Modification of a computer model of human stem cell-derived cardiomyocyte electrophysiology based on Patch-Clamp measurements
- Human induced pluripotent stem cells (hiPSCs) have shown to be promising in disease studies and drug screenings [1]. Cardiomyocytes derived from hiPSCs have been extensively investigated using patch-clamping and optical methods to compare their electromechanical behaviour relative to fully matured adult cells. Mathematical models can be used for translating findings on hiPSCCMs to adult cells [2] or to better understand the mechanisms of various ion channels when a drug is applied [3,4]. Paci et al. (2013) [3] developed the first model of hiPSC-CMs, which they later refined based on new data [3]. The model is based on iCells® (Fujifilm Cellular Dynamics, Inc. (FCDI), Madison WI, USA) but major differences among several cell lines and even within a single cell line have been found and motivate an approach for creating sample-specific models. We have developed an optimisation algorithm that parameterises the conductances (in S/F=Siemens/Farad) of the latest Paci et al. model (2018) [5] using current-voltage data obtained in individual patch-clamp experiments derived from an automated patch clamp system (Patchliner, Nanion Technologies GmbH, Munich).
Author: | Aravind Hariharan RamanORCiD, Alexander JungORCiD, András Horváth, Nadine Becker, Manfred StaatORCiD |
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DOI: | https://doi.org/10.17185/duepublico/48750 |
ISBN: | 978-3-940402-22-6 |
Parent Title (English): | 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH Aachen |
Publisher: | Universität Duisburg-Essen |
Place of publication: | Duisburg |
Editor: | Manfred StaatORCiD, Daniel Erni |
Document Type: | Conference Proceeding |
Language: | English |
Year of Completion: | 2019 |
Creating Corporation: | MedTech Symposium |
Contributing Corporation: | Young Researchers Academy |
First Page: | 10 |
Last Page: | 11 |
Link: | https://doi.org/10.17185/duepublico/48750 |
Zugriffsart: | weltweit |
Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
FH Aachen / IfB - Institut für Bioengineering | |
open_access (DINI-Set): | open_access |
collections: | Verlag / Universität Duisburg-Essen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |