Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase
- The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
| Author: | Lukas Muschallik, Carina Ronja Kipp, Inga Recker, Johannes BongaertsORCiD, Martina Pohl, Melanie Gelissen, Michael Josef SchöningORCiD, Thorsten Selmer, Petra SiegertORCiD |
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| DOI: | https://doi.org/10.1016/j.jbiotec.2020.09.016 |
| ISBN: | 2590-1559 |
| Parent Title (English): | Journal of Biotechnology |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2020 |
| Date of the Publication (Server): | 2020/09/29 |
| Volume: | 202 |
| Issue: | Vol. 324 |
| First Page: | 61 |
| Last Page: | 70 |
| Link: | https://doi.org/10.1016/j.jbiotec.2020.09.016 |
| Zugriffsart: | campus |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| FH Aachen / Fachbereich Medizintechnik und Technomathematik | |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Elsevier |
