Refine
Year of publication
Document Type
- Article (540)
- Conference Proceeding (52)
- Part of a Book (9)
- Doctoral Thesis (4)
- Book (3)
- Other (2)
- Report (2)
- Patent (1)
Language
- English (555)
- German (57)
- Multiple languages (1)
Keywords
- Biosensor (6)
- Graduiertentagung (4)
- biosensors (4)
- frequency mixing magnetic detection (4)
- LAPS (3)
- Label-free detection (3)
- Light-addressable potentiometric sensor (3)
- capacitive field-effect sensor (3)
- field-effect sensor (3)
- hydrogen peroxide (3)
- magnetic nanoparticles (3)
- tobacco mosaic virus (TMV) (3)
- Acyl-amino acids (2)
- Aminoacylase (2)
- Bacillaceae (2)
- Bacillus atrophaeus (2)
- Biotechnological application (2)
- Calorimetric gas sensor (2)
- Capacitive field-effect sensor (2)
- Hydrogen peroxide (2)
- Raman spectroscopy (2)
- Subtilases (2)
- Subtilisin (2)
- Tobacco mosaic virus (TMV) (2)
- acetoin (2)
- capacitive field-effect sensors (2)
- gold nanoparticles (2)
- light-addressable potentiometric sensor (2)
- penicillinase (2)
- sterilisation (2)
- (Bio)degradation (1)
- Aachen / Fachhochschule Aachen (1)
- Acylation (1)
- Alginate beads (1)
- Alkalihalobacillus okhensis (1)
- Bacillus atrophaeus spores (1)
- Bio-Sensors (1)
- Bioabsorbable (1)
- Biocatalysis (1)
- Biosensorik (1)
- Biosurfactants (1)
- Broad pH spectrum (1)
- CNOT (1)
- CRISPR/Cas9 (1)
- Capacitive field-effect (1)
- Capacitive model (1)
- Chaperone (1)
- Chaperone co-expression (1)
- Chemical images (1)
- Chemical imaging (1)
- Chemical imaging sensor (1)
- Chemical sensor (1)
- Choleratoxin B (1)
- Coat protein (1)
- C–V method (1)
- DNA biosensor (1)
- DPA (dipicolinic acid) (1)
- Dehydrogenase (1)
- Detergent protease (1)
- Diaphorase (1)
- EIS capacitive sensor (1)
- Electrolyte–insulator–semiconductor (1)
- Enzymatic biosensor (1)
- Enzyme coverage (1)
- Enzyme logic gate (1)
- Enzyme nanocarrier (1)
- Extracellular enzymes (1)
- Field effect (1)
- Field-effect biosensor (1)
- Field-effect device (1)
- Field-effect sensor (1)
- GaAs hot electron injector (1)
- Gas sensor (1)
- Glucose biosensor (1)
- Glucose oxidase (1)
- Gold nanoparticles (1)
- Graduate symposium (1)
- Graduierter (1)
- Gunn diode (1)
- Halotolerant protease (1)
- Heavy metal detection (1)
- Hypersecretion (1)
- ISFET (1)
- Impedance spectroscopy (1)
- Inclusion bodies (1)
- Lab-on-Chip (1)
- Layer-by-layer adsorption (1)
- LbL films (1)
- MOS (1)
- Marker-free mutagenesis (1)
- Multi-sensor system (1)
- Multianalyte detection (1)
- Multicell (1)
- Multiplexing (1)
- Nano Materials (1)
- Nanomaterial (1)
- Nanostructuring (1)
- Nanotechnologie (1)
- Negative impedance convertor (1)
- O2 plasma (1)
- Organic light-emitting diode display (1)
- Penicillin (1)
- Plant virus (1)
- Poly(allylamine hydrochloride) (1)
- Poly(d,l-lacticacid) (1)
- Polyimide (1)
- Polylactide acid (1)
- Potentiometry (1)
- Promotionsstudium (1)
- Real-time monitoring (1)
- Resistive temperature detector (1)
- Resonance-mode measurement (1)
- Silk fibroin (1)
- Simultaneous determination (1)
- Sn₃O₄ (1)
- Stenotrophomonas maltophilia (1)
- Sterilisation process (1)
- Streptomyces griseus (1)
- Streptomyces lividans (1)
- TMV adsorption (1)
- Ta₂O₅ gate (1)
- Tobacco mosaic virus (1)
- Trinkwassersicherheit (1)
- Uracil-phosphoribosyltransferase (1)
- Vibrio natriegens (1)
- Wafer (1)
- XOR (1)
- Zeta potential (1)
- acetoin reductase (1)
- actuator-sensor system (1)
- alcoholic beverages (1)
- aminooctanethiol (1)
- amperometric biosensors (1)
- annealing (1)
- artificial olfactory image (1)
- aseptic parameters (1)
- aspergillus (1)
- atomic layer deposition (1)
- bi-enzyme biosensor (1)
- bioburdens (1)
- biocompatible (1)
- biocompatible materials (1)
- biodegradabl (1)
- biodegradable electronic devices (1)
- biosensor (1)
- calorimetric gas sensor (1)
