Refine
Year of publication
Document Type
- Article (117) (remove)
Has Fulltext
- no (117) (remove)
Keywords
- damage (2)
- Anastomotic leakage (1)
- Autolysis (1)
- Biocomposites (1)
- Cardiac myocytes (1)
- Cardiac tissue (1)
- CellDrum (1)
- Computational biomechanics (1)
- Constitutive model (1)
- Damage mechanics theory (1)
- Decomposition (1)
- Discontinuous fractures (1)
- Distorsion des oberen Sprunggelenks (1)
- Drug simulation (1)
- ES-FEM (1)
- Electromechanical modeling (1)
- End-to-end colorectal anastomosis (1)
- FS-FEM (1)
- Finite element analysis (1)
- Finite element modelling (1)
Institute
Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.
Load bearing capacity of thin shell structures made of elastoplastic material by direct methods
(2008)
LISA, ein europäisches Projekt zur direkten Berechnung der Tragfähigkeit duktiler Strukturen.
(1998)