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Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors

  • The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts.

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Metadaten
Author:Claudia Koch, Arshak Poghossian, Michael J. Schöning, Christian Wege
DOI:https://doi.org/10.7150/ntno.22114
ISSN:2206-7418
Parent Title (English):Nanotheranostics
Publisher:Ivyspring
Place of publication:Sydney
Document Type:Article
Language:English
Year of Completion:2018
Date of the Publication (Server):2018/04/30
Volume:2
Issue:2
First Page:184
Last Page:196
Link:http://doi.org/10.7150/ntno.22114
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Open Access
Verlag / Ivyspring
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell