Tim Wendlandt, Claudia Koch, Beate Britz, Anke Liedek, Nora Schmidt, Stefan Werner, Yuri Gleba, Farnoosh Vahidpour, Melanie Welden, Arshak Poghossian, Michael Josef Schöning
- Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.
MetadatenVerfasserangaben: | Tim Wendlandt, Claudia Koch, Beate Britz, Anke Liedek, Nora Schmidt, Stefan Werner, Yuri Gleba, Farnoosh Vahidpour, Melanie Welden, Arshak PoghossianORCiD, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/doi.org/10.3390/v15091951 |
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ISSN: | 1999-4915 |
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Titel des übergeordneten Werkes (Englisch): | Viruses |
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Verlag: | MDPI |
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Verlagsort: | Basel |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Erscheinungsjahr: | 2023 |
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Datum der Erstveröffentlichung: | 19.09.2023 |
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Datum der Publikation (Server): | 21.09.2023 |
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Freies Schlagwort / Tag: | biosensor; enzyme cascade; glucose oxidase (GOx); horseradish peroxidase (HRP); tobacco mosaic virus (TMV); turnip vein clearing virus (TVCV) |
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Jahrgang: | 9 |
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Ausgabe / Heft: | 15 |
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Umfang: | Artikel 1951 |
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Bemerkung: | This article belongs to the Special Issue "Tobamoviruses 2023" |
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Link: | https://doi.org/10.3390/v15091951 |
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Zugriffsart: | weltweit |
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Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
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| FH Aachen / INB - Institut für Nano- und Biotechnologien |
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open_access (DINI-Set): | open_access |
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collections: | Verlag / MDPI |
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| Open Access / Gold |
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Lizenz (Deutsch): | Creative Commons - Namensnennung |
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