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Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers
- Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.
Verfasserangaben: | Melanie Welden, Arshak PoghossianORCiD, Farnoosh Vahidpour, Tim Wendlandt, Michael Keusgen, Christina Wege, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.3390/bios12010043 |
ISSN: | 2079-6374 |
Titel des übergeordneten Werkes (Englisch): | Biosensors |
Verlag: | MDPI |
Verlagsort: | Basel |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Erscheinungsjahr: | 2022 |
Datum der Erstveröffentlichung: | 14.01.2022 |
Datum der Publikation (Server): | 17.02.2023 |
Freies Schlagwort / Tag: | bi-enzyme biosensor; capacitive field-effect sensor; enzyme-logic gate; penicillinase; tobacco mosaic virus (TMV); urease |
Jahrgang: | 12 |
Ausgabe / Heft: | 1 |
Umfang: | Artikel 43 |
Bemerkung: | This article belongs to the Special Issue "Biosensors: 10th Anniversary Feature Papers" |
Link: | https://doi.org/10.3390/bios12010043 |
Zugriffsart: | weltweit |
Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Chemie und Biotechnologie |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
open_access (DINI-Set): | open_access |
collections: | Verlag / MDPI |
Open Access / Gold | |
Lizenz (Deutsch): | Creative Commons - Namensnennung |