 Wie viel Schadstoff verträgt die Pflanze? 31 "OP der Zukunft" 33 Im Wirkungskreis des Blitzes 34 Schicht für Schicht zum individuellen Produkt 36 Forschen heute für die Herausforderungen von morgen 38 500.000 Euro für "Polymere Materialien" 39 Konstruieren - Simulieren - Produzieren 40 Meldungen: Forschung Studium erleben 42 Auf den Hut gekommen: Jan Lengwenat verhilft seiner Diplomarbeit dem "alten Hut" zu neuem Glanz. 44 Prozesstechnik berufsbegleitend 45 Ein wichtiger Baustein in der Lehre 47 Einblick statt Blackbox 49 Ausstellungen bereichern die Lehre 52 Aufatmen im Triebwerklabor 54 Kreativität und "Kriminalität" 55 Was Studierende studieren, wenn sie gerade nicht studieren... 56 Viel Beifall für die Eigenbau-Humoreske 58 Girls Day mit dem Staatssekretär 61 Messen und Ausstellungen 66 Meldungen: Studium International denken 70 Kompetenzen im Dialog: Prof. Willy Kuhlmann berichtet ... 74 Endlich offiziell: Euregionaler Studiengang C-MD ... 75 Ehrung für besonderes Engagement 76 FH-Wirtschaftsstudierende grüßen aus aller Welt 79 Genau die richtige Mischung 80 Professor, außerordentlich! 81 Der Zeit voraus Personen begegnen 82 Standortfaktor: Wissenschaft 84 Studenten 2.0 86 Wer nicht fragt, ist selber Schuld! 88 Lo(u)s, bergauf! 90 Preise und Auszeichnungen 95 Diplome 2006/2007 98 Personalia Service bieten 100 Vom Auslandsaufenthalt bis zur Jobmesse 102 Das alfha.net in neuem Gewand 105 Der Hochschulsport in Aachen 107 Neuer Blick auf die Didaktik 108 Zeile für Zeile 110 Was ist eigentlich ... ECDL? 111 Impressum