An Enzyme-Based Interdigitated Electrode-Type Biosensor for Detecting Low Concentrations of H₂O₂ Vapor/Aerosol
- This work introduces a novel method for the detection of H₂O₂ vapor/aerosol of low concentrations, which is mainly applied in the sterilization of equipment in medical industry. Interdigitated electrode (IDE) structures have been fabricated by means of microfabrication techniques. A differential setup of IDEs was prepared, containing an active sensor element (active IDE) and a passive sensor element (passive IDE), where the former was immobilized with an enzymatic membrane of horseradish peroxidase that is selective towards H₂O₂. Changes in the IDEs’ capacitance values (active sensor element versus passive sensor element) under H₂O₂ vapor/aerosol atmosphere proved the detection in the concentration range up to 630 ppm with a fast response time (<60 s). The influence of relative humidity was also tested with regard to the sensor signal, showing no cross-sensitivity. The repeatability assessment of the IDE biosensors confirmed their stable capacitive signal in eight subsequent cycles of exposure to H₂O₂ vapor/aerosol. Room-temperature detection of H₂O₂ vapor/aerosol with such miniaturized biosensors will allow a future three-dimensional, flexible mapping of aseptic chambers and help to evaluate sterilization assurance in medical industry.
Author: | Farnoosh Vahidpour, Yousef H. M. AlghazaliORCiD, Sevilay Akca, Gregor Hommes, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.3390/chemosensors10060202 |
ISSN: | 2227-9040 |
Parent Title (English): | Chemosensors |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Year of Completion: | 2022 |
Date of first Publication: | 2022/05/26 |
Volume: | 10 |
Issue: | 6 |
Length: | Arikel 202 |
Note: | This article belongs to the Special Issue "Bioinspired Chemical Sensors and Micro-Nano Devices" |
Link: | https://doi.org/10.3390/chemosensors10060202 |
Institutes: | FH Aachen / INB - Institut für Nano- und Biotechnologien |
open_access (DINI-Set): | open_access |
collections: | Verlag / MDPI |
Open Access / Gold | |
Licence (German): | Creative Commons - Namensnennung |