A semi-quantitative dipstick assay for microcystin

  • An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l−1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.

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Metadaten
Author:Nils TippkötterORCiD, Henning Stückmann, Stephen Kroll, Gunda Winkelmann, Udo Noack, Thomas Scheper, Roland UlberORCiD
DOI:https://doi.org/10.1007/s00216-009-2750-8
ISSN:1618-2650
Parent Title (English):Analytical and Bioanalytical Chemistry
Publisher:springer
Place of publication:Berlin
Document Type:Article
Language:English
Year of Completion:2009
Volume:394
Issue:3
First Page:863
Last Page:869
Link:https://link.springer.com/article/10.1007/s00216-009-2750-8
Zugriffsart:campus
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
collections:Verlag / Springer