Refine
Year of publication
Document Type
- Article (59)
- Conference Proceeding (13)
- Part of a Book (4)
- Book (1)
Language
- English (77) (remove)
Keywords
- Clusterion (4)
- Lipopolysaccharide (4)
- Air purification (3)
- CellDrum (3)
- Kohlenstofffaser (3)
- Luftreiniger (3)
- Plasmacluster ion technology (3)
- Raumluft (3)
- lipopolysaccharides (3)
- Fibroblast (2)
- Hämoglobin (2)
- Pflanzenphysiologie (2)
- Pflanzenscanner (2)
- celldrum technology (2)
- nanostructured carbonized plant parts (2)
- nanostrukturierte carbonisierte Pflanzenteile (2)
- plant scanner (2)
- Adsorption (1)
- Bakterien (1)
- Bioreaktor (1)
- Bladder (1)
- Blutzellenlagerung (1)
- Cardiac myocytes (1)
- Cardiac tissue (1)
- Cell permeability (1)
- Cellular force (1)
- Circular Dichroism (1)
- Computational biomechanics (1)
- Contractile tension (1)
- Dattel (1)
- Dekontamination (1)
- Drug simulation (1)
- Electromechanical modeling (1)
- Endothelial cells (1)
- Endothelzelle (1)
- Epithel (1)
- Erythrozyt (1)
- Frequency adaption (1)
- Growth modelling (1)
- Harnleiter (1)
- Heart tissue culture (1)
- Hemoglobin structure (1)
- Hodgkin–Huxley models (1)
- Homogenization (1)
- Hämoglobinstruktur (1)
- Induced pluripotent stem cells (1)
- Inotropic compounds (1)
- Ion channels (1)
- Körpertemperatur (1)
- LPS (1)
- Mechanical simulation (1)
- Mechanische Beanspruchung (1)
- NONOate (1)
- Natriumhypochlorit (1)
- Nitric Oxide (1)
- Nitric Oxide Donor (1)
- Organkultur (1)
- Pflanzenstress (1)
- Pharmacology (1)
- Recombinant activated protein C (1)
- Reconstruction (1)
- Red blood cell storage (1)
- Sepsis (1)
- Septic cardiomyopathy (1)
- Sonde (1)
- Tissue Engineering (1)
- Ureter (1)
- Wasserstoffperoxid (1)
- Wundheilung (1)
- actin cytoskeleton (1)
- activated nanostructured carbon (1)
- aktivierte nanostrukturierte Kohlenstofffaser (1)
- carbonized rice husk (1)
- cardiomyocyte biomechanics (1)
- contractile tension (1)
- date palm tree (1)
- epithelization (1)
- hiPS cardiomyocytes (1)
- human dermal fibroblasts (1)
- kontraktile Spannung (1)
- plant stress (1)
- plasma generated ions (1)
- rhAPC (1)
- wound healing (1)
Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here.
Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.
The importance of the availability of stored blood or blood cells, respectively, for urgent transfusion cannot be overestimated. Nowadays, blood storage becomes even more important since blood products are used for epidemiological studies, bio-technical research or banked for transfusion purposes. Thus blood samples must not only be processed, stored, and shipped to preserve their efficacy and safety, but also all parameters of storage must be recorded and reported for Quality Assurance. Therefore, blood banks and clinical research facilities are seeking more accurate, automated means for blood storage and blood processing.
Replacement tissues, designed to fill in articular cartilage defects, should exhibit the same properties as the native material. The aim of this study is to foster the understanding of, firstly, the mechanical behavior of the material itself and, secondly, the influence of cultivation parameters on cell seeded implants as well as on cell migration into acellular implants. In this study, acellular cartilage replacement material is theoretically, numerically and experimentally investigated regarding its viscoelastic properties, where a phenomenological model for practical applications is developed. Furthermore, remodeling and cell migration are investigated.
Biocompatibility, flexibility and durability make polydimethylsiloxane (PDMS) membranes top candidates in biomedical applications. CellDrum technology uses large area, <10 µm thin membranes as mechanical stress sensors of thin cell layers. For this to be successful, the properties (thickness, temperature, dust, wrinkles, etc.) must be precisely controlled. The following parameters of membrane fabrication by means of the Floating-on-Water (FoW) method were investigated: (1) PDMS volume, (2) ambient temperature, (3) membrane deflection and (4) membrane mechanical compliance. Significant differences were found between all PDMS volumes and thicknesses tested (p < 0.01). They also differed from the calculated values. At room temperatures between 22 and 26 °C, significant differences in average thickness values were found, as well as a continuous decrease in thicknesses within a 4 °C temperature elevation. No correlation was found between the membrane thickness groups (between 3–4 µm) in terms of deflection and compliance. We successfully present a fabrication method for thin bio-functionalized membranes in conjunction with a four-step quality management system. The results highlight the importance of tight regulation of production parameters through quality control. The use of membranes described here could also become the basis for material testing on thin, viscous layers such as polymers, dyes and adhesives, which goes far beyond biological applications.
Tests with palm tree leaves have just started yet and scan data are in the process to be analyzed. The final goal of future project for palm tree gender and species recognition will be to develop optical scanning technology to be applied to date palm tree leaves for in–situ screening purposes. Depending on the software used and the particular requirements of the users the technology potentially shall be able to identify palm tree diseases, palm tree gender, and species of young date palm trees by scanning leaves.
Changes in intestinal microflora in rats induced by oral exposure to low lead (II) concentrations
(2015)
The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.