Article
Refine
Year of publication
Document Type
- Article (56) (remove)
Keywords
- CellDrum (3)
- Lipopolysaccharide (2)
- Bioreaktor (1)
- Blutzellenlagerung (1)
- Cardiac myocytes (1)
- Cardiac tissue (1)
- Cell permeability (1)
- Cellular force (1)
- Circular Dichroism (1)
- Computational biomechanics (1)
- Contractile tension (1)
- Drug simulation (1)
- Electromechanical modeling (1)
- Endothelial cells (1)
- Epithel (1)
- Fibroblast (1)
- Frequency adaption (1)
- Harnleiter (1)
- Heart tissue culture (1)
- Hemoglobin structure (1)
- Hodgkin–Huxley models (1)
- Homogenization (1)
- Hämoglobin (1)
- Hämoglobinstruktur (1)
- Induced pluripotent stem cells (1)
- Inotropic compounds (1)
- Ion channels (1)
- Kohlenstofffaser (1)
- LPS (1)
- NONOate (1)
- Nitric Oxide (1)
- Nitric Oxide Donor (1)
- Organkultur (1)
- Pflanzenphysiologie (1)
- Pflanzenscanner (1)
- Pflanzenstress (1)
- Pharmacology (1)
- Recombinant activated protein C (1)
- Red blood cell storage (1)
- Septic cardiomyopathy (1)
- Tissue Engineering (1)
- Wundheilung (1)
- actin cytoskeleton (1)
- carbonized rice husk (1)
- cardiomyocyte biomechanics (1)
- epithelization (1)
- hiPS cardiomyocytes (1)
- human dermal fibroblasts (1)
- lipopolysaccharides (1)
- nanostructured carbonized plant parts (1)
- nanostrukturierte carbonisierte Pflanzenteile (1)
- plant scanner (1)
- plant stress (1)
- wound healing (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (56) (remove)
Red blood cell aggregation in experimental sepsis . Baskurt, O. K.; Temiz, A.; Meiselman, H. J.
(1997)
The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days.
Plant physiology and plant stress: Plant physiology will be much more important for human mankind because of yield and cultivation limits of crops determined by their resistance to stress. To assess and counteract various stress factors it is necessary to conduct plant research to gain information and results on plant physiology.
Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes
(2013)
The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.