Functionalized magnetizable particles for downstream processing in single-use systems

  • Biotechnological downstream processing is usually an elaborate procedure, requiring a multitude of unit operations to isolate the target component. Besides the disadvantageous space-time yield, the risks of cross-contaminations and product loss grow fast with the complexity of the isolation procedure. A significant reduction of unit operations can be achieved by application of magnetic particles, especially if these are functionalized with affinity ligands. As magnetic susceptible materials are highly uncommon in biotechnological processes, target binding and selective separation of such particles from fermentation or reactions broths can be done in a single step. Since the magnetizable particles can be produced from iron salts and low priced polymers, a single-use implementation of these systems is highly conceivable. In this article, the principles of magnetizable particles, their synthesis and functionalization are explained. Furthermore, applications in the area of reaction engineering, microfluidics and downstream processing are discussed focusing on established single-use technologies and development potential.

Export metadata

Additional Services

Share in X Search Google Scholar
Metadaten
Author:Nils TippkötterORCiD, Huschyar Al-Kaidy, Steffen Wollny, Roland UlberORCiD
DOI:https://doi.org/10.1002/cite.201200130
Parent Title (English):Chemie Ingenieur Technik
Publisher:Wiley
Place of publication:Weinheim
Document Type:Article
Language:English
Year of Completion:2013
Volume:85
Issue:1-2: Special Issue: Single-Use Technology
First Page:76
Last Page:86
Link:http://onlinelibrary.wiley.com/doi/10.1002/cite.201200130/epdf
Zugriffsart:campus
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
collections:Verlag / Wiley