DNA computing system activated by electrochemically triggered DNA realease from a polymer-brush-modified electrode array

  • An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAEMA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at −1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals.

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Author:Maria Gamella, Andrey Zakharchenko, Nataliia Guz, Madeline Masi, Sergiy Minko, Dmitry M. Kolpashchikov, Heiko IkenORCiD, Arshak PoghossianORCiD, Michael Josef SchöningORCiD, Evgeny Katz
DOI:https://doi.org/10.1002/elan.201600389
ISSN:1521-4109
Parent Title (English):Electroanalysis
Publisher:Wiley-VCH
Place of publication:Weinheim
Document Type:Article
Language:English
Year of Completion:2017
Volume:29
Issue:2
First Page:398
Last Page:408
Link:https://doi.org/10.1002/elan.201600389
Zugriffsart:campus
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Wiley-VCH