- Treffer 1 von 1
Capacitive model of enzyme-modified field-effect biosensors: Impact of enzyme coverage
- Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.
Verfasserangaben: | Tobias KarschuckORCiD, Arshak PoghossianORCiD, Joey Ser, Astghik Tsokolakyan, Stefan AchtsnichtORCiD, Patrick Wagner, Michael Josef SchöningORCiD |
---|---|
DOI: | https://doi.org/10.1016/j.snb.2024.135530 |
ISSN: | 0925-4005 (Print) |
ISSN: | 1873-3077 (Online) |
Titel des übergeordneten Werkes (Englisch): | Sensors and Actuators B: Chemical |
Verlag: | Elsevier |
Verlagsort: | Amsterdam |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Erscheinungsjahr: | 2024 |
Datum der Publikation (Server): | 11.04.2024 |
Freies Schlagwort / Tag: | Capacitive model; Enzyme coverage; Field-effect biosensor; Multianalyte detection; Penicillin |
Jahrgang: | 408 |
Umfang: | 12 Seiten |
Bemerkung: | Corresponding Author: Michael J. Schöning |
Link: | https://doi.org/10.1016/j.snb.2024.135530 |
Zugriffsart: | weltweit |
Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
open_access (DINI-Set): | open_access |
collections: | Open Access / Hybrid |
Geförderte OA-Publikationen / DEAL Elsevier | |
Lizenz (Deutsch): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |