An Enzyme-Based Interdigitated Electrode-Type Biosensor for Detecting Low Concentrations of H₂O₂ Vapor/Aerosol
- This work introduces a novel method for the detection of H₂O₂ vapor/aerosol of low concentrations, which is mainly applied in the sterilization of equipment in medical industry. Interdigitated electrode (IDE) structures have been fabricated by means of microfabrication techniques. A differential setup of IDEs was prepared, containing an active sensor element (active IDE) and a passive sensor element (passive IDE), where the former was immobilized with an enzymatic membrane of horseradish peroxidase that is selective towards H₂O₂. Changes in the IDEs’ capacitance values (active sensor element versus passive sensor element) under H₂O₂ vapor/aerosol atmosphere proved the detection in the concentration range up to 630 ppm with a fast response time (<60 s). The influence of relative humidity was also tested with regard to the sensor signal, showing no cross-sensitivity. The repeatability assessment of the IDE biosensors confirmed their stable capacitive signal in eight subsequent cycles of exposure to H₂O₂ vapor/aerosol. Room-temperature detection of H₂O₂ vapor/aerosol with such miniaturized biosensors will allow a future three-dimensional, flexible mapping of aseptic chambers and help to evaluate sterilization assurance in medical industry.
Verfasserangaben: | Farnoosh Vahidpour, Yousef AlghazaliORCiD, Sevilay Akca, Gregor Hommes, Michael Josef SchöningORCiD |
---|---|
DOI: | https://doi.org/10.3390/chemosensors10060202 |
ISSN: | 2227-9040 |
Titel des übergeordneten Werkes (Englisch): | Chemosensors |
Verlag: | MDPI |
Verlagsort: | Basel |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Erscheinungsjahr: | 2022 |
Datum der Erstveröffentlichung: | 26.05.2022 |
Datum der Publikation (Server): | 25.01.2023 |
Jahrgang: | 10 |
Ausgabe / Heft: | 6 |
Umfang: | Arikel 202 |
Bemerkung: | This article belongs to the Special Issue "Bioinspired Chemical Sensors and Micro-Nano Devices" |
Link: | https://doi.org/10.3390/chemosensors10060202 |
Fachbereiche und Einrichtungen: | FH Aachen / INB - Institut für Nano- und Biotechnologien |
collections: | Verlag / MDPI |
Open Access / Gold | |
Lizenz (Deutsch): | Creative Commons - Namensnennung |