Visualization of the recovery process of defects in a cultured cell layer by chemical imaging sensor
- The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) in the sample. In this study, a novel cell assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the recovery of defects in a cell layer brought into proximity of the sensing surface. A reduced impedance at a defect formed artificially in a cell layer was successfully visualized in a photocurrent image. The cell layer was cultured over two weeks, during which the temporal change of the photocurrent distribution corresponding to the recovery of the defect was observed.
| Verfasserangaben: | Ko-ichiro Miyamoto, Bing Yu, Hiroko Isoda, Torsten WagnerORCiD, Michael Josef SchöningORCiD, Tatsuo Yoshinobu |
|---|---|
| DOI: | https://doi.org/10.1016/j.snb.2016.04.018 |
| ISSN: | 0925-4005 |
| Titel des übergeordneten Werkes (Deutsch): | Sensors and Actuators B: Chemical |
| Verlag: | Elsevier |
| Verlagsort: | Amsterdam |
| Dokumentart: | Wissenschaftlicher Artikel |
| Sprache: | Deutsch |
| Erscheinungsjahr: | 2016 |
| Datum der Publikation (Server): | 18.04.2016 |
| Jahrgang: | 236 |
| Erste Seite: | 965 |
| Letzte Seite: | 969 |
| Link: | https://doi.org/10.1016/j.snb.2016.04.018 |
| Zugriffsart: | bezahl |
| Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Elsevier |