- calorimetric gas sensor;hydrogen peroxide;wireless sensor system (1)
- capacitive EIS sensor (1)
- capacitive electrolyte–insulator–semiconductor sensors (1)
- capacitive field-effect biosensor (1)
- capacitive model (1)
- capillary micro-droplet cell (1)
- carbon electrodes (1)
- catalytic decomposition (1)
- catalytic metal (1)
- chemical sensor (1)
- cholera toxin B (1)
- colorization (1)
- control gate (1)
- coupled Néel–Brownian relaxation dynamics (1)
- detection of charged macromolecules (1)
- detergent protease (1)
- drinking water safety (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- electrolyte-insulator-semiconductor capacitors (1)
- electronic nose (1)
- encapsulation materials (1)
- endospores (1)
- enzymatic biosensor (1)
- enzyme cascade (1)
- enzyme immobilization (1)
- enzyme kinetics (1)
- enzyme-logic gate (1)
- equivalent circuit (1)
- fibroin (1)
- field-effect structure (1)
- filamentous fungi (1)
- frequency mixing (1)
- gas sensor (1)
- gaseous hydrogen peroxide (1)
- genome engineering (1)
- glucose (1)
- glucose oxidase (GOx) (1)
- graphene oxide (1)
- halotolerant protease (1)
- heavy metals (1)
- high-alkaline subtilisin (1)
- horseradish peroxidase (HRP) (1)
- hydroxylation (1)
- immobilization (1)
- key performance indicators (1)
- layer expansion (1)
- layer-by-layer technique (1)
- light-addressable electrode (1)
- light-addressing technologies (1)
- magnetic actuation (1)
- magnetic beads (1)
- magnetic biosensing (1)
- magnetic frequency mixing technique (1)
- magnetic relaxation (1)
- magnetic sandwich immunoassay (1)
- magnetic sensing (1)
- magnetic sensors (1)
- magnetic separation (1)
- magnetic tweezers (1)
- magnetophoretic velocity (1)
- metal-oxide-semiconductor structure (1)
- microfluidics (1)
- micromagnetic simulation (1)
- microwave generation (1)
- multi-sensing platform (1)
- multianalyte detection (1)
- multiparametric immunoassays (1)
- multiplex detection (1)
- nanobelts (1)
- nanomaterials (1)
- nanoparticle coverage (1)
- on-chip integrated addressable EISCAP sensors (1)
- optical sensor setup (1)
- optical spore trapping (1)
- optical trapping (1)
- organic PVC membranes (1)
- organosilanes (1)
- oxidative stable protease (1)
- pH sensors (1)
- pattern-size reduction (1)
- penicillin (1)
- photoelectrochemistry (1)
- plant virus detection (1)
- plug-based microfluidic device (1)
- polyaniline (1)
- polystyrene sulfonate (1)
- recombinant expression (1)
- scanned light pulse technique (1)
- self-aligned patterning (1)
- silanization (1)
- spore kill rate (1)
- sterility (1)
- sterility tests (1)
- sterilization (1)
- sterilization conditions (1)
- sterilization efficacy (1)
- sterilization methods (1)
- superparamagnetic bead (1)
- superparamagnetic nanoparticles (1)
- surface functionalization (1)
- temperature (1)
- thin-film microsensors (1)
- titanium dioxide photoanode (1)
- turnip vein clearing virus (TVCV) (1)
- ultrathin gate insulators (1)
- urease (1)
- validation methods (1)
- visualization (1)
- wafer-level testing (1)
- α-aminoacylase (1)
- ε-lysine acylase (1)
Institute
- INB - Institut für Nano- und Biotechnologien (613) (remove)
An array of electrically isolated nanoplate field-effect silicon-on-insulator (SOI) capacitors as a new transducer structure for multiparameter (bio-)chemical sensing is presented. The proposed approach allows addressable biasing and electrical readout of multiple nanoplate field-effect capacitive (bio-)chemical sensors on the same SOI chip, as well as differential-mode measurements. The realized sensor chip has been applied for pH and penicillin concentration measurements, electrical monitoring of polyelectrolyte multilayer formation, and the label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization and denaturation events.
A novel photoexcitation method for the light-addressable potentiometric sensor (LAPS) is proposed to achieve a higher spatial resolution of chemical images. The proposed method employs a combined light source that consists of a modulated light probe, which generates the alternating photocurrent signal, and a ring of constant illumination surrounding it. The constant illumination generates a sheath of carriers with increased concentration which suppresses the spread of photocarriers by enhanced recombination. A device simulation was carried out to verify the effect of constant illumination on the spatial resolution, which demonstrated that a higher spatial resolution can be obtained.
"Biologie trifft Mikroelektronik", das Motto des Instituts für Nano- und Biotechnologien (INB) an der FH Aachen, unterstreicht die zunehmende Bedeutung interdisziplinär geprägter Forschungsaktivitäten. Der thematische Zusammenschluss grundständiger Disziplinen, wie die Physik, Elektrotechnik, Chemie, Biologie sowie die Materialwissenschaften, lässt neue Forschungsgebiete entstehen, ein herausragendes Beispiel hierfür ist die Nanotechnologie: Hier werden neue Werkstoffe und Materialien entwickelt, einzelne Nanopartikel oder Moleküle und deren Wechselwirkung untersucht oder Schichtstrukturen im Nanometerbereich aufgebaut, die neue und vorher nicht bekannte Eigenschaften hervorbringen.
Vor diesem Hintergrund bündelt das im Jahre 2006 gegründete INB die an der FH Aachen vorhandenen Kompetenzen von derzeit insgesamt sieben Laboratorien auf den Gebieten der Halbleitertechnik und Nanoelektronik, Nanostrukturen und DNA-Sensorik, der Chemo- und Biosensorik, der Enzymtechnologie, der Mikrobiologie und Pflanzenbiotechnologie, der Zellkulturtechnik, sowie der Roten Biotechnologie synergetisch. In der Nano- und Biotechnologie steckt außergewöhnliches Potenzial! Nicht zuletzt deshalb stellen sich die Forscher der Herausforderung, in diesem Bereich gemeinsam zu forschen und Schnittstellen zu nutzen, um so bei der Gestaltung neuartiger Ideen und Produkte mitzuwirken, die zukünftig unser alltägliches Leben verändern werden.
Im Folgenden werden die verschiedenen Forschungsbereiche kurz zusammenfassend vorgestellt und vorhandene Interaktionen anhand von exemplarisch ausgewählten, aktuellen Forschungsprojekten skizziert.
Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer
(2014)
Among the variety of transducer concepts proposed for label-free detection of biomolecules, the semiconductor field-effect device (FED) is one of the most attractive platforms. As medical techniques continue to progress towards diagnostic and therapies based on biomarkers, the ability of FEDs for a label-free, fast and real-time detection of multiple pathogenic and physiologically relevant molecules with high specificity and sensitivity offers very promising prospects for their application in point-of-care and personalized medicine for an early diagnosis and treatment of diseases. The presented paper reviews recent advances and current trends in research and development of different FEDs for label-free, direct electrical detection of charged biomolecules by their intrinsic molecular charge. The authors are mainly focusing on the detection of the DNA hybridization event, antibody-antigen affinity reaction as well as clinically relevant biomolecules such as cardiac and cancer biomarkers.
A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al–p-Si–SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent–voltage (I–V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I–V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge.
The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.
The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.
Planar and three-dimensional (3D) interdigitated electrodes (IDE) with electrode digits separated by an insulating barrier of different heights were electrochemically characterized and compared in terms of their sensing properties. Due to the impact of the surface resistance, both types of IDE structures display a non-linear behavior in low-ionic strength solutions. The experimental data were fitted to an electrical equivalent circuit and interpreted taking into account the surface-charge-governed properties. The effect of a charged polyelectrolyte layer electrostatically assembled onto the sensor surface on the surface resistance in solutions with different KCl concentration is studied. In case of the same electrode footprint, 3D-IDEs show a larger cell constant and a higher sensitivity to molecular adsorption than that of planar IDEs. The obtained results demonstrate the potential of 3D-IDEs as a new transducer structure for a direct label-free sensing of charged molecules.