Application of a portable multi-analyte biosensor for organic acid determination in silage
- Multi-analyte biosensors may offer the opportunity to perform cost-effective and rapid analysis with reduced sample volume, as compared to electrochemical biosensing of each analyte individually. This work describes the development of an enzyme-based biosensor system for multi-parametric determination of four different organic acids. The biosensor array comprises five working electrodes for simultaneous sensing of ethanol, formate, d-lactate, and l-lactate, and an integrated counter electrode. Storage stability of the biosensor was evaluated under different conditions (stored at +4 °C in buffer solution and dry at −21 °C, +4 °C, and room temperature) over a period of 140 days. After repeated and regular application, the individual sensing electrodes exhibited the best stability when stored at −21 °C. Furthermore, measurements in silage samples (maize and sugarcane silage) were conducted with the portable biosensor system. Comparison with a conventional photometric technique demonstrated successful employment for rapid monitoring of complex media.
Author: | Johanna Pilas, Y. Yazici, Thorsten Selmer, M. Keusgen, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.3390/s18051470 |
ISSN: | 1424-8220 |
Parent Title (English): | Sensors |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Year of Completion: | 2018 |
Volume: | 18 |
Issue: | 5 |
Length: | 12 Seiten |
Link: | https://doi.org/10.3390/s18051470 |
Zugriffsart: | weltweit |
Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
FH Aachen / Fachbereich Medizintechnik und Technomathematik | |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
collections: | Verlag / MDPI |
Open Access / Gold |