Refine
Year of publication
Institute
- IfB - Institut für Bioengineering (121) (remove)
Document Type
- Article (83)
- Conference Proceeding (24)
- Part of a Book (9)
- Book (3)
- Report (2)
Keywords
- Clusterion (5)
- Air purification (4)
- Hämoglobin (4)
- Lipopolysaccharide (4)
- Luftreiniger (4)
- Plasmacluster ion technology (4)
- Raumluft (4)
- Sonde (4)
- Eisschicht (3)
- Kohlenstofffaser (3)
- lipopolysaccharides (3)
- Erythrozyt (2)
- Fibroblast (2)
- Pflanzenphysiologie (2)
- Pflanzenscanner (2)
- Stickstoffmonoxid (2)
- bacterial cellulose (2)
- carbonized rice husk (2)
- celldrum technology (2)
- nanostructured carbonized plant parts (2)
- nanostrukturierte carbonisierte Pflanzenteile (2)
- nitric oxide gas (2)
- plant scanner (2)
- prebiotic (2)
- Adsorption (1)
- Antarctic Glaciology (1)
- Autofluoreszenzverfahren (1)
- Bacillus sp (1)
- Bacterial cellulose (1)
- Bakterien (1)
- Bioreaktor (1)
- Biosolubilization (1)
- Blutzellenlagerung (1)
- Carbon sources (1)
- Cell permeability (1)
- CellDrum (1)
- Cellular force (1)
- Cellulose nanostructure (1)
- Circular Dichroism (1)
- Contractile tension (1)
- Culture media (1)
- Dattel (1)
- Dekontamination (1)
- Dry surfaces (1)
- Elektrodynamik (1)
- Endothelial cells (1)
- Endothelzelle (1)
- Epithel (1)
- Extraterrestrial Glaciology (1)
- FGF23 (1)
- Glaciological instruments and methods (1)
- Harnleiter (1)
- Hemoglobin structure (1)
- Hydrodynamik (1)
- Hydrogel (1)
- Hämoglobinstruktur (1)
- Klotho (1)
- Körpertemperatur (1)
- Lactobacillus rhamnosus GG (1)
- Lichtstreuungsbasierte Instrumente (1)
- Mechanische Beanspruchung (1)
- Medusomyces gisevi (1)
- Microbial adhesion (1)
- NONOate (1)
- Natriumhypochlorit (1)
- Niacin (1)
- Nitric Oxide (1)
- Nitric Oxide Donor (1)
- Organkultur (1)
- PTH (1)
- Pflanzenstress (1)
- Phosphate (1)
- Proteine (1)
- Recombinant activated protein C (1)
- Red blood cell storage (1)
- Riboflavin (1)
- Sampling methods (1)
- Sepsis (1)
- Small Aral Sea (1)
- Subclacial exploration (1)
- Subglacial lakes (1)
- SunRav BookEditor (1)
- Surface microorganisms (1)
- Swabbing (1)
- Thiamine (1)
- Tissue Engineering (1)
- Vitamin A (1)
- Vitamin B (1)
- Vitamin D (1)
- Wasserbrücke (1)
- Wasserstoffperoxid (1)
- Wundheilung (1)
- activated nanostructured carbon (1)
- adsorption (1)
- aktivierte nanostrukturierte Kohlenstofffaser (1)
- autofluorescence-based detection system (1)
- biofilms (1)
- coculture (1)
- contractile tension (1)
- crop yield (1)
- cytosolic water diffusion (1)
- date palm tree (1)
- distance learning (1)
- e-books (1)
- e-issues (1)
- ecological structure (1)
- epithelization (1)
- exopolysaccharides (1)
- hemoglobin (1)
- hemoglobin dynamics (1)
- human dermal fibroblasts (1)
- humic acid (1)
- hydrogel (1)
- immobilization (1)
- kontraktile Spannung (1)
- light scattering analysis (1)
- lignite (1)
- lipopolysaccharide (1)
- low-rank coal (1)
- metagenomics (1)
- microbial diversity (1)
- plant stress (1)
- plasma generated ions (1)
- protein (1)
- pullulan (1)
- rhAPC (1)
- shotgun sequencing (1)
- softs (1)
- soil amendment (1)
- soil health (1)
- soil remediation (1)
- subglacial aquatic ecosystems (1)
- subsurface ice (1)
- subsurface ice research (1)
- subsurface probe (1)
- surface modification (1)
- water bridge phenomenon (1)
- wound healing (1)
There is significant interest in sampling subglacial environments for geobiological studies, but they are difficult to access. Existing ice-drilling technologies make it cumbersome to maintain microbiologically clean access for sample acquisition and environmental stewardship of potentially fragile subglacial aquatic ecosystems. The IceMole is a maneuverable subsurface ice probe for clean in situ analysis and sampling of glacial ice and subglacial materials. The design is based on the novel concept of combining melting and mechanical propulsion. It can change melting direction by differential heating of the melting head and optional side-wall heaters. The first two prototypes were successfully tested between 2010 and 2012 on glaciers in Switzerland and Iceland. They demonstrated downward, horizontal and upward melting, as well as curve driving and dirt layer penetration. A more advanced probe is currently under development as part of the Enceladus Explorer (EnEx) project. It offers systems for obstacle avoidance, target detection, and navigation in ice. For the EnEx-IceMole, we will pay particular attention to clean protocols for the sampling of subglacial materials for biogeochemical analysis. We plan to use this probe for clean access into a unique subglacial aquatic environment at Blood Falls, Antarctica, with return of a subglacial brine sample.
Sampling of dry surfaces for microorganisms is a main component of microbiological safety and is of critical importance in many fields including epidemiology, astrobiology as well as numerous branches of medical and food manufacturing. Aspects of biofilm formation, analysis and removal in aqueous solutions have been thoroughly discussed in literature. In contrast, microbial communities on air-exposed (dry) surfaces have received significantly less attention. Diverse surface sampling methods have been developed in order to address various surfaces and microbial groups, but they notoriously show poor repeatability, low recovery rates and suffer from lack of mutual consistency. Quantitative sampling for viable microorganisms represents a particular challenge, especially on porous and irregular surfaces. Therefore, it is essential to examine in depth the factors involved in microorganisms’ recovery efficiency and accuracy depending on the sampling technique used. Microbial colonization, retention and community composition on different dry surfaces are very complex and rely on numerous physicochemical and biological factors. This study is devoted to analyze and review the (a) physical phenomena and intermolecular forces relevant for microbiological surface sampling; (b) challenges and problems faced by existing sampling methods for viable microorganisms and (c) current directions of engineering and research aimed at improvement of quality and efficiency of microbiological surface sampling.
Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids
(2018)
Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach.
Background
Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min.
Results
Major results were:
1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment,
2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and
3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature.
Conclusion
The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell.
The importance of the availability of stored blood or blood cells, respectively, for urgent transfusion cannot be overestimated. Nowadays, blood storage becomes even more important since blood products are used for epidemiological studies, bio-technical research or banked for transfusion purposes. Thus blood samples must not only be processed, stored, and shipped to preserve their efficacy and safety, but also all parameters of storage must be recorded and reported for Quality Assurance. Therefore, blood banks and clinical research facilities are seeking more accurate, automated means for blood storage and blood processing.
Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate.