Method for production of single-stranded macronucleotides
- The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.
| Author: | Ralf Stadtmüller, Nils TippkötterORCiD, Roland UlberORCiD |
|---|---|
| Document Type: | Patent |
| Language: | English |
| Year of Completion: | 2015 |
| Date of the Publication (Server): | 2020/06/26 |
| Note: | Patent auch unter EP2774996, EP2774996, US2017145460 und US9944966 veröffentlicht. |
| Link: | https://worldwide.espacenet.com/patent/search/family/047877699/publication/US2015252399A1?q=US2015252399 |
| Zugriffsart: | weltweit |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| Patent Number: | US2015252399 |
| Country of Patent Application: | Weltweit |
| Patent Year applied: | 2014 |
| PatentApplication: | 04.03.2014 |
