Thick-film carbon electrode deposited onto a biodegradable fibroin substrate for biosensing applications
- This study addresses a proof-of-concept experiment with a biocompatible screen-printed carbon electrode deposited onto a biocompatible and biodegradable substrate, which is made of fibroin, a protein derived from silk of the Bombyx mori silkworm. To demonstrate the sensor performance, the carbon electrode is functionalized as a glucose biosensor with the enzyme glucose oxidase and encapsulated with a silicone rubber to ensure biocompatibility of the contact wires. The carbon electrode is fabricated by means of thick-film technology including a curing step to solidify the carbon paste. The influence of the curing temperature and curing time on the electrode morphology is analyzed via scanning electron microscopy. The electrochemical characterization of the glucose biosensor is performed by amperometric/voltammetric measurements of different glucose concentrations in phosphate buffer. Herein, systematic studies at applied potentials from 500 to 1200 mV to the carbon working electrode (vs the Ag/AgCl reference electrode) allow to determine the optimal working potential. Additionally, the influence of the curing parameters on the glucose sensitivity is examined over a time period of up to 361 days. The sensor shows a negligible cross-sensitivity toward ascorbic acid, noradrenaline, and adrenaline. The developed biocompatible biosensor is highly promising for future in vivo and epidermal applications.
Verfasserangaben: | Denise MolinnusORCiD, Kevin Alexander JanusORCiD, Anyelina C. Fang, Aleksander Drinic, Stefan AchtsnichtORCiD, Marius Köpf, Michael Keusgen, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.1002/pssa.202200100 |
ISSN: | 1862-6319 |
Titel des übergeordneten Werkes (Englisch): | Physica status solidi (a) |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Erscheinungsjahr: | 2022 |
Datum der Publikation (Server): | 10.10.2022 |
Freies Schlagwort / Tag: | biocompatible materials; biodegradable electronic devices; biosensors; carbon electrodes; glucose |
Jahrgang: | 219 |
Ausgabe / Heft: | 23 |
Erste Seite: | 1 |
Letzte Seite: | 9 |
Bemerkung: | Corresponding author: Michael J. Schöning |
Link: | https://doi.org/10.1002/pssa.202200100 |
Zugriffsart: | weltweit |
Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
open_access (DINI-Set): | open_access |
collections: | Verlag / Wiley-VCH |
Open Access / Hybrid | |
Geförderte OA-Publikationen / DEAL Wiley | |
Lizenz (Deutsch): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |