Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors
- The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.
Author: | Claudia Koch, Arshak PoghossianORCiD, Michael Josef SchöningORCiD, Christian Wege |
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DOI: | https://doi.org/10.7150/ntno.22114 |
ISSN: | 2206-7418 |
Parent Title (English): | Nanotheranostics |
Publisher: | Ivyspring |
Place of publication: | Sydney |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Volume: | 2 |
Issue: | 2 |
First Page: | 184 |
Last Page: | 196 |
Link: | http://doi.org/10.7150/ntno.22114 |
Zugriffsart: | weltweit |
Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
open_access (DINI-Set): | open_access |
collections: | Verlag / Ivyspring |
Open Access / Gold | |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell |