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Field-effect biosensor using virus particles as scaffolds for enzyme immobilization

  • A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples.

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Metadaten
Author:Arshak Poghossian, Melanie Jablonski, Claudia Koch, Thomas Bronder, David Rolka, Christina Wege, Michael J. Schöning
DOI:https://doi.org/10.1016/j.bios.2018.03.036
ISSN:0956-5663
Parent Title (English):Biosensors and Bioelectronics
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of Completion:2018
Date of the Publication (Server):2018/03/27
Volume:110
First Page:168
Last Page:174
Link:http://doi.org/10.1016/j.bios.2018.03.036
Zugriffsart:bezahl
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Elsevier