Development of a multi‐parameter sensor chip for the simultaneous detection of organic compounds in biogas processes
- An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.
Author: | Johanna Pilas, Heiko IkenORCiD, Thorsten Selmer, Michael Keusgen, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.1002/pssa.201431894 |
ISSN: | 1862-6319 |
Parent Title (English): | Physica status solidi (a) |
Publisher: | Wiley |
Place of publication: | Weinheim |
Document Type: | Article |
Language: | English |
Year of Completion: | 2015 |
Volume: | 212 |
Issue: | 6 |
First Page: | 1306 |
Last Page: | 1312 |
Link: | https://doi.org/10.1002/pssa.201431894 |
Zugriffsart: | campus |
Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
FH Aachen / Fachbereich Medizintechnik und Technomathematik | |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
collections: | Verlag / Wiley |