Spore-based biosensor to monitor the microbicidal efficacy of gaseous hydrogen peroxide sterilization processes
- In this work, a spore-based biosensor is evaluated to monitor the microbicidal efficacy of sterilization processes applying gaseous hydrogen peroxide (H2O2). The sensor is based on interdigitated electrode structures (IDEs) that have been fabricated by means of thin-film technologies. Impedimetric measurements are applied to study the effect of sterilization process on spores of Bacillus atrophaeus. This resilient microorganism is commonly used in industry to proof the sterilization efficiency. The sensor measurements are accompanied by conventional microbiological challenge tests, as well as morphological characterizations with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sensor measurements are correlated with the microbiological test routines. In both methods, namely the sensor-based and microbiological one, a tailing effect has been observed. The results are evaluated and discussed in a three-dimensional calibration plot demonstrating the sensor's suitability to enable a rapid process decision in terms of a successfully performed sterilization.
Author: | Jan OberländerORCiD, Marlena Mayer, Anton Greeff, Michael Keusgen, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.1016/j.bios.2017.12.045 |
ISSN: | 0956-5663 |
Parent Title (English): | Biosensors and Bioelectronics |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Volume: | 104 |
First Page: | 87 |
Last Page: | 94 |
Link: | https://doi.org/10.1016/j.bios.2017.12.045 |
Zugriffsart: | campus |
Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
collections: | Verlag / Elsevier |