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Meiotic functions of RAD18
(2011)
Elastomers are exceptional materials owing to their ability to undergo large deformations before failure. However, due to their very low stiffness, they are not always suitable for industrial applications. Addition of filler particles provides reinforcing effects and thus enhances the material properties that render them more versatile for applications like tyres etc. However, deformation behavior of filled polymers is accompanied by several nonlinear effects like Mullins and Payne effect. To this day, the physical and chemical changes resulting in such nonlinear effect remain an active area of research. In this work, we develop a heterogeneous (or multiphase) constitutive model at the mesoscale explicitly considering filler particle aggregates, elastomeric matrix and their mechanical interaction through an approximate interface layer. The developed constitutive model is used to demonstrate cluster breakage, also, as one of the possible sources for Mullins effect observed in non-crystallizing filled elastomers.
Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.
It is well known that the already large dielectric constants of some electrolytes like BaTiO₃ can be enhanced further by adding metallic (e.g. Ni, Cu or Ag) nanoparticles. The enhancement can be quite large, a factor of more than 1000 is possible. The consequences for the properties will be discussed in the present paper applying a brick-layer model (BLM) for calculating dc-resistivities of thin layers and a modified one (PBLM) that includes percolation for calculating dielectric properties of these materials. The PBLM results in an at least qualitative description and understanding of the physical phenomena: This model gives an explanation for the steep increase of the dielectric constant below the percolation threshold and why this increase is connected to a dramatic decrease of the breakdown voltage as well as the ability of storing electrical energy. We conclude that metallic electrolyte composites like BaTiO₃ are not appropriate for energy storage.
The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.
Seismic behavior of an existing unreinforced masonry building built pre-modern code, located in the City of Ohrid, Republic of North Macedonia has been investigated in this paper. The analyzed school building is selected as an archetype in an ongoing project named “Seismic vulnerability assessment of existing masonry structures in Republic of North Macedonia (SeismoWall)”. Two independent segments were included in this research: Seismic hazard assessment by creating a cite specific response spectra and Seismic vulnerability definition by creating a region - specific series of vulnerability curves for the chosen building topology. A reliable Seismic Hazard Assessment for a selected region is a crucial point for performing a seismic risk analysis of a characteristic building class. In that manner, a scenario – based method that incorporates together the knowledge of tectonic style of the considered region, the active fault characterization, the earth crust model and the historical seismicity named Neo Deterministic approach is used for calculation of the response spectra for the location of the building. Variations of the rupturing process are taken into account in the nucleation point of the rupture, in the rupture velocity pattern and in the istribution of the slip on the fault. The results obtained from the multiple scenarios are obtained as an envelope of the response spectra computed for the cite using the procedure Maximum Credible Seismic Input (MCSI). Capacity of the selected building has been determined by using nonlinear static analysis. MINEA software (SDA Engineering) was used for verification of the structural safety of the chosen unreinforced masonry structure. In the process of optimization of the number of samples, computational cost required in a Monte Carlo simulation is significantly reduced since the simulation is performed on a polynomial response surface function for prediction of the structural response. Performance point, found as the intersection of the capacity of the building and the spectra used, is chosen as a response parameter. Five levels of damage limit states based on the capacity curve of the building are defined in dependency on the yield displacement and the maximum displacement. Maximum likelihood estimation procedure is utilized in the process of vulnerability curves determination. As a result, region specific series of vulnerability curves for the chosen type of masonry structures are defined. The obtained probabilities of exceedance a specific damage states as a result from vulnerability curves are compared with the observed damages happened after the earthquake in July 2017 in the City of Ohrid, North Macedonia.